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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 48 results for: RING

Showing: 21 - 40 of 48 results

  1. General Transfection

    Type
    Protocol
    ...hydroxide 0.22 μm polyethersulfone (PES) filter Syringes for filtering Reagent Preparation DMEM Complete: 10% ...of powder into 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...

  2. Lentivirus Production

    Type
    Protocol
    ... of powder in 100 mL of deionized water. While stirring, slowly add hydrochloric acid until the solution...DNA tube. Incubate the mixture 12–15 min at RT. During the incubation, add 10 mL of DMEM Complete to a... pellet any packaging cells that were collected during harvesting. Filter supernatant through a 0.45 μm...

  3. Using a Light Microscope Protocol

    Type
    Protocol
    ...knobs on the side of the microscope. You may be wondering why there are multiple objective lenses. Each ...with the lighting, use the coarse focus knob to bring your sample into focus. Once your sample is in focus...easily see your sample. Then use the focus knobs to bring the sample into focus. Pro-Tip As you increase your...

  4. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...reference AAV of known titer, 100837-AAV1, and by measuring the titer of samples obtained from academic viral...transparent film. Centrifuge at 3,000 rpm for 2 min to bring the sample to the bottom of the tube. Run the following...amount of background signal that is most evident during initial PCR cycles. This background signal must...

  5. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ... the sample is determined. Sharing speeds science. We believe that sharing the full details of our protocols...and ImageJ software. The sample is separated by denaturing polyacrylamide gel electrophoresis alongside ...

  6. Western Blot

    Type
    Protocol
    ...SDS-PAGE gel, and immunoblotting. Sharing speeds science. We believe that sharing the full details of our protocols...buffer is suitable for most proteins but more stringent buffers and a sonication step may be required ...

  8. Protocol - How to Streak a Plate

    Type
    Protocol
    ...Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture Introduction... you have single colonies, you can proceed to Recovering Plasmid DNA or use the individual colonies for...

  9. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...with the razor blade. This is especially important during the DNA purification step, as many kits cannot ...used to determine how much of each buffer to add during the DNA isolation step. Finally, you will want ...

  10. Ligation Independent Cloning

    Type
    Protocol
    ... cloning methods. In traditional cloning, base-pairing in the short overlapping regions (usually 4 bp)...annealed but nicked vector product is then repaired during the replication cycle. Empty vectors for LIC typically...

  11. Lab Safety for Biosafety Levels One and Two

    Type
    Protocol
    ...Guidelines BSL-1 Guidelines Before You Work Right after entering the lab, put on the proper personal protective...concentration of 10% bleach for 30 minutes before pouring down the drain. Solid BSL-2 waste can be collected...

  12. Immunocytochemistry

    Type
    Protocol
    ...cells, target proteins, etc. Sharing speeds science. We believe that sharing the full details of our protocols...

  14. Lentivirus ddPCR Titration

    Type
    Protocol
    ...immediately or stored at -20 °C until ready to use. Preparing for ddPCR Thaw samples, primers/probe mixes, and...samples on ice or pre-chilled 96-well freezer blocks during use. To reduce the risk of contaminating reagents...

  15. Protocol - How to Design Primers

    Type
    Protocol
    ...inosine) instead of a regular nucleotide for broader pairing capabilities. Taking into consideration the information...

  19. Weighing Reagents Protocol

    Type
    Protocol
    ...your container. Make sure that any spills produced during the protocol are cleaned up. Discard the weighing...

  20. Water Bath Protocol

    Type
    Protocol
    ...bath is a piece of laboratory equipment that helps bring your materials to a particular temperature, catalyze...
Showing: 21 - 40 of 48 results