...the DG8 gasket, making sure that it is secure. Transfer the cartridge holder to the droplet generator....while slowly and gently aspirating the droplets. Transfer the droplets to a prechilled PCR plate. Pro-Tip... 98 10 2 1 Hold 4 ∞ 2 1 After PCR is complete, transfer the plate to the Droplet Reader. Open the QuantaSoft...

...cells. Extract RNA from the homogenized sample(s).Transfer tissue/cell lysate to a 4 mL tube. Add the following...the cold room may damage it. Using a pipettor, transfer the top, aqueous phase to a new RNAse-free tube...

... of Plasmids You may also like... Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture... not need to obtain more competent cells and retransform. Bacteria on an LB agar plate can be stored at...

...Digest of Plasmid DNA DNA Ligation Bacterial Transformation Summary Oligo overlap cloning can be used anytime...the vector with 0.75-6 ng of annealed oligos). Transform 2-3μL into your favorite competent bacteria and...

...cross-contamination with other substances. They also help you transfer the material to your tube or container. *Pro-Tip...weighed out the correct amount of your reagent, transfer it into your container. Make sure that any spills...

...Bacterial Culture You may also like... Bacterial Transformation Agarose Gel Electrophoresis Agarose Gel DNA...optional) Reagents Overnight culture of bacteria transformed with your plasmid Resuspension buffer Denaturing...

...x (0.220 L) = 8.14 g pre-mixed LB-agar powder. Transfer the LB-agar powder you’ve measured out into an...molten agar from boiling over in the autoclave. Transfer the sterile water (in our case 220 mL) to the ...

...also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture...

...brands and lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to...

...You can purchase master mix or make your own. Transform bacteria with the DNA and screen for the correct...

...you know expresses the protein, such as cells transfected with a plasmid to express the protein of interest...

...brands and lots of FBS can promote or inhibit transfection. Test a variety of brands and lots of FBS to...

...sample (do not vortex) Incubate 30 minutes at 37°C Transfer to ice ** Critical: do NOT treat your plasmid ...