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Addgene

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We narrowed to 33 results for: prep

Showing: 21 - 33 of 33 results

  1. Antibody Validation Using the Indirect ELISA Method

    Type
    Protocol
    ...reagents to room temperature. Procedure Section 1: Prepare the Antigen Standard and coat the plate Dilute ... antigen to determine the ideal concentration. Prepare serial dilutions of the purified antigen as follows...overnight at 4 °C . Section 2: Block the plate Prepare the wash buffer (0.05% Tween-20 in PBS) as follows...wash, aspirate the wash buffer from the wells. Prepare the blocking buffer (1% BSA in PBS) as follows:...wash, aspirate the wash buffer from the wells. Prepare antibody dilution buffer (0.1% BSA in PBS) as follows...

  2. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...conditioned medium prepared above to make a new cell solution at a concentration of 5 cells/mL. Prepare approximately...culture dish, Corning 430167 (optional) Reagent Preparation DMEM Complete: 10% v/v FBS and 4 mM L-alanyl-...a hemocytometer or other cell counter. Pro-Tip Prepare a relatively dilute cell solution (<10 6 cells/.... If you choose not to use conditioned medium, prepare this cell solution in fresh complete medium. This...

  3. Video Library

    Type
    Protocol
    ...Purification Purify adeno-associated virus from a preparation produced in mammalian cells AAV Purification ... Inoculating a Liquid Bacterial Culture How to prepare and grow bacteria in a liquid medium Inoculating...physical titer of your adeno-associated virus preparation. AAV Titration by qPCR Using SYBR Green Technology...

  4. General Transfection

    Type
    Protocol
    ... (PES) filter Syringes for filtering Reagent Preparation DMEM Complete: 10% v/v FBS and 4 mM L-alanyl-...empirically determined for each new batch of 1 mg/mL PEI prepared. There may be variation between batches of PEI...empirically determined. Once a batch of PEI is prepared, transfect cells with a fluorescent plasmid using...

  5. Immunocytochemistry

    Type
    Protocol
    ...primary antibodies raised in a mouse. Reagent Preparation Permeabilization buffer: Dilute 20 µL of Triton...100 in 50 mL PBS. 300 nM DAPI working solution: Prepare a 300 µM DAPI stock solution by diluting 2.1 µL...DAPI solution to 100 µL PBS. Protect from light. Prepare a 300 nM DAPI working solution by diluting 5 µL...

  6. Lentivirus Production

    Type
    Protocol
    ...plasmid encoding your insert of interest Reagent Preparation DMEM Complete (also known as D10): 10% v/v FBS...Incubate the cells at 37 °C, 5% CO 2 for ~20 h. Prepare a mixture of the three transfection plasmids into...empirically determined. Once a batch of PEI is prepared, transfect cells with a fluorescent plasmid using...

  7. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...their resistance genes, as one does not need to prepare separate batches of antibiotic-containing agar....or other antibiotic resistance) Procedure Day 1 Prepare carbenicillin to a concentration of 1 mg/mL – 4...

  8. Centrifugation

    Type
    Protocol
    ...many different experimental procedures, such as minipreps and RNA extractions. Plus, centrifuges are useful...

  10. Using a Light Microscope Protocol

    Type
    Protocol
    ...image of that object! Equipment Light Microscope Prepared Slides (or other sample that can fit on a microscope...

  13. Kit Free RNA Extraction

    Type
    Protocol
    ...Protocol Before starting this protocol, make sure to prepare solution D (see reagent section above for the recipe...
Showing: 21 - 33 of 33 results