We narrowed to 35 results for: Sis

1. Protocol - How to Ligate Plasmid DNA

   Type

   Protocol

   ...of competent cells and verifies the antibiotic resistance of the plasmid Cut vector - Background due to...

2. Pipetting Protocol

   Type

   Protocol

   ...multiple “stops” on the plunger, where you feel resistance while pressing down on the pipette. Stop at the...

3. AAV Purification by Iodixanol Gradient Ultracentrifugation

   Type

   Protocol

   ...cleanest fractions should be kept and pooled for dialysis. Pool clean fractions. Concentration and buffer...

4. Plasmid Cloning by Restriction Enzyme Digest (with Protocols)

   Type

   Protocol

   ...expression vector. Design (Choosing enzymes) Many DNA analysis tools, including Addgene’s Sequence Analyzer ,...

5. Lentivirus ddPCR Titration

   Type

   Protocol

   ... Benzonase and harvest cells Day 4+: ddPCR and analysis Equipment Class II, Type A2 Biological Safety ...