We narrowed to 28 results for: she

1. AAV Purification by Iodixanol Gradient Ultracentrifugation

   Type

   Protocol

   ...Right image adapted by permission from Macmillan Publishers Ltd: Nature Protocols Grieger, Joshua C., Vivian...

2. Protocol - How to Run an Agarose Gel

   Type

   Protocol

   ... which will disrupt the gel. Any bubbles can be pushed away from the well comb or towards the sides/edges...

3. Handling Plasmids from Addgene - Purifying Plasmid DNA

   Type

   Protocol

   ...vortex at this stage or the genomic DNA will become sheared and will therefore contaminate your purified plasmid...

4. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

   Type

   Protocol

   ...to insert in the ligation reaction. Isolate the Finished Plasmid Finally, you will need to pick individual...

5. Virus Protocol - Generating Stable Cell Lines

   Type

   Protocol

   ...deliver antibiotic resistance. This protocol was established using 293T cells but can be adapted to alternative...

6. Plasmid Cloning by PCR (with Protocols)

   Type

   Protocol

   ...amount of recipient plasmid to insert. Isolate the Finished Plasmid Finally, you will need to pick individual...

7. AAV ddPCR Titration

   Type

   Protocol

   ...dUTP, Bio-Rad,1863023 10% Pluronic F-68, Thermo Fisher, 24040032 Droplet generation oil, Bio-Rad, 1863005...

8. CRISPR Library Amplification

   Type

   Protocol

   ...is expected to work. 50 mL Falcon Conical tubes (Fisher, 14-432-22) Reagent Preparation Prepare, sterilize...