Addgene

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DNA Quantification

Type

Protocol

...Calculate your DNA sample concentration and purity using Addgene’s DNA Quantification Protocol....
Protocol - How to Design Primers

Type

Protocol

... template region of DNA. They are synthesized chemically by joining nucleotides together. One must selectively...
Personal Protective Equipment (PPE) for BSL-1 and BSL-2 Labs

Type

Protocol

...so at your own risk; you should ensure that any local guidance is also adhered to. None of the authors...
Plasmid Modification by Annealed Oligo Cloning (with Protocols)

Type

Protocol

...added when ordering them or can be performed enzymatically later. Experimental Procedure Digest and purify...
Protocol - How to Perform a Diagnostic Digest

Type

Protocol

...restriction enzymes cleave DNA at specific sequences called restrictions sites. Often, the size of the plasmid...
AAV Purification by Iodixanol Gradient Ultracentrifugation

Type

Protocol

... The arrow indicates the 60–40% interface. The vertical black line indicates the location of the purified...

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