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We narrowed to 34 results for: Tub;

Showing: 21 - 34 of 34 results

  1. Weighing Reagents Protocol

    Type
    Protocol
    ...microcentrifuge tube on the balance and then tare it. Then add the material directly to the tube to weigh it...paper Balance Scoopula or spatula Container (beaker, tube, flask, etc.) Reagents Material that will be weighed...They also help you transfer the material to your tube or container. Pro-Tip If you’re using weighing paper...

  2. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ... into individual tubes and then add the appropriate amount of Master Mix to each tube. This will save ... double digest finder tool from NEB . In a 1.5mL tube combine the following: DNA Restriction Enzyme(s)...usually better. Mix gently by pipetting. Incubate tube at appropriate temperature (usually 37 °C) for 1...

  3. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...good idea to take a fresh tube, thaw it one time and aliquot individual tubes of 5, 10 or 20μL for storage...use. Combine the following in a PCR or Eppendorf tube: Vector DNA Insert DNA Ligase Buffer (1μL/10μL reaction...set up ligations in the future you can thaw a new tube that you know has only been thawed once before. ...

  4. AAV Production in HEK293 Cells

    Type
    Protocol
    ...membrane. Aliquot 500–1000 μL into sterile tubes. Store the tubes at -80 °C. After thawing, the solution ... for up to 2 months. After 2 months, discard the tube and thaw a new working stock. 40% POLYETHYLENE GLYCOL... min at 4 °C. Transfer the cleared lysate to the tube containing the resuspended virus from step 12 above...

  5. Immunocytochemistry

    Type
    Protocol
    ...medium Laboratory wipes 15 mL conical tubes 50 mL conical tubes Before Starting Refer to the manufacturer's...channel pipette Reagents 1X PBS Microcentrifuge tubes Sterile Poly-D-lysine coated coverslips HeLa cells...

  6. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...Microcentrifuge tubes, Neptune 3745.X 6-well tissue culture treated dish, Corning 3516 15 mL conical tubes, VWR ...polybrene. Mix well by pipetting or inverting the tube. Aliquot 1 mL of cell suspension (i.e., 50,000 cells...

  7. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ... Gel loading tips, Corning 4853 Microcentrifuge tubes, Neptune 3745.X PBS, 1X pH 7.4, VWR 45000-446 250...PBS Transfer 5 µL of each standard to a microfuge tube. Add 8 µL of PBS to a total volume of 13 µL . Prepare...Transfer 5 µL of recombinant antibody to a microfuge tube. Add 8 µL of PBS to total 13 µL . Prepare 1X MOPS...

  8. Western Blot

    Type
    Protocol
    ... buffer e.g., RIPA lysis buffer Microcentrifuge tubes BCA Assay, Thermo Fisher 23227 β-mercaptoethanol...1 mL of 1X PBS and transfer to a microcentrifuge tube. Centrifuge for 5 min at 100 x g . Carefully remove... Transfer supernatant to a clean microcentrifuge tube. Use the lysate immediately or store at -80 °C until...

  9. Protocol - How to Inoculate a Bacterial Culture

    Type
    Protocol
    ...When ready to grow your culture, add liquid LB to a tube or flask and add the appropriate antibiotic to the... you will usually want to start 2 mL in a falcon tube, but for larger preps you might want to use as much...

  10. Fluorescence Titering Assay

    Type
    Protocol
    ...Microcentrifuge tubes, Neptune 3745.X 6-well tissue culture treated dish, Corning 3516 15 mL conical tubes, VWR ...

  11. Colony Formation Titering Assay

    Type
    Protocol
    ...Microcentrifuge tubes, Neptune 3745.X 6-well tissue culture treated dish, Corning 3516 15 mL conical tubes, VWR ...

  12. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...DNase buffer Nuclease-free water Microcentrifuge tubes 96-well optical plate Pipette tips General Considerations...for 2 min to bring the sample to the bottom of the tube. Run the following protocol in your qPCR instrument...

  13. Pouring LB Agar Plates

    Type
    Protocol
    ...potential contaminants, certain types of spores will stubbornly survive even under the harsh conditions in the... leave room for your bottle of molten gel mix, a tube rack containing the appropriate antibiotics, and...

  14. Gibson Assembly Protocol

    Type
    Protocol
    ... scar between joined fragments. Fewer steps. One tube reaction. Can combine many DNA fragments at once...
Showing: 21 - 34 of 34 results