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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 44 results for: des

Showing: 21 - 40 of 44 results

  1. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...be pushed away from the well comb or towards the sides/edges of the gel with a pipette tip. Place newly...Note: Loading buffer serves two purposes: 1) it provides a visible dye that helps with gel loading and ...Always Run to Red. Turn OFF power, disconnect the electrodes from the power source, and then carefully remove...-30 mins, replace EtBr solution with water and destain for 5 mins. Using any device that has UV light,...

  2. AAV Production in HEK293 Cells

    Type
    Protocol
    ...may also like... AAV Viral Service Viral Vector Guides Virus Blog Posts Molecular Biology Reference Introduction...recheck the pH to prevent over or undershooting the desired pH. Allow the solution to mix for 10 min and then...2-3 minutes for cells to detach. Gently tap the sides of the CS2 to help detach the cells, add 200 mL ...for ~72 h. Harvest cells and media by tapping the sides of the CS5. Cells should detach easily. Transfer...

  3. CRISPR Library Amplification

    Type
    Protocol
    ...library during amplification. This protocol is designed to be as general as possible but note that individual...activating pulse and follow all specifications described in the equipment manual. Immediately add 1 mL ...µl aliquots of cell/DNA mixture used (8 in the described protocol) using a new vented falcon tube for every...than Nanodrop, although most protocols have been designed with Nanodrop as the quantification method. If...

  4. General Transfection

    Type
    Protocol
    ...Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service Introduction This protocol describes a general...recheck the pH to prevent over or undershooting the desired pH. Allow the solution to mix for 10 min and then...

  5. Protocol - How to Ligate Plasmid DNA

    Type
    Protocol
    ...phosphodiester linkages, which permanently join the nucleotides together. After ligation, the insert DNA is physically... likely to ligate to itself rather than to the desired insert. If you are in this situation, it is important...reactions. Because ligase buffer contains ATP, which degrades upon freeze/thaw cycles, it is a good idea to ...

  6. Pipetting Protocol

    Type
    Protocol
    ...maximum possible setting on the pipette, set the desired volume of the liquid on the pipette. Depending ...of the pipette. Select the pipette tip that is designed to fit your pipette. To load a tip onto a pipette...from the container making sure not to touch the sides of the container with the pipette. Discard the pipette...

  7. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...Last Update: Feb. 8, 2018 Equipment Desktop microcentrifuge Desktop vortexer Vacuum (optional) Reagents... several μg/μL. The protocol below is meant to describe the general procedure for purifying plasmid DNA...

  8. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ...with Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service...dilutions and pick the population that has the most desirable level of expression. Over time, transgene expression...from Feng Zhang (Addgene plasmid #52962 ) and is described in Improved vectors and genome-wide libraries ...

  9. AAV ddPCR Titration

    Type
    Protocol
    ...Vector Guides Virus Blog Posts Molecular Biology Reference Introduction This protocol describes how to...QuantaSoft software to set up a new plate layout. Designate the sample name, experiment type, supermix type...

  10. DNA Quantification

    Type
    Protocol
    ...samples, place 1-2µL of mini-prepped DNA onto the pedestal. Close the lid and click measure, be sure to record...Repeat for each sample. Notes: Keep in mind that despite the accuracy of the NanoDrop, if two consecutive...

  11. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ...and with a clean, sterile razor blade, slice the desired DNA fragment from the gel. Notes: To protect the...down on the UV box and trim the top, bottom and sides with the razor blade. This is especially important...

  12. Lentivirus Production

    Type
    Protocol
    ...Lentivirus Production You may also like... Viral Vector Guides Mol Bio Protocols Introduction This protocol can...recheck the pH to prevent over or undershooting the desired pH. Allow the solution to mix for 10 min and then...

  13. AAV Purification by Iodixanol Gradient Ultracentrifugation

    Type
    Protocol
    ...Ultracentrifugation You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Introduction...preparation. The 15% iodixanol step has 1M NaCl to destabilize ionic interactions between macromolecules. The...

  14. Pouring LB Agar Plates

    Type
    Protocol
    ...-agar 1 L Sterile H 2 O Sterile plates of your desired size - we usually use 60 mm x 15 mm plates which...next to the flame and begin pouring. Measure your desired amount of agar with a pipete for the first plate...

  15. Kit Free RNA Extraction

    Type
    Protocol
    ... Biology Reference Introduction This protocol describes how to extract RNA from tissues or cells without...precipitation, and resuspension. This protocol provides two options: using Solution D (see reagent section...

  16. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Guide Virus Blog Posts Introduction This protocol describes how to use droplet digital PCR (ddPCR) to titer...QuantaSoft software to set up a new plate layout. Designate the sample name, experiment type, supermix type...

  19. Fluorescence Titering Assay

    Type
    Protocol
    ...for Lentivirus You may also like... Viral Vector Guides Virus Blog Posts Mol Bio Protocols Viral Service...
Showing: 21 - 40 of 44 results