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Addgene
Showing: 21 - 29 of 29 results
  1. Ligation Independent Cloning

    Type
    Protocol
    ... your chances of success. Set up a vector only control with water instead of the insert. Incubate the ...
  2. Using a Light Microscope Protocol

    Type
    Protocol
    ...resolution when your image is in focus, which you can control using the focus knobs on the side of the microscope...
  3. General Transfection

    Type
    Protocol
    ...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container Reagents DMEM high ...
  4. Lentivirus Production

    Type
    Protocol
    ...channel pipette Ice bucket CO 2 incubator Pipet controller Hazardous waste container pH meter Stir plate...
  5. AAV ddPCR Titration

    Type
    Protocol
    ... and positive droplets (blue). The no template control (NTC) should be close to zero (B08). At Addgene...
  6. CRISPR Library Amplification

    Type
    Protocol
    ...according to the actual number. Required Quality Control (QC) Pooled libraries can be challenging and expensive...
  7. AAV Production in HEK293 Cells

    Type
    Protocol
    ... cell counter Ice bucket CO 2 incubator Pipet controller Hazardous waste container pH meter Stir plate...
Showing: 21 - 29 of 29 results