pAAV-anti-CCL2(8E10) PAGER(TF)
(Plasmid #230002)

• Purpose: Expresses anti-CCL2 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)
• Depositing Lab: Alice Ting
• Publication: Kalogriopoulos et al Nature. 2024 Dec 4. doi: 10.1038/s41586-024-08282-3.

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Backbone

• Vector backbone: pAAV
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IL2SP-Aro6-8E10-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
• Species: Synthetic
• Mutation: V360A/R361A on KORD
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry