... Agarose Gel Electrophoresis Agarose Gel DNA Purification Streaking and Isolating Bacteria Inoculating...instructions. If you want to perform plasmid purification without using a kit, you can find a protocol...Na-acetate (pH 4.8) Protocol: Generalized DNA Purification Grow an overnight culture of bacteria . Pro-...using. Store DNA at 4°C. Tips and FAQ Plasmid purification kits provide the fastest way to obtain a high...

... template region of DNA. They are synthesized chemically by joining nucleotides together. One must selectively...primers produce inaccurate, nonspecific DNA amplification product, and long primers result in a slower...which creates primer dimers and disrupts the amplification process. When designing, if unsure about what...

...Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated, the antibiotic powder can be dissolved in dH ...test results. Sample Data In all cases below (-) indicates that the tested strain is not supposed to be resistant... resistant to the antibiotic, (+) indicates that the tested strain is supposed to be resistant to the ...

...antibiotic-containing agar. This protocol will focus specifically on selection with carbenicillin; however it ...required for effective over-agar selection has been empirically determined. See selection curve below. Carbenicillin...

...in the photo above has a capacity of 200 g, as indicated by the number above the "Zero" button. Before ...you need less than a gram of material, use an analytical scale. This will provide more accuracy as these...

...materials to a particular temperature, catalyze chemical reactions such as restriction digests needed in...sure to use a marker that is also resistant to chemicals such as ethanol. Writing on the tops of the tubes...

... Recombinant Antibodies Recombinant Antibody Purification Protocol Introduction This protocol describes...mobility of the bands (sample AR0016 in Figure 1) indicates that the samples may not have been processed correctly...

...1) and biosafety level 2 (BSL-2). The BSL-1 classification is for labs working with low-risk microbes ...