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Addgene
Showing: 81 - 100 of 3757 results

  1. pAAV-CAG-FLEX-EGFP

    Plasmid
    #59331
    Purpose
    Expresses EGFP
    Depositor
    Ian Wickersham
    Has Service
    AAV1
    Insert
    EGFP
    Use
    AAV and Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only

  2. pAAV-CAG-FLEX-splitTVA-EGFP

    Plasmid
    #59332
    Purpose
    Expresses splitTVA-P2A-EGFP
    Depositor
    Ian Wickersham
    Insert
    splitTVA950-P2A-EGFP
    Use
    AAV and Cre/Lox
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    59332-plasmid-map-sequence-id-92028

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  3. pAAV-synP-FLEX-EGFP-B19G

    Plasmid
    #59333
    Purpose
    Expresses EGFP-P2A-B19G
    Depositor
    Ian Wickersham
    Insert
    EGFP-P2A-B19G
    Use
    AAV and Cre/Lox
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Synapsin-1
    Availability
    Academic Institutions and Nonprofits only
    59333-plasmid-map-sequence-id-92029

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  4. pAAV-CAG-tdTomato (codon diversified)

    Plasmid
    #59462
    Purpose
    AAV-mediated expression of tdTomato (codon diversified) under the CAG promoter
    Depositor
    Edward Boyden
    Has Service
    AAV PHP.S, AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    tdTomato
    Use
    AAV
    Tags
    Expression
    Mammalian
    Mutation
    codon diversified
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    59462-plasmid-map-sequence-id-423489

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  9. N-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58693
    Purpose
    Expresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    3xFlag, GyrA Nsplit Intein, and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    58693-plasmid-map-sequence-id-90684

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  10. C-Terminal Split Cas9 with GyrA intein

    Plasmid
    #58694
    Purpose
    Expresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    Humanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Csplit Intein and NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    58694-plasmid-map-sequence-id-90691

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  11. N-Terminal Split Cas9 D10A Nickase with GyrA intein

    Plasmid
    #58695
    Purpose
    Expresses N-terminus of D10A SpCas9 nickase domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 nickase production
    Depositor
    Gang Bao
    Article
    Fine et al Sci Rep. 2015 Jul 1;5:10777. doi: 10.103
    Insert
    D10A Nickase humanized S. pyogenes Cas9 with Gyra Nsplit Intein
    Use
    AAV and CRISPR
    Tags
    GyrA Nsplit Intein, HA Tag, and NLS
    Expression
    Mammalian
    Mutation
    D10A nickase converting mutation to SpCas9
    Promoter
    CBh
    Availability
    Academic Institutions and Nonprofits only
    58695-plasmid-map-sequence-id-90694

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Showing: 81 - 100 of 3757 results