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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 48 results for: ica;

Showing: 41 - 48 of 48 results

  1. Handling Plasmids from Addgene - Purifying Plasmid DNA

    Type
    Protocol
    ... Agarose Gel Electrophoresis Agarose Gel DNA Purification Streaking and Isolating Bacteria Inoculating...instructions. If you want to perform plasmid purification without using a kit, you can find a protocol...Na-acetate (pH 4.8) Protocol: Generalized DNA Purification Grow an overnight culture of bacteria . Pro-...using. Store DNA at 4°C. Tips and FAQ Plasmid purification kits provide the fastest way to obtain a high...

  2. Protocol - How to Design Primers

    Type
    Protocol
    ... template region of DNA. They are synthesized chemically by joining nucleotides together. One must selectively...primers produce inaccurate, nonspecific DNA amplification product, and long primers result in a slower...which creates primer dimers and disrupts the amplification process. When designing, if unsure about what...

  3. Pouring LB Agar Plates

    Type
    Protocol
    ...Tetracycline 10 mg/mL 10 µg/mL Notes: Unless otherwise indicated, the antibiotic powder can be dissolved in dH ...test results. Sample Data In all cases below (-) indicates that the tested strain is not supposed to be resistant... resistant to the antibiotic, (+) indicates that the tested strain is supposed to be resistant to the ...

  4. Protocol - Over-Agar Antibiotic Plating

    Type
    Protocol
    ...antibiotic-containing agar. This protocol will focus specifically on selection with carbenicillin; however it ...required for effective over-agar selection has been empirically determined. See selection curve below. Carbenicillin...

  5. Weighing Reagents Protocol

    Type
    Protocol
    ...in the photo above has a capacity of 200 g, as indicated by the number above the "Zero" button. Before ...you need less than a gram of material, use an analytical scale. This will provide more accuracy as these...

  6. Water Bath Protocol

    Type
    Protocol
    ...materials to a particular temperature, catalyze chemical reactions such as restriction digests needed in...sure to use a marker that is also resistant to chemicals such as ethanol. Writing on the tops of the tubes...

  7. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ... Recombinant Antibodies Recombinant Antibody Purification Protocol Introduction This protocol describes...mobility of the bands (sample AR0016 in Figure 1) indicates that the samples may not have been processed correctly...
Showing: 41 - 48 of 48 results