Skip to main content

pCFD4-U6:1_U6:3tandemgRNAs Citations (125)

Originally described in: Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila.
Port F, Chen HM, Lee T, Bullock SL Proc Natl Acad Sci U S A. 2014 Jul 7. pii: 201405500.
PubMed Journal

Articles Citing pCFD4-U6:1_U6:3tandemgRNAs

Articles
The mystery of sleep function: current perspectives and future directions. Frank MG. Rev Neurosci. 2006;17(4):375-92. doi: 10.1515/revneuro.2006.17.4.375. PubMed
Cas9-based genome editing in Drosophila. Housden BE, Lin S, Perrimon N. Methods Enzymol. 2014;546:415-39. doi: 10.1016/B978-0-12-801185-0.00019-2. PubMed
Drosophila Lgr3 Couples Organ Growth with Maturation and Ensures Developmental Stability. Colombani J, Andersen DS, Boulan L, Boone E, Romero N, Virolle V, Texada M, Leopold P. Curr Biol. 2015 Oct 19;25(20):2723-9. doi: 10.1016/j.cub.2015.09.020. Epub 2015 Oct 1. PubMed
The postsynaptic t-SNARE Syntaxin 4 controls traffic of Neuroligin 1 and Synaptotagmin 4 to regulate retrograde signaling. Harris KP, Zhang YV, Piccioli ZD, Perrimon N, Littleton JT. Elife. 2016 May 25;5. pii: e13881. doi: 10.7554/eLife.13881. PubMed
Editing Transgenic DNA Components by Inducible Gene Replacement in Drosophila melanogaster. Lin CC, Potter CJ. Genetics. 2016 Jun 22. pii: genetics.116.191783. PubMed

Associated Plasmids

Rapid Screening for CRISPR-Directed Editing of the Drosophila Genome Using white Co-conversion. Ge DT, Tipping C, Brodsky MH, Zamore PD. G3 (Bethesda). 2016 Aug 26. pii: g3.116.032557. doi: 10.1534/g3.116.032557. PubMed

Associated Plasmids

Creating Heritable Mutations in Drosophila with CRISPR-Cas9. Port F, Bullock SL. Methods Mol Biol. 2016;1478:145-160. PubMed
The Hippo signalling pathway coordinates organ growth and limits developmental variability by controlling dilp8 expression. Boone E, Colombani J, Andersen DS, Leopold P. Nat Commun. 2016 Nov 22;7:13505. doi: 10.1038/ncomms13505. PubMed
Modeling Monogenic Human Nephrotic Syndrome in the Drosophila Garland Cell Nephrocyte. Hermle T, Braun DA, Helmstadter M, Huber TB, Hildebrandt F. J Am Soc Nephrol. 2017 May;28(5):1521-1533. doi: 10.1681/ASN.2016050517. Epub 2016 Dec 8. PubMed
Peptidoglycan sensing by octopaminergic neurons modulates Drosophila oviposition. Kurz CL, Charroux B, Chaduli D, Viallat-Lieutaud A, Royet J. Elife. 2017 Mar 7;6. doi: 10.7554/eLife.21937. PubMed
Neuronal cell fate specification by the molecular convergence of different spatio-temporal cues on a common initiator terminal selector gene. Stratmann J, Thor S. PLoS Genet. 2017 Apr 17;13(4):e1006729. doi: 10.1371/journal.pgen.1006729. eCollection 2017 Apr. PubMed
SLC46 Family Transporters Facilitate Cytosolic Innate Immune Recognition of Monomeric Peptidoglycans. Paik D, Monahan A, Caffrey DR, Elling R, Goldman WE, Silverman N. J Immunol. 2017 Jul 1;199(1):263-270. doi: 10.4049/jimmunol.1600409. Epub 2017 May 24. PubMed
A Peptidergic Circuit Links the Circadian Clock to Locomotor Activity. King AN, Barber AF, Smith AE, Dreyer AP, Sitaraman D, Nitabach MN, Cavanaugh DJ, Sehgal A. Curr Biol. 2017 Jul 10;27(13):1915-1927.e5. doi: 10.1016/j.cub.2017.05.089. Epub 2017 Jun 29. PubMed
Multiple P450s and Variation in Neuronal Genes Underpins the Response to the Insecticide Imidacloprid in a Population of Drosophila melanogaster. Denecke S, Fusetto R, Martelli F, Giang A, Battlay P, Fournier-Level A, O' Hair RA, Batterham P. Sci Rep. 2017 Sep 12;7(1):11338. doi: 10.1038/s41598-017-11092-5. PubMed
Opbp is a new architectural/insulator protein required for ribosomal gene expression. Zolotarev N, Maksimenko O, Kyrchanova O, Sokolinskaya E, Osadchiy I, Girardot C, Bonchuk A, Ciglar L, Furlong EEM, Georgiev P. Nucleic Acids Res. 2017 Dec 1;45(21):12285-12300. doi: 10.1093/nar/gkx840. PubMed
Describing the role of Drosophila melanogaster ABC transporters in insecticide biology using CRISPR-Cas9 knockouts. Denecke S, Fusetto R, Batterham P. Insect Biochem Mol Biol. 2017 Dec;91:1-9. doi: 10.1016/j.ibmb.2017.09.017. Epub 2017 Oct 20. PubMed
CRISPR/Cas9 and active genetics-based trans-species replacement of the endogenous Drosophila kni-L2 CRM reveals unexpected complexity. Xu XS, Gantz VM, Siomava N, Bier E. Elife. 2017 Dec 23;6. pii: 30281. doi: 10.7554/eLife.30281. PubMed
Cytosolic and Secreted Peptidoglycan-Degrading Enzymes in Drosophila Respectively Control Local and Systemic Immune Responses to Microbiota. Charroux B, Capo F, Kurz CL, Peslier S, Chaduli D, Viallat-Lieutaud A, Royet J. Cell Host Microbe. 2018 Feb 14;23(2):215-228.e4. doi: 10.1016/j.chom.2017.12.007. Epub 2018 Feb 1. PubMed
A Conserved Circadian Function for the Neurofibromatosis 1 Gene. Bai L, Lee Y, Hsu CT, Williams JA, Cavanaugh D, Zheng X, Stein C, Haynes P, Wang H, Gutmann DH, Sehgal A. Cell Rep. 2018 Mar 27;22(13):3416-3426. doi: 10.1016/j.celrep.2018.03.014. PubMed
A population of adult satellite-like cells in Drosophila is maintained through a switch in RNA-isoforms. Boukhatmi H, Bray S. Elife. 2018 Apr 9;7. pii: 35954. doi: 10.7554/eLife.35954. PubMed
Reducing resistance allele formation in CRISPR gene drive. Champer J, Liu J, Oh SY, Reeves R, Luthra A, Oakes N, Clark AG, Messer PW. Proc Natl Acad Sci U S A. 2018 May 22;115(21):5522-5527. doi: 10.1073/pnas.1720354115. Epub 2018 May 7. PubMed
gamma-TuRC Heterogeneity Revealed by Analysis of Mozart1. Tovey CA, Tubman CE, Hamrud E, Zhu Z, Dyas AE, Butterfield AN, Fyfe A, Johnson E, Conduit PT. Curr Biol. 2018 Jul 23;28(14):2314-2323.e6. doi: 10.1016/j.cub.2018.05.044. Epub 2018 Jul 5. PubMed
A Developmental Program Truncates Long Transcripts to Temporally Regulate Cell Signaling. Sandler JE, Irizarry J, Stepanik V, Dunipace L, Amrhein H, Stathopoulos A. Dev Cell. 2018 Dec 17;47(6):773-784.e6. doi: 10.1016/j.devcel.2018.11.019. PubMed
The apical protein Apnoia interacts with Crumbs to regulate tracheal growth and inflation. Skouloudaki K, Papadopoulos DK, Tomancak P, Knust E. PLoS Genet. 2019 Jan 15;15(1):e1007852. doi: 10.1371/journal.pgen.1007852. eCollection 2019 Jan. PubMed
Sperm-Leucylaminopeptidases are required for male fertility as structural components of mitochondrial paracrystalline material in Drosophila melanogaster sperm. Laurinyecz B, Vedelek V, Kovacs AL, Szilasi K, Lipinszki Z, Slezak C, Darula Z, Juhasz G, Sinka R. PLoS Genet. 2019 Feb 25;15(2):e1007987. doi: 10.1371/journal.pgen.1007987. eCollection 2019 Feb. PubMed
The molecular and cellular basis of olfactory response to tsetse fly attractants. Chahda JS, Soni N, Sun JS, Ebrahim SAM, Weiss BL, Carlson JR. PLoS Genet. 2019 Mar 15;15(3):e1008005. doi: 10.1371/journal.pgen.1008005. eCollection 2019 Mar. PubMed
A Cut/cohesin axis alters the chromatin landscape to facilitate neuroblast death. Arya R, Gyonjyan S, Harding K, Sarkissian T, Li Y, Zhou L, White K. Development. 2019 May 1;146(9). pii: dev.166603. doi: 10.1242/dev.166603. PubMed
Dual separable feedback systems govern firing rate homeostasis. Kulik Y, Jones R, Moughamian AJ, Whippen J, Davis GW. Elife. 2019 Apr 11;8. pii: 45717. doi: 10.7554/eLife.45717. PubMed
Comprehensive Genetic Characterization of Mitochondrial Ca(2+) Uniporter Components Reveals Their Different Physiological Requirements In Vivo. Tufi R, Gleeson TP, von Stockum S, Hewitt VL, Lee JJ, Terriente-Felix A, Sanchez-Martinez A, Ziviani E, Whitworth AJ. Cell Rep. 2019 Apr 30;27(5):1541-1550.e5. doi: 10.1016/j.celrep.2019.04.033. PubMed
Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila. Meltzer H, Marom E, Alyagor I, Mayseless O, Berkun V, Segal-Gilboa N, Unger T, Luginbuhl D, Schuldiner O. Nat Commun. 2019 May 8;10(1):2113. doi: 10.1038/s41467-019-10140-0. PubMed
Chemosensory sensilla of the Drosophila wing express a candidate ionotropic pheromone receptor. He Z, Luo Y, Shang X, Sun JS, Carlson JR. PLoS Biol. 2019 May 21;17(5):e2006619. doi: 10.1371/journal.pbio.2006619. eCollection 2019 May. PubMed
Daedalus and Gasz recruit Armitage to mitochondria, bringing piRNA precursors to the biogenesis machinery. Munafo M, Manelli V, Falconio FA, Sawle A, Kneuss E, Eastwood EL, Seah JWE, Czech B, Hannon GJ. Genes Dev. 2019 Jul 1;33(13-14):844-856. doi: 10.1101/gad.325662.119. Epub 2019 May 23. PubMed
piRNA-guided co-transcriptional silencing coopts nuclear export factors. Fabry MH, Ciabrelli F, Munafo M, Eastwood EL, Kneuss E, Falciatori I, Falconio FA, Hannon GJ, Czech B. Elife. 2019 Jun 20;8. pii: 47999. doi: 10.7554/eLife.47999. PubMed
Anterior CNS expansion driven by brain transcription factors. Curt JR, Yaghmaeian Salmani B, Thor S. Elife. 2019 Jul 4;8. pii: 45274. doi: 10.7554/eLife.45274. PubMed
Parcas is the predominant Rab11-GEF for rhodopsin transport in Drosophila photoreceptors. Otsuka Y, Satoh T, Nakayama N, Inaba R, Yamashita H, Satoh AK. J Cell Sci. 2019 Aug 7;132(15). pii: jcs.231431. doi: 10.1242/jcs.231431. PubMed
Multilevel regulation of the glass locus during Drosophila eye development. Fritsch C, Bernardo-Garcia FJ, Humberg TH, Mishra AK, Miellet S, Almeida S, Frochaux MV, Deplancke B, Huber A, Sprecher SG. PLoS Genet. 2019 Jul 12;15(7):e1008269. doi: 10.1371/journal.pgen.1008269. eCollection 2019 Jul. PubMed
Defects of mitochondrial RNA turnover lead to the accumulation of double-stranded RNA in vivo. Pajak A, Laine I, Clemente P, El-Fissi N, Schober FA, Maffezzini C, Calvo-Garrido J, Wibom R, Filograna R, Dhir A, Wedell A, Freyer C, Wredenberg A. PLoS Genet. 2019 Jul 31;15(7):e1008240. doi: 10.1371/journal.pgen.1008240. eCollection 2019 Jul. PubMed
Functional Comparison of Short and Long Isoforms of the TRF2 Protein in Drosophila melanogaster. Osadchiy IS, Georgiev PG, Maksimenko OG. Dokl Biochem Biophys. 2019 May;486(1):224-228. doi: 10.1134/S1607672919030165. Epub 2019 Jul 31. PubMed
Specialization of the Drosophila nuclear export family protein Nxf3 for piRNA precursor export. Kneuss E, Munafo M, Eastwood EL, Deumer US, Preall JB, Hannon GJ, Czech B. Genes Dev. 2019 Sep 1;33(17-18):1208-1220. doi: 10.1101/gad.328690.119. Epub 2019 Aug 15. PubMed
CRISPR/Cas9 Genome Editing to Study Nervous System Development in Drosophila. Fritsch C, Sprecher SG. Methods Mol Biol. 2020;2047:161-189. doi: 10.1007/978-1-4939-9732-9_10. PubMed
Drosulfakinin signaling in fruitless circuitry antagonizes P1 neurons to regulate sexual arousal in Drosophila. Wu S, Guo C, Zhao H, Sun M, Chen J, Han C, Peng Q, Qiao H, Peng P, Liu Y, Luo SD, Pan Y. Nat Commun. 2019 Oct 18;10(1):4770. doi: 10.1038/s41467-019-12758-6. PubMed
Robust olfactory responses in the absence of odorant binding proteins. Xiao S, Sun JS, Carlson JR. Elife. 2019 Oct 25;8. pii: 51040. doi: 10.7554/eLife.51040. PubMed
TBC1D8B Mutations Implicate RAB11-Dependent Vesicular Trafficking in the Pathogenesis of Nephrotic Syndrome. Kampf LL, Schneider R, Gerstner L, Thunauer R, Chen M, Helmstadter M, Amar A, Onuchic-Whitford AC, Loza Munarriz R, Berdeli A, Muller D, Schrezenmeier E, Budde K, Mane S, Laricchia KM, Rehm HL, MacArthur DG, Lifton RP, Walz G, Romer W, Bergmann C, Hildebrandt F, Hermle T. J Am Soc Nephrol. 2019 Dec;30(12):2338-2353. doi: 10.1681/ASN.2019040414. Epub 2019 Nov 15. PubMed
Coacting enhancers can have complementary functions within gene regulatory networks and promote canalization. Dunipace L, Akos Z, Stathopoulos A. PLoS Genet. 2019 Dec 12;15(12):e1008525. doi: 10.1371/journal.pgen.1008525. eCollection 2019 Dec. PubMed
Dopamine modulates the optomotor response to unreliable visual stimuli in Drosophila melanogaster. Akiba M, Sugimoto K, Aoki R, Murakami R, Miyashita T, Hashimoto R, Hiranuma A, Yamauchi J, Ueno T, Morimoto T. Eur J Neurosci. 2020 Feb;51(3):822-839. doi: 10.1111/ejn.14648. Epub 2019 Dec 30. PubMed
Molecular Logic and Evolution of Bitter Taste in Drosophila. Dweck HKM, Carlson JR. Curr Biol. 2020 Jan 6;30(1):17-30.e3. doi: 10.1016/j.cub.2019.11.005. Epub 2019 Dec 12. PubMed
Prickle isoforms determine handedness of helical morphogenesis. Cho B, Song S, Axelrod JD. Elife. 2020 Jan 14;9:e51456. doi: 10.7554/eLife.51456. PubMed
A transcomplementing gene drive provides a flexible platform for laboratory investigation and potential field deployment. Lopez Del Amo V, Bishop AL, Sanchez C HM, Bennett JB, Feng X, Marshall JM, Bier E, Gantz VM. Nat Commun. 2020 Jan 17;11(1):352. doi: 10.1038/s41467-019-13977-7. PubMed
A fly model establishes distinct mechanisms for synthetic CRISPR/Cas9 sex distorters. Fasulo B, Meccariello A, Morgan M, Borufka C, Papathanos PA, Windbichler N. PLoS Genet. 2020 Mar 13;16(3):e1008647. doi: 10.1371/journal.pgen.1008647. eCollection 2020 Mar. PubMed
Drosophila Synaptotagmin 7 negatively regulates synaptic vesicle release and replenishment in a dosage-dependent manner. Guan Z, Quinones-Frias MC, Akbergenova Y, Littleton JT. Elife. 2020 Apr 28;9:e55443. doi: 10.7554/eLife.55443. PubMed
Anandamide Metabolites Protect against Seizures through the TRP Channel Water Witch in Drosophila melanogaster. Jacobs JA, Sehgal A. Cell Rep. 2020 Jun 2;31(9):107710. doi: 10.1016/j.celrep.2020.107710. PubMed
Evolutionary trade-offs of insecticide resistance - The fitness costs associated with target-site mutations in the nAChR of Drosophila melanogaster. Homem RA, Buttery B, Richardson E, Tan Y, Field LM, Williamson MS, Emyr Davies TG. Mol Ecol. 2020 Jul;29(14):2661-2675. doi: 10.1111/mec.15503. Epub 2020 Jun 22. PubMed
Intrinsic control of muscle attachment sites matching. Carayon A, Bataille L, Lebreton G, Dubois L, Pelletier A, Carrier Y, Wystrach A, Vincent A, Frendo JL. Elife. 2020 Jul 24;9. pii: 57547. doi: 10.7554/eLife.57547. PubMed
A Drosophila model of Friedreich ataxia with CRISPR/Cas9 insertion of GAA repeats in the frataxin gene reveals in vivo protection by N-acetyl cysteine. Russi M, Martin E, D'Autreaux B, Tixier L, Tricoire H, Monnier V. Hum Mol Genet. 2020 Oct 10;29(17):2831-2844. doi: 10.1093/hmg/ddaa170. PubMed
Enteric neurons increase maternal food intake during reproduction. Hadjieconomou D, King G, Gaspar P, Mineo A, Blackie L, Ameku T, Studd C, de Mendoza A, Diao F, White BH, Brown AEX, Placais PY, Preat T, Miguel-Aliaga I. Nature. 2020 Nov;587(7834):455-459. doi: 10.1038/s41586-020-2866-8. Epub 2020 Oct 28. PubMed
Modulation of Yorkie activity by alternative splicing is required for developmental stability. Srivastava D, de Toledo M, Manchon L, Tazi J, Juge F. EMBO J. 2021 Feb 1;40(3):e104895. doi: 10.15252/embj.2020104895. Epub 2020 Dec 15. PubMed
fruitless tunes functional flexibility of courtship circuitry during development. Chen J, Jin S, Chen D, Cao J, Ji X, Peng Q, Pan Y. Elife. 2021 Jan 19;10. pii: 59224. doi: 10.7554/eLife.59224. PubMed
Ribosomopathy-associated mutations cause proteotoxic stress that is alleviated by TOR inhibition. Recasens-Alvarez C, Alexandre C, Kirkpatrick J, Nojima H, Huels DJ, Snijders AP, Vincent JP. Nat Cell Biol. 2021 Feb;23(2):127-135. doi: 10.1038/s41556-020-00626-1. Epub 2021 Jan 25. PubMed
Rpd3/CoRest-mediated activity-dependent transcription regulates the flexibility in memory updating in Drosophila. Takakura M, Nakagawa R, Ota T, Kimura Y, Ng MY, Alia AG, Okuno H, Hirano Y. Nat Commun. 2021 Jan 27;12(1):628. doi: 10.1038/s41467-021-20898-x. PubMed
Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation. Dobramysl U, Jarsch IK, Inoue Y, Shimo H, Richier B, Gadsby JR, Mason J, Szalapak A, Ioannou PS, Correia GP, Walrant A, Butler R, Hannezo E, Simons BD, Gallop JL. J Cell Biol. 2021 Apr 5;220(4). pii: 211897. doi: 10.1083/jcb.202003052. PubMed
Axonal chemokine-like Orion induces astrocyte infiltration and engulfment during mushroom body neuronal remodeling. Boulanger A, Thinat C, Zuchner S, Fradkin LG, Lortat-Jacob H, Dura JM. Nat Commun. 2021 Mar 23;12(1):1849. doi: 10.1038/s41467-021-22054-x. PubMed
Drosophila TNFRs Grindelwald and Wengen bind Eiger with different affinities and promote distinct cellular functions. Palmerini V, Monzani S, Laurichesse Q, Loudhaief R, Mari S, Cecatiello V, Olieric V, Pasqualato S, Colombani J, Andersen DS, Mapelli M. Nat Commun. 2021 Apr 6;12(1):2070. doi: 10.1038/s41467-021-22080-9. PubMed
RNA kinase CLP1/Cbc regulates meiosis initiation in spermatogenesis. Wu J, Li X, Gao Z, Pang L, Liu X, Huang X, Wang Y, Wang Z. Hum Mol Genet. 2021 Aug 12;30(17):1569-1578. doi: 10.1093/hmg/ddab107. PubMed
Sight of parasitoid wasps accelerates sexual behavior and upregulates a micropeptide gene in Drosophila. Ebrahim SAM, Talross GJS, Carlson JR. Nat Commun. 2021 Apr 27;12(1):2453. doi: 10.1038/s41467-021-22712-0. PubMed
Engineered reproductively isolated species drive reversible population replacement. Buchman A, Shriner I, Yang T, Liu J, Antoshechkin I, Marshall JM, Perry MW, Akbari OS. Nat Commun. 2021 Jun 2;12(1):3281. doi: 10.1038/s41467-021-23531-z. PubMed

Associated Plasmids

Short and long sleeping mutants reveal links between sleep and macroautophagy. Bedont JL, Toda H, Shi M, Park CH, Quake C, Stein C, Kolesnik A, Sehgal A. Elife. 2021 Jun 4;10. pii: 64140. doi: 10.7554/eLife.64140. PubMed
CRISPR/Cas9-engineered Drosophila knock-in models to study VCP diseases. Wall JM, Basu A, Zunica ERM, Dubuisson OS, Pergola K, Broussard JP, Kirwan JP, Axelrod CL, Johnson AE. Dis Model Mech. 2021 Jul 1;14(7). pii: 269247. doi: 10.1242/dmm.048603. Epub 2021 Jul 12. PubMed
An actin-related protein that is most highly expressed in Drosophila testes is critical for embryonic development. Schroeder CM, Tomlin SA, Mejia Natividad I, Valenzuela JR, Young JM, Malik HS. Elife. 2021 Jul 20;10:e71279. doi: 10.7554/eLife.71279. PubMed
The Nrf2-Keap1 pathway is activated by steroid hormone signaling to govern neuronal remodeling. Chew LY, Zhang H, He J, Yu F. Cell Rep. 2021 Aug 3;36(5):109466. doi: 10.1016/j.celrep.2021.109466. PubMed
The fushi tarazu zebra element is not required for Drosophila viability or fertility. Graham PL, Fischer MD, Giri A, Pick L. G3 (Bethesda). 2021 Oct 19;11(11). pii: 6358135. doi: 10.1093/g3journal/jkab300. PubMed
The Role of Drosophila CtIP in Homology-Directed Repair of DNA Double-Strand Breaks. Yannuzzi I, Butler MA, Fernandez J, LaRocque JR. Genes (Basel). 2021 Sep 16;12(9). pii: genes12091430. doi: 10.3390/genes12091430. PubMed
The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity. Sauvola CW, Akbergenova Y, Cunningham KL, Aponte-Santiago NA, Littleton JT. Elife. 2021 Oct 29;10. pii: 72841. doi: 10.7554/eLife.72841. PubMed
The expression of Drosophila melanogaster Hox gene Ultrabithorax is not overtly regulated by the intronic long noncoding RNA lncRNA:PS4 in a wild-type genetic background. Hermann A, Kosman D, McGinnis W, Tour E. G3 (Bethesda). 2022 Jan 4;12(1). pii: 6428535. doi: 10.1093/g3journal/jkab374. PubMed
Loss of the Dbeta1 nicotinic acetylcholine receptor subunit disrupts bursicon-driven wing expansion and diminishes adult viability in Drosophila melanogaster. Christesen D, Yang YT, Chen W, Batterham P, Perry T. Genetics. 2021 Aug 26;219(1):iyab112. doi: 10.1093/genetics/iyab112. PubMed
The Piragua Gene Is not Essential for Drosophila Development. Sokolov VV, Georgiev PG, Kyrchanova OV. Dokl Biol Sci. 2021 Nov;501(1):197-200. doi: 10.1134/S0012496621060089. Epub 2021 Dec 28. PubMed
brinker levels regulated by a promoter proximal element support germ cell homeostasis. Dunipace L, Newcomb S, Stathopoulos A. Development. 2022 Feb 1;149(3). pii: 274023. doi: 10.1242/dev.199890. Epub 2022 Feb 4. PubMed
Prickle isoform participation in distinct polarization events in the Drosophila eye. Cho B, Song S, Wan JY, Axelrod JD. PLoS One. 2022 Feb 11;17(2):e0262328. doi: 10.1371/journal.pone.0262328. eCollection 2022. PubMed
Regulation of Drosophila courtship behavior by the Tlx/tailless-like nuclear receptor, dissatisfaction. Duckhorn JC, Cande J, Metkus MC, Song H, Altamirano S, Stern DL, Shirangi TR. Curr Biol. 2022 Apr 25;32(8):1703-1714.e3. doi: 10.1016/j.cub.2022.02.031. Epub 2022 Mar 3. PubMed
Re-evaluating the actin-dependence of spectraplakin functions during axon growth and maintenance. Qu Y, Alves-Silva J, Gupta K, Hahn I, Parkin J, Sanchez-Soriano N, Prokop A. Dev Neurobiol. 2022 May;82(4):288-307. doi: 10.1002/dneu.22873. Epub 2022 Apr 22. PubMed
Fluorescent labeling of genomic loci in Drosophila imaginal discs with heterologous DNA-binding proteins. Delker RK, Munce RH, Hu M, Mann RS. Cell Rep Methods. 2022 Mar 9;2(3):100175. doi: 10.1016/j.crmeth.2022.100175. eCollection 2022 Mar 28. PubMed
The vestigial Quadrant Enhancer is dispensable for pattern formation and development of the Drosophila wing. Farfan-Pira KJ, Martinez-Cuevas TI, Reyes R, Evans TA, Nahmad M. MicroPubl Biol. 2022 Jun 13;2022:10.17912/micropub.biology.000585. doi: 10.17912/micropub.biology.000585. eCollection 2022. PubMed
Drosophila MESR4 Gene Ensures Germline Stem Cell Differentiation by Promoting the Transcription of bag of marbles. Szarka-Kovacs AB, Takacs Z, Bence M, Erdelyi M, Jankovics F. Cells. 2022 Jun 28;11(13). pii: cells11132056. doi: 10.3390/cells11132056. PubMed
Different transcriptional responses by the CRISPRa system in distinct types of heterochromatin in Drosophila melanogaster. Ortega-Yanez A, Cruz-Ruiz S, Vazquez M, Zurita M. Sci Rep. 2022 Jul 9;12(1):11702. doi: 10.1038/s41598-022-15944-7. PubMed
Regulation of presynaptic Ca(2+) channel abundance at active zones through a balance of delivery and turnover. Cunningham KL, Sauvola CW, Tavana S, Littleton JT. Elife. 2022 Jul 14;11:e78648. doi: 10.7554/eLife.78648. PubMed
Function of Transient Receptor Potential-Like Channel in Insect Egg Laying. Zhang Y, Zhang YJ, Guo D, Wang LX, Niu CD, Wu SF, Zhang YV, Gao CF. Front Mol Neurosci. 2022 Jun 30;15:823563. doi: 10.3389/fnmol.2022.823563. eCollection 2022. PubMed
A neurogenetic mechanism of experience-dependent suppression of aggression. Ishii K, Cortese M, Leng X, Shokhirev MN, Asahina K. Sci Adv. 2022 Sep 9;8(36):eabg3203. doi: 10.1126/sciadv.abg3203. Epub 2022 Sep 7. PubMed
ANGEL2 phosphatase activity is required for non-canonical mitochondrial RNA processing. Clemente P, Calvo-Garrido J, Pearce SF, Schober FA, Shigematsu M, Siira SJ, Laine I, Spahr H, Steinmetzger C, Petzold K, Kirino Y, Wibom R, Rackham O, Filipovska A, Rorbach J, Freyer C, Wredenberg A. Nat Commun. 2022 Sep 30;13(1):5750. doi: 10.1038/s41467-022-33368-9. PubMed
The Drosophila ZAD zinc finger protein Kipferl guides Rhino to piRNA clusters. Baumgartner L, Handler D, Platzer SW, Yu C, Duchek P, Brennecke J. Elife. 2022 Oct 4;11:e80067. doi: 10.7554/eLife.80067. PubMed
Expansion and loss of sperm nuclear basic protein genes in Drosophila correspond with genetic conflicts between sex chromosomes. Chang CH, Mejia Natividad I, Malik HS. Elife. 2023 Feb 10;12:e85249. doi: 10.7554/eLife.85249. PubMed
SREBP modulates the NADP(+)/NADPH cycle to control night sleep in Drosophila. Mariano V, Kanellopoulos AK, Aiello G, Lo AC, Legius E, Achsel T, Bagni C. Nat Commun. 2023 Feb 20;14(1):763. doi: 10.1038/s41467-022-35577-8. PubMed
Evolutionary diversification reveals distinct somatic versus germline cytoskeletal functions of the Arp2 branched actin nucleator protein. Stromberg KA, Spain T, Tomlin SA, Amarillo KD, Schroeder CM. bioRxiv. 2023 Feb 27:2023.02.25.530036. doi: 10.1101/2023.02.25.530036. Preprint. PubMed
A cis-regulatory sequence of the selector gene vestigial drives the evolution of wing scaling in Drosophila species. Farfan-Pira KJ, Martinez-Cuevas TI, Evans TA, Nahmad M. J Exp Biol. 2023 May 15;226(10):jeb244692. doi: 10.1242/jeb.244692. Epub 2023 May 18. PubMed
The Drosophila chemokine-like Orion bridges phosphatidylserine and Draper in phagocytosis of neurons. Ji H, Wang B, Shen Y, Labib D, Lei J, Chen X, Sapar M, Boulanger A, Dura JM, Han C. Proc Natl Acad Sci U S A. 2023 Jun 13;120(24):e2303392120. doi: 10.1073/pnas.2303392120. Epub 2023 Jun 5. PubMed
Reduced histone gene copy number disrupts Drosophila Polycomb function. McPherson JE, Grossmann LC, Salzler HR, Armstrong RL, Kwon E, Matera AG, McKay DJ, Duronio RJ. Genetics. 2023 Aug 9;224(4):iyad106. doi: 10.1093/genetics/iyad106. PubMed
A phylogenetic profiling approach identifies novel ciliogenesis genes in Drosophila and C. elegans. Dobbelaere J, Su TY, Erdi B, Schleiffer A, Dammermann A. EMBO J. 2023 Aug 15;42(16):e113616. doi: 10.15252/embj.2023113616. Epub 2023 Jun 15. PubMed
G3'MTMD3 in the insect GABA receptor subunit, RDL, confers resistance to broflanilide and fluralaner. Zhang Y, Huang Q, Sheng C, Liu G, Zhang K, Jia Z, Tang T, Mao X, Jones AK, Han Z, Zhao C. PLoS Genet. 2023 Jun 29;19(6):e1010814. doi: 10.1371/journal.pgen.1010814. eCollection 2023 Jun. PubMed
CRISPR/Cas9-mediated knock-in in ebony gene using a PCR product donor template in Drosophila. Bui KC, Kamiyama D. Gene Genome Ed. 2023 Jun;5:100025. doi: 10.1016/j.ggedit.2023.100025. Epub 2023 Apr 21. PubMed
CRISPR/Cas9 Essential Gene Editing in Drosophila. Osadchiy IS, Kamalyan SO, Tumashova KY, Georgiev PG, Maksimenko OG. Acta Naturae. 2023 Apr-Jun;15(2):70-74. doi: 10.32607/actanaturae.11874. PubMed
Epithelial Ca(2+) waves triggered by enteric neurons heal the gut. Petsakou A, Liu Y, Liu Y, Comjean A, Hu Y, Perrimon N. bioRxiv [Preprint]. 2023 Aug 15:2023.08.14.553227. doi: 10.1101/2023.08.14.553227. PubMed
Cholinergic neurons trigger epithelial Ca(2+) currents to heal the gut. Petsakou A, Liu Y, Liu Y, Comjean A, Hu Y, Perrimon N. Nature. 2023 Nov;623(7985):122-131. doi: 10.1038/s41586-023-06627-y. Epub 2023 Sep 18. PubMed
Molecular and functional characterization of the Drosophila melanogaster conserved smORFome. Bosch JA, Keith N, Escobedo F, Fisher WW, LaGraff JT, Rabasco J, Wan KH, Weiszmann R, Hu Y, Kondo S, Brown JB, Perrimon N, Celniker SE. Cell Rep. 2023 Nov 28;42(11):113311. doi: 10.1016/j.celrep.2023.113311. Epub 2023 Oct 26. PubMed
PIGB maintains nuclear lamina organization in skeletal muscle of Drosophila. Yamamoto-Hino M, Ariura M, Tanaka M, Iwasaki YW, Kawaguchi K, Shimamoto Y, Goto S. J Cell Biol. 2024 Feb 5;223(2):e202301062. doi: 10.1083/jcb.202301062. Epub 2024 Jan 23. PubMed
Timeless noncoding DNA contains cell-type preferential enhancers important for proper Drosophila circadian regulation. Ma D, Ojha P, Yu AD, Araujo MS, Luo W, Keefer E, Diaz MM, Wu M, Joiner WJ, Abruzzi KC, Rosbash M. Proc Natl Acad Sci U S A. 2024 Apr 9;121(15):e2321338121. doi: 10.1073/pnas.2321338121. Epub 2024 Apr 3. PubMed
Inheritance of H3K9 methylation regulates genome architecture in Drosophila early embryos. Atinbayeva N, Valent I, Zenk F, Loeser E, Rauer M, Herur S, Quarato P, Pyrowolakis G, Gomez-Auli A, Mittler G, Cecere G, Erhardt S, Tiana G, Zhan Y, Iovino N. EMBO J. 2024 Jul;43(13):2685-2714. doi: 10.1038/s44318-024-00127-z. Epub 2024 Jun 3. PubMed
Drosophila Robo3 guides longitudinal axons partially independently of its cytodomain. Agcaoili J, Evans TA. MicroPubl Biol. 2024 Jun 1;2024:10.17912/micropub.biology.001228. doi: 10.17912/micropub.biology.001228. eCollection 2024. PubMed
Redesigning the Drosophila histone gene cluster: an improved genetic platform for spatiotemporal manipulation of histone function. Crain AT, Nevil M, Leatham-Jensen MP, Reeves KB, Matera AG, McKay DJ, Duronio RJ. Genetics. 2024 Sep 4;228(1):iyae117. doi: 10.1093/genetics/iyae117. PubMed
Stem-loop and circle-loop TADs generated by directional pairing of boundary elements have distinct physical and regulatory properties. Ke W, Fujioka M, Schedl P, Jaynes JB. Elife. 2024 Aug 7;13:RP94114. doi: 10.7554/eLife.94114. PubMed
A candidate loss-of-function variant in SGIP1 causes synaptic dysfunction and recessive parkinsonism. Decet M, Scott P, Kuenen S, Meftah D, Swerts J, Calatayud C, Gallego SF, Kaempf N, Nachman E, Praschberger R, Schoovaerts N, Tang CC, Eidelberg D, Al Adawi S, Al Asmi A, Nandhagopal R, Verstreken P. Cell Rep Med. 2024 Oct 15;5(10):101749. doi: 10.1016/j.xcrm.2024.101749. Epub 2024 Sep 26. PubMed
The Drosophila maternal-effect gene abnormal oocyte (ao) does not repress histone gene expression. Takenaka R, Simmerman SM, Schmidt CA, Albanese EH, Rieder LE, Malik HS. bioRxiv [Preprint]. 2024 Sep 18:2024.09.17.613536. doi: 10.1101/2024.09.17.613536. PubMed
Food hardness preference reveals multisensory contributions of fly larval gustatory organs in behaviour and physiology. Komarov N, Fritsch C, Maier GL, Bues J, Biocanin M, Avalos CB, Dodero A, Kwon JY, Deplancke B, Sprecher SG. PLoS Biol. 2025 Jan 30;23(1):e3002730. doi: 10.1371/journal.pbio.3002730. eCollection 2025 Jan. PubMed
A hormone-to-neuropeptide pathway inhibits sexual receptivity in immature Drosophila females. Chen J, Zhu P, Jin S, Zhang Z, Jiang S, Li S, Liu S, Peng Q, Pan Y. Proc Natl Acad Sci U S A. 2025 Feb 25;122(8):e2418481122. doi: 10.1073/pnas.2418481122. Epub 2025 Feb 21. PubMed
Pcf11/Spt5 condensates stall RNA polymerase II to facilitate termination and piRNA-guided heterochromatin formation. Liu W, Deng L, Wang M, Liu X, Ouyang X, Wang Y, Miao N, Luo X, Wu X, Lu X, Xv X, Zhang T, Li Y, Ji J, Qiao Z, Wang S, Guan L, Li D, Dang Y, Liu C, Li W, Zhang Y, Wang Z, Chen FX, Chen C, Lin C, Goh WSS, Zhou W, Luo Z, Gao P, Li P, Yu Y. Mol Cell. 2025 Mar 6;85(5):929-947.e10. doi: 10.1016/j.molcel.2025.01.023. Epub 2025 Feb 26. PubMed
Adapting Next-Generation Sequencing to in Process CRISPR-Cas9 Genome Editing of Recombinant AcMNPV Vectors: From Shotgun to Tiled-Amplicon Sequencing. Chakraborty M, Nielsen L, Nash D, Nissimov JI, Charles TC, Aucoin MG. Viruses. 2025 Mar 18;17(3):437. doi: 10.3390/v17030437. PubMed
The proximal enhancer of the snail gene mediates negative autoregulatory feedback in Drosophila melanogaster. Dunipace L, McGehee JM, Irizarry J, Stathopoulos A. Genetics. 2025 Jun 4;230(2):iyaf058. doi: 10.1093/genetics/iyaf058. PubMed
Synaptic deregulation of cholinergic projection neurons causes olfactory dysfunction across five fly Parkinsonism models. Pech U, Janssens J, Schoovaerts N, Kuenen S, Calatayud Aristoy C, Gallego SF, Makhzami S, Hulselmans GJ, Poovathingal S, Davie K, Bademosi AT, Swerts J, Vilain S, Aerts S, Verstreken P. Elife. 2025 Apr 3;13:RP98348. doi: 10.7554/eLife.98348. PubMed
Drosophila architectural proteins M1BP and Opbp cooperatively form the active promoter of a ribosomal protein gene. Osadchiy I, Umnova A, Pokholkova GV, Golovnin A, Gvozdev VA, Zhimulev IF, Georgiev P, Maksimenko O. Epigenetics Chromatin. 2025 Apr 16;18(1):20. doi: 10.1186/s13072-025-00584-8. PubMed
A library of lineage-specific driver lines connects developing neuronal circuits to behavior in the Drosophila ventral nerve cord. Soffers JHM, Beck E, Sytkowski DJ, Maughan ME, Devarakonda D, Zhu Y, Wilson BA, Chen YD, Erclik T, Truman JW, Skeath JB, Lacin H. Elife. 2025 Jun 10;14:RP106042. doi: 10.7554/eLife.106042. PubMed
Baculovirus Variant Detection from Transient CRISPR-Cas9-Mediated Disruption of gp64 at Different Gene Locations. Chakraborty M, Nielsen L, Nash D, Bruder MR, Nissimov JI, Charles TC, Aucoin MG. Int J Mol Sci. 2025 Jun 17;26(12):5805. doi: 10.3390/ijms26125805. PubMed
A temporally restricted function of the dopamine receptor Dop1R2 during memory formation. Kaldun JC, Calia E, Bangalore Mukunda GC, Fritsch C, Komarov N, Sprecher SG. Elife. 2025 Jul 9;13:RP99368. doi: 10.7554/eLife.99368. PubMed
Cytoplasmic localization of the mRNA encoding actin regulator, Serendipity-alpha, promotes adherens junction assembly and nuclear repositioning. Mahadevan T, Figard LR, Seede H, Sehgal P, Geng Y, McDonald K, Golding I, Sokac AM. bioRxiv [Preprint]. 2025 Jul 31:2025.07.29.667534. doi: 10.1101/2025.07.29.667534. PubMed
Multiple cis-regulatory modules ensure robust tup/islet1 function in dorsal muscle identity specification. Pelletier A, Carayon A, Carrier Y, Sengenes C, Dubois L, Frendo JL. Skelet Muscle. 2025 Aug 25;15(1):23. doi: 10.1186/s13395-025-00392-4. PubMed
Defining the roles of the Integrator, NEXT, and nuclear exosome complexes in Drosophila oogenesis. Lee Y, Biasini A, Tipping C, Hong SH, Zamore PD. Cell Rep. 2025 Oct 28;44(10):116329. doi: 10.1016/j.celrep.2025.116329. Epub 2025 Sep 23. PubMed
Fer3 is uniquely expressed in NotchOFF hemilineages, where it promotes interneuron identity. Drake KC, Lai SL, Doe CQ. Development. 2025 Nov 15;152(22):dev205118. doi: 10.1242/dev.205118. Epub 2025 Nov 14. PubMed
Peculiarities of the e(y)2 Gene Evolution in Deuterostomes and Drosophilinae. Vorontsova J, Belova E, Khrustaleva A, Umnova A, Arkova O, Boyko K, Nikolaeva A, Maksimenko O, Bonchuk A, Georgiev P, Cherezov R. Int J Mol Sci. 2025 Nov 3;26(21):10705. doi: 10.3390/ijms262110705. PubMed
A genetic circuit that extends the useful range of a BMP morphogen arose alongside insect wing evolution. Huang A, Cocconi L, Nicholls-Mindlin B, Alexandre C, Salbreux G, Vincent JP. Curr Biol. 2025 Dec 1;35(23):5840-5852.e15. doi: 10.1016/j.cub.2025.10.059. Epub 2025 Nov 17. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.