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Showing: 21 - 40 of 45 results

  1. What is Polymerase Chain Reaction (PCR)

    Type
    Protocol
    ... cooling (denaturing and annealing) cycle. The process became automated with the discovery of a heat resistant...Polymerase (5 units/μL) PCR Machine Agarose Gel Procedure Primer Design and PCR Design Primers. See our ...

  2. Virus Protocol - Generating Stable Cell Lines

    Type
    Protocol
    ... be subjected to multiple freeze-thaw cycles. Procedure Before beginning, determine the optimal dose of...antibiotic. This is the beginning of the selection process, which will begin the selection of a stable cell...

  3. AAV Titration by qPCR Using SYBR Green Technology

    Type
    Protocol
    ...0.15 μL 15 μL Nuclease Free Water 4.7 μL 470 μL Procedure Prepare a plasmid stock of 2x10 9 molecules/μl...contaminating plasmid DNA carried over from the production process (DNase does not penetrate the virion). 5μL sample...

  4. Coomassie Purity Stain of Recombinant Antibodies

    Type
    Protocol
    ... mL of deionized water. Gently invert to mix. Procedure Section 1: SDS-PAGE Add 5 µL of 4X sample buffer... indicates that the samples may not have been processed correctly in the cell (e.g., failure to cleave...

  5. Isolating a Monoclonal Cell Population by Limiting Dilution

    Type
    Protocol
    ...for different cell types. Before starting this procedure, be sure that the cell pool has been sufficiently...well plate gave rise to 15–20 monoclonal lines. Procedure Generating Conditioned Medium (optional): Seed...

  6. AAV ddPCR Titration

    Type
    Protocol
    ...Pluronic F-68 4475 µL Molecular Biology Grade Water Procedure Preparation Before handling any viruses, get materials...Seal’ button. After the plate has been sealed, proceed to thermocycling. Thermal Cycling Run the following...

  7. Lentivirus ddPCR Titration

    Type
    Protocol
    ...15 mL DMEM Complete 3 µL of 250 U/µL benzonase Procedure Transducing Cells Thaw the virus on ice. Prepare...Seal’ button. After the plate has been sealed, proceed to thermocycling. Thermal Cycling Run the following...

  8. Protocol - pLKO.1 – TRC Cloning Vector

    Type
    Protocol
    ...found at the Broad Institute's TRC shRNA Design Process . To minimize degradation of off-target mRNAs, ...institution-specific instructions. You must follow safety procedures and work in an environment (e.g. BL2+) suitable...

  11. Weighing Reagents Protocol

    Type
    Protocol
    ....) Reagents Material that will be weighed out Procedure The balance in the photo above has a capacity ...

  12. Water Bath Protocol

    Type
    Protocol
    ... bath weights and floats Reagents None needed Procedure Ensure that the water bath and water bath cover...

  16. Fluorescence Titering Assay

    Type
    Protocol
    ... supernatants to multiple freeze-thaw cycles. Procedure Seed 75,000 cells into each well of a 6-well dish...

  18. General Transfection

    Type
    Protocol
    ...the best ratio of mass DNA:mass PEI determined. Procedure Seed HEK293T cells at 3.8x10 6 cells per plate...

  19. Gibson Assembly Protocol

    Type
    Protocol
    ...reaction. Can combine many DNA fragments at once. Procedure Design your plasmid and order primers (see figure...

  20. Immunocytochemistry

    Type
    Protocol
    ...stock solution into 5 mL PBS. Protect from light. Procedure Section 1: Seeding cells Place a sterile poly-...
Showing: 21 - 40 of 45 results