We narrowed to 24 results for: ttl

1. Handling Plasmids from Addgene - Purifying Plasmid DNA

   Type

   Protocol

   ...centrifuge tube, aliquot it into several tubes/bottles. Remove the supernatant and resuspend the bacteria...

2. Virus Protocol - Generating Stable Cell Lines

   Type

   Protocol

   ...stable alternative, such as glutaGRO) To a 500 mL bottle of DMEM high glucose, add 55 mL of heat-inactivated...

3. Plasmid Cloning by PCR (with Protocols)

   Type

   Protocol

   ...from a plasmid or simple template, there is very little chance for mis-priming, so you can use a pretty...

4. CRISPR Library Amplification

   Type

   Protocol

   ... of altering the composition of the library. Bottlenecks, fitness differences, and plasmid recombination...