...Spreaders (VWR, 60828-680) 5 mL and 10 mL Serological pipettes Ice slurry (Ice bucket with ice and water to create...until completely thawed. Chill a box of 200 µL micropipette tips in a -20 °C freezer. Aliquot 3 mL SOC into... two separate transformations). Pro-Tip Do not pipette repeatedly or mix when removing SOC containing ... Use one scraper for all plates. Use two 10 mL pipettes (one for dispensing cold LB and one for removing...). Pro-Tip Scraped bacteria in LB can clog the pipette either mix gently up and down avoiding introducing...

...be worn at all times. Use plastic pipettes in place of glass pipettes or needles. Liquid waste should be...OPTI-MEM In a polypropylene tube, add OPTI-MEM first. Pipette FuGENE® directly into the OPTI-MEM – do not allow...tube from step c for a total volume of 100 μL. Pipette master mix directly into the liquid and not onto...cells. Swirl to disperse mixture evenly. Do not pipette or swirl too vigorously, as you do not want to ...fresh DMEM + 10% FBS + penicillin/streptomycin. Pipette the media onto the side of the plate so as not ...

...Mulitchannel Pipetting Technique Learn how to pipette multiple samples at once using a multichannel pipette and...and avoid inconsistent pipetting with our simple tips Bacterial Transformations We walk you through a standard...

...use Watch the Video! Pipetting Learn about selecting the correct pipettor and pipette tip, how to dispense...dispense liquids, and how to handle the pipette Watch the Video! Centrifugation Learn about selecting and ...

...digest results. Equipment Electrophoresis chamber Pipetman Reagents Liquid DNA aliquot of your plasmid of...instructions) Gel loading dye Electrophoresis buffer Pipet tips Procedure Select restriction enzymes to digest....2-0.5 µL of enzyme because it is difficult to pipette less volume than this; 0.2-0.5 µL will likely be... more enzyme is usually better. Mix gently by pipetting. Incubate tube at appropriate temperature (usually...

...minimizes the number of small volumes you need to pipet. If you are using the same template DNA for all ...wish to perform and add 10% extra to account for pipetting error. In this example, we have 7 different reactions... (0.14µl) = 1.54µl Mix the contents by gently pipetting up and down several times. Keep tube on ice. Add...mix so you know that the primers have been added, pipet the forward primer onto one side of the tube wall...

...chromosomes. Collect the supernatant into a new tube by pipetting or carefully pouring. Optional: Add 5 μL of 2 ...particles Bottom phase - Organic phase (protein) Pipet the aqueous DNA layer and place it in a new microfuge...the tube after the 70% ethanol wash. You can also pipet the supernatant out of the tube if you are worried...

... process works. Equipment Autoclave Water bath Pipetman Reagents 37 g pre-mixed powder consisting of: ...10 - 15 mL of agar. Autoclavable flasks Sterile pipettes Ice bucket to hold antibiotic Antibiotic at 1000...pouring. Measure your desired amount of agar with a pipete for the first plate to get a good idea of what ...

...stab or glycerol stock . Using a sterile loop, pipette tip or toothpick, touch the bacteria growing within...plate, to create streak #2. Using a third sterile pipette tip, toothpick, or sterilized loop, drag through...

...restriction digest. Equipment Electrophoresis chamber Pipetman Pipet tips Reagents Liquid DNA aliquot of your plasmid...

...autoclave. Only mechanical pipetting should be done in the laboratory. Don’t mouth pipette! There may be some ...

... formulation buffer and sample and make sure to pipet back and forth a few times to mix the iodixanol ...buffer. Use a P1000 to the bottom of the filter and pipette up/down and wash off the walls of the filter to...

... the tube and use a sterile loop, toothpick or pipette tip to scrape some of the frozen bacteria off of...

...needed because several microliters can be lost in pipetting. For example, if you want to load 1.0 μg in 10μL...

... LB in a 2 L Erlenmeyer flask. Using a sterile pipette tip or toothpick, select a single colony from your...