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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 35 results for: PIP

Showing: 21 - 30 of 35 results

  1. CRISPR Library Amplification

    Type
    Protocol
    ...Spreaders (VWR, 60828-680) 5 mL and 10 mL Serological pipettes Ice slurry (Ice bucket with ice and water to create...until completely thawed. Chill a box of 200 µL micropipette tips in a -20 °C freezer. Aliquot 3 mL SOC into... two separate transformations). Pro-Tip Do not pipette repeatedly or mix when removing SOC containing ... Use one scraper for all plates. Use two 10 mL pipettes (one for dispensing cold LB and one for removing...). Pro-Tip Scraped bacteria in LB can clog the pipette either mix gently up and down avoiding introducing...

  2. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...be worn at all times. Use plastic pipettes in place of glass pipettes or needles. Liquid waste should be...OPTI-MEM In a polypropylene tube, add OPTI-MEM first. Pipette FuGENE® directly into the OPTI-MEM – do not allow...tube from step c for a total volume of 100 μL. Pipette master mix directly into the liquid and not onto...cells. Swirl to disperse mixture evenly. Do not pipette or swirl too vigorously, as you do not want to ...fresh DMEM + 10% FBS + penicillin/streptomycin. Pipette the media onto the side of the plate so as not ...

  3. Video Library

    Type
    Protocol
    ...Mulitchannel Pipetting Technique Learn how to pipette multiple samples at once using a multichannel pipette and...and avoid inconsistent pipetting with our simple tips Bacterial Transformations We walk you through a standard...

  5. Molecular Biology Protocol - Restriction Digest of Plasmid DNA

    Type
    Protocol
    ...digest results. Equipment Electrophoresis chamber Pipetman Reagents Liquid DNA aliquot of your plasmid of...instructions) Gel loading dye Electrophoresis buffer Pipet tips Procedure Select restriction enzymes to digest....2-0.5 µL of enzyme because it is difficult to pipette less volume than this; 0.2-0.5 µL will likely be... more enzyme is usually better. Mix gently by pipetting. Incubate tube at appropriate temperature (usually...

  6. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ...minimizes the number of small volumes you need to pipet. If you are using the same template DNA for all ...wish to perform and add 10% extra to account for pipetting error. In this example, we have 7 different reactions... (0.14µl) = 1.54µl Mix the contents by gently pipetting up and down several times. Keep tube on ice. Add...mix so you know that the primers have been added, pipet the forward primer onto one side of the tube wall...

  7. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ...chromosomes. Collect the supernatant into a new tube by pipetting or carefully pouring. Optional: Add 5 μL of 2 ...particles Bottom phase - Organic phase (protein) Pipet the aqueous DNA layer and place it in a new microfuge...the tube after the 70% ethanol wash. You can also pipet the supernatant out of the tube if you are worried...

  8. Pouring LB Agar Plates

    Type
    Protocol
    ... process works. Equipment Autoclave Water bath Pipetman Reagents 37 g pre-mixed powder consisting of: ...10 - 15 mL of agar. Autoclavable flasks Sterile pipettes Ice bucket to hold antibiotic Antibiotic at 1000...pouring. Measure your desired amount of agar with a pipete for the first plate to get a good idea of what ...

  9. Protocol - How to Streak a Plate

    Type
    Protocol
    ...stab or glycerol stock . Using a sterile loop, pipette tip or toothpick, touch the bacteria growing within...plate, to create streak #2. Using a third sterile pipette tip, toothpick, or sterilized loop, drag through...
Showing: 21 - 30 of 35 results