Skip to main content
Addgene
Showing: 21 - 35 of 35 results

  1. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...lentiviral vectors deliver mammalian antibiotic resistance (e.g., puromycin, blasticidin), which enables...not all lentiviral vectors deliver antibiotic resistance. This protocol was established using 293T cells...transgene expression. As polyclonal populations of resistant cells start proliferating and the individual wells...

  2. Plasmid Cloning by PCR (with Protocols)

    Type
    Protocol
    ...molecular cloning consist of: Leader Sequence: Extra base pairs on the 5' end of the primer assist with restriction...selecting restriction sites, you should use a DNA analysis tool, such as Addgene’s Sequence Analyzer , to...

  3. Kit Free RNA Extraction

    Type
    Protocol
    ...general steps of this protocol include homogenization/lysis of cells or tissues, extraction of RNA, precipitation...isolation will depend on how effective your cell lysis protocol is. While simple homogenization is effective...

  4. CRISPR Library Amplification

    Type
    Protocol
    ... on incremental, ordered cell lysis. E. coli cells are subject to lysis by freeze-thaw if not suspended...sufficient DNA for use, pool samples, and continue with analysis. “Sufficient” DNA will vary upon intended use....

  5. Water Bath Protocol

    Type
    Protocol
    ...allow the temperature to stabilize. Use water resistant markers, such as permanent markers, to label your...water bath, be sure to use a marker that is also resistant to chemicals such as ethanol. Writing on the tops...

  6. DNA Quantification

    Type
    Protocol
    ...such as TE). You will get much more accurate and consistent readings from DNA in a buffered solution. Reference...

  7. Protocol - How to Design Primers

    Type
    Protocol
    ...Reaction Plasmid Cloning by PCR Agarose Gel Electrophoresis Primer Design for PCR Oligonucleotide primers...

  10. Ligation Independent Cloning

    Type
    Protocol
    ...linearized vector from the reaction mixture by gel electrophoresis followed by gel purification . The cut vector...

  12. Pipetting Protocol

    Type
    Protocol
    ...multiple “stops” on the plunger, where you feel resistance while pressing down on the pipette. Stop at the...

  15. Lentivirus ddPCR Titration

    Type
    Protocol
    ... Benzonase and harvest cells Day 4+: ddPCR and analysis Equipment Class II, Type A2 Biological Safety ...
Showing: 21 - 35 of 35 results