Addgene

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AAV Purification by Iodixanol Gradient Ultracentrifugation

Type

Protocol

...Right image adapted by permission from Macmillan Publishers Ltd: Nature Protocols Grieger, Joshua C., Vivian...
Protocol - How to Run an Agarose Gel

Type

Protocol

... which will disrupt the gel. Any bubbles can be pushed away from the well comb or towards the sides/edges...
Handling Plasmids from Addgene - Purifying Plasmid DNA

Type

Protocol

...vortex at this stage or the genomic DNA will become sheared and will therefore contaminate your purified plasmid...
Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

Type

Protocol

...to insert in the ligation reaction. Isolate the Finished Plasmid Finally, you will need to pick individual...
Virus Protocol - Generating Stable Cell Lines

Type

Protocol

...deliver antibiotic resistance. This protocol was established using 293T cells but can be adapted to alternative...
Plasmid Cloning by PCR (with Protocols)

Type

Protocol

...amount of recipient plasmid to insert. Isolate the Finished Plasmid Finally, you will need to pick individual...
AAV ddPCR Titration

Type

Protocol

...dUTP, Bio-Rad,1863023 10% Pluronic F-68, Thermo Fisher, 24040032 Droplet generation oil, Bio-Rad, 1863005...
CRISPR Library Amplification

Type

Protocol

...is expected to work. 50 mL Falcon Conical tubes (Fisher, 14-432-22) Reagent Preparation Prepare, sterilize...

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