Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pSG5 EDD C2768A
(Plasmid #37192)


Item Catalog # Description Quantity Price (USD)
Plasmid 37192 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 4225
  • Total vector size (bp) 12652
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    EDD C2768A
  • Alt name
    UBR5 C2768A
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Changed Cysteine 2768 to Alanine; K503R (please see depositor comments below)
  • Entrez Gene
    UBR5 (a.k.a. DD5, EDD, EDD1, HYD)
  • Promoter SV40
  • Tags / Fusion Proteins
    • FLAG (N terminal on insert)
    • 6x His Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site RsrII (unknown if destroyed)
  • 3′ cloning site SfuI (unknown if destroyed)
  • 5′ sequencing primer T7 Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Amanda Russell, Cancer Research Program, Garvan Institute of Medical Research, NSW, Australia

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The depositing lab has noted that mutation K503R shouldn’t be a problem as it is outside the main functional domains. There does appear to be a relatively common SNP at K503 in Latino populations so this might be a genuine SNP-

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSG5 EDD C2768A was a gift from Darren Saunders & Charles Watts (Addgene plasmid # 37192 ; ; RRID:Addgene_37192)
  • For your References section:

    EDD, the human hyperplastic discs protein, has a role in progesterone receptor coactivation and potential involvement in DNA damage response. Henderson MJ, Russell AJ, Hird S, Munoz M, Clancy JL, Lehrbach GM, Calanni ST, Jans DA, Sutherland RL, Watts CK. J Biol Chem. 2002 Jul 19;277(29):26468-78. Epub 2002 May 13. 10.1074/jbc.M203527200 PubMed 12011095