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Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 31 - 40 of 46 results

  1. Affinity Purification of Recombinant Antibodies with Protein A or Protein G

    Type
    Protocol
    ...specific products or equipment. Inclusion of this information is solely for transparency intended to support...spectrophotometer 37 °C, 5% CO 2 incubator with shaking platform set to 120 rpm 37 °C bead bath Clamp stand and...divide the sample among multiple columns. For information about the total binding capacity for your columns...

  2. Protocol - How to Purify DNA from an Agarose Gel

    Type
    Protocol
    ... Gel Electrophoresis DNA Ligation Background Information Gel purification allows you to isolate and purify...step with the now gel purified DNA. Find more information about DNA quantification here . Tips and FAQ ...

  4. Pipetting Protocol

    Type
    Protocol
    ...containers Reagents Liquid for pipetting Background Information Anatomy of a Pipette This front view photo of...the Volume on the Pipette Although each pipette performs the same function, the numbers are read differently...

  5. Protocol - How to Run an Agarose Gel

    Type
    Protocol
    ...Standard protocol for performing agarose gel electrophoresis, including tips to improve resolution and...Watch the protocol video below to learn how to perform gel electrophoresis. Equipment Casting tray Well...

  6. Lentivirus ddPCR Titration

    Type
    Protocol
    ...Follow this protocol to perform digital droplet PCR (ddPCR) for lentivirus vector titration....Vectors You may also like... Lentivirus Colony Formation Titration Assay Lentivirus Fluorescence Titration...

  7. DNA Quantification

    Type
    Protocol
    ...Restriction Digest of Plasmid DNA Background Information During several different stages of molecular ...

  8. Protocol - How to Design Primers

    Type
    Protocol
    ... capabilities. Taking into consideration the information above, primers should generally have the following...

  9. Protocol - How to Streak a Plate

    Type
    Protocol
    ...also like... Making LB Agar Plates Bacterial Transformation Recovering Plasmid DNA from Bacterial Culture...
Showing: 31 - 40 of 46 results