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pKLV-U6gRNA(BbsI)-PGKpuro2ABFP Citations (51)

Plasmid Article: Genome-wide recessive genetic screening in mammalian cells with a lentiviral CRISPR-guide RNA library.
Koike-Yusa H, Li Y, Tan EP, Velasco-Herrera Mdel C, Yusa K Nat Biotechnol. 2014 Mar;32(3):267-73. doi: 10.1038/nbt.2800. Epub 2013 Dec 23.
PubMed Journal

Articles Citing pKLV-U6gRNA(BbsI)-PGKpuro2ABFP

Articles
Simultaneous live imaging of the transcription and nuclear position of specific genes. Ochiai H, Sugawara T, Yamamoto T. Nucleic Acids Res. 2015 Jun 19. pii: gkv624. PubMed

Associated Plasmids

CRISPR/Cas9 System as an Agent for Eliminating Polyomavirus JC Infection. Wollebo HS, Bellizzi A, Kaminski R, Hu W, White MK, Khalili K. PLoS One. 2015 Sep 11;10(9):e0136046. doi: 10.1371/journal.pone.0136046. eCollection 2015. PubMed
AMPK Protects Leukemia-Initiating Cells in Myeloid Leukemias from Metabolic Stress in the Bone Marrow. Saito Y, Chapple RH, Lin A, Kitano A, Nakada D. Cell Stem Cell. 2015 Sep 29. pii: S1934-5909(15)00374-4. doi: 10.1016/j.stem.2015.08.019. PubMed

Associated Plasmids

CRISPR-CAS9 D10A nickase target-specific fluorescent labeling of double strand DNA for whole genome mapping and structural variation analysis. McCaffrey J, Sibert J, Zhang B, Zhang Y, Hu W, Riethman H, Xiao M. Nucleic Acids Res. 2016 Jan 29;44(2):e11. doi: 10.1093/nar/gkv878. Epub 2015 Oct 19. PubMed
CRISPR/gRNA-directed synergistic activation mediator (SAM) induces specific, persistent and robust reactivation of the HIV-1 latent reservoirs. Zhang Y, Yin C, Zhang T, Li F, Yang W, Kaminski R, Fagan PR, Putatunda R, Young WB, Khalili K, Hu W. Sci Rep. 2015 Nov 5;5:16277. doi: 10.1038/srep16277. PubMed
Functional screening of guide RNAs targeting the regulatory and structural HIV-1 viral genome for a cure of AIDS. Yin C, Zhang T, Li F, Yang F, Putatunda R, Young WB, Khalili K, Hu W, Zhang Y. AIDS. 2016 May 15;30(8):1163-74. doi: 10.1097/QAD.0000000000001079. PubMed
Unlinking an lncRNA from Its Associated cis Element. Paralkar VR, Taborda CC, Huang P, Yao Y, Kossenkov AV, Prasad R, Luan J, Davies JO, Hughes JR, Hardison RC, Blobel GA, Weiss MJ. Mol Cell. 2016 Apr 7;62(1):104-10. doi: 10.1016/j.molcel.2016.02.029. Epub 2016 Mar 31. PubMed
A Genome-wide CRISPR Screen Identifies CDC25A as a Determinant of Sensitivity to ATR Inhibitors. Ruiz S, Mayor-Ruiz C, Lafarga V, Murga M, Vega-Sendino M, Ortega S, Fernandez-Capetillo O. Mol Cell. 2016 Apr 21;62(2):307-313. doi: 10.1016/j.molcel.2016.03.006. Epub 2016 Apr 7. PubMed
Efficient CRISPR-mediated mutagenesis in primary immune cells using CrispRGold and a C57BL/6 Cas9 transgenic mouse line. Chu VT, Graf R, Wirtz T, Weber T, Favret J, Li X, Petsch K, Tran NT, Sieweke MH, Berek C, Kuhn R, Rajewsky K. Proc Natl Acad Sci U S A. 2016 Nov 1;113(44):12514-12519. Epub 2016 Oct 11. PubMed

Associated Plasmids

A Precision Medicine Approach to the Rescue of Function on Malignant Calmodulinopathic Long-QT Syndrome. Limpitikul WB, Dick IE, Tester DJ, Boczek NJ, Limphong P, Yang W, Choi MH, Babich J, DiSilvestre D, Kanter RJ, Tomaselli GF, Ackerman MJ, Yue DT. Circ Res. 2017 Jan 6;120(1):39-48. doi: 10.1161/CIRCRESAHA.116.309283. Epub 2016 Oct 20. PubMed
cis-Regulatory Circuits Regulating NEK6 Kinase Overexpression in Transformed B Cells Are Super-Enhancer Independent. Huang Y, Koues OI, Zhao JY, Liu R, Pyfrom SC, Payton JE, Oltz EM. Cell Rep. 2017 Mar 21;18(12):2918-2931. doi: 10.1016/j.celrep.2017.02.067. PubMed
Modulation of Human Leukocyte Antigen-C by Human Cytomegalovirus Stimulates KIR2DS1 Recognition by Natural Killer Cells. van der Ploeg K, Chang C, Ivarsson MA, Moffett A, Wills MR, Trowsdale J. Front Immunol. 2017 Mar 29;8:298. doi: 10.3389/fimmu.2017.00298. eCollection 2017. PubMed
Hyperactivation of HUSH complex function by Charcot-Marie-Tooth disease mutation in MORC2. Tchasovnikarova IA, Timms RT, Douse CH, Roberts RC, Dougan G, Kingston RE, Modis Y, Lehner PJ. Nat Genet. 2017 Jul;49(7):1035-1044. doi: 10.1038/ng.3878. Epub 2017 Jun 5. PubMed
alpha-ketoglutarate orchestrates macrophage activation through metabolic and epigenetic reprogramming. Liu PS, Wang H, Li X, Chao T, Teav T, Christen S, Di Conza G, Cheng WC, Chou CH, Vavakova M, Muret C, Debackere K, Mazzone M, Huang HD, Fendt SM, Ivanisevic J, Ho PC. Nat Immunol. 2017 Sep;18(9):985-994. doi: 10.1038/ni.3796. Epub 2017 Jul 17. PubMed
Trap(Seq): An RNA Sequencing-Based Pipeline for the Identification of Gene-Trap Insertions in Mammalian Cells. Mayor-Ruiz C, Dominguez O, Fernandez-Capetillo O. J Mol Biol. 2017 Sep 1;429(18):2780-2789. doi: 10.1016/j.jmb.2017.07.020. Epub 2017 Aug 4. PubMed
CMTM6 maintains the expression of PD-L1 and regulates anti-tumour immunity. Burr ML, Sparbier CE, Chan YC, Williamson JC, Woods K, Beavis PA, Lam EYN, Henderson MA, Bell CC, Stolzenburg S, Gilan O, Bloor S, Noori T, Morgens DW, Bassik MC, Neeson PJ, Behren A, Darcy PK, Dawson SJ, Voskoboinik I, Trapani JA, Cebon J, Lehner PJ, Dawson MA. Nature. 2017 Sep 7;549(7670):101-105. doi: 10.1038/nature23643. Epub 2017 Aug 16. PubMed
ENCoRE: an efficient software for CRISPR screens identifies new players in extrinsic apoptosis. Trumbach D, Pfeiffer S, Poppe M, Scherb H, Doll S, Wurst W, Schick JA. BMC Genomics. 2017 Nov 25;18(1):905. doi: 10.1186/s12864-017-4285-2. PubMed
Human caspase-4 detects tetra-acylated LPS and cytosolic Francisella and functions differently from murine caspase-11. Lagrange B, Benaoudia S, Wallet P, Magnotti F, Provost A, Michal F, Martin A, Di Lorenzo F, Py BF, Molinaro A, Henry T. Nat Commun. 2018 Jan 16;9(1):242. doi: 10.1038/s41467-017-02682-y. PubMed
Somatic genome editing with the RCAS-TVA-CRISPR-Cas9 system for precision tumor modeling. Oldrini B, Curiel-Garcia A, Marques C, Matia V, Uluckan O, Grana-Castro O, Torres-Ruiz R, Rodriguez-Perales S, Huse JT, Squatrito M. Nat Commun. 2018 Apr 13;9(1):1466. doi: 10.1038/s41467-018-03731-w. PubMed

Associated Plasmids

CREBBP/EP300 bromodomains are critical to sustain the GATA1/MYC regulatory axis in proliferation. Garcia-Carpizo V, Ruiz-Llorente S, Sarmentero J, Grana-Castro O, Pisano DG, Barrero MJ. Epigenetics Chromatin. 2018 Jun 8;11(1):30. doi: 10.1186/s13072-018-0197-x. PubMed
Endogenous HIF2A reporter systems for high-throughput functional screening. Zaini MN, Patel SA, Syafruddin SE, Rodrigues P, Vanharanta S. Sci Rep. 2018 Aug 13;8(1):12063. doi: 10.1038/s41598-018-30499-2. PubMed
High-Definition Analysis of Host Protein Stability during Human Cytomegalovirus Infection Reveals Antiviral Factors and Viral Evasion Mechanisms. Nightingale K, Lin KM, Ravenhill BJ, Davies C, Nobre L, Fielding CA, Ruckova E, Fletcher-Etherington A, Soday L, Nichols H, Sugrue D, Wang ECY, Moreno P, Umrania Y, Huttlin EL, Antrobus R, Davison AJ, Wilkinson GWG, Stanton RJ, Tomasec P, Weekes MP. Cell Host Microbe. 2018 Sep 12;24(3):447-460.e11. doi: 10.1016/j.chom.2018.07.011. Epub 2018 Aug 16. PubMed
AP-4 vesicles contribute to spatial control of autophagy via RUSC-dependent peripheral delivery of ATG9A. Davies AK, Itzhak DN, Edgar JR, Archuleta TL, Hirst J, Jackson LP, Robinson MS, Borner GHH. Nat Commun. 2018 Sep 27;9(1):3958. doi: 10.1038/s41467-018-06172-7. PubMed
The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1. Menzies SA, Volkmar N, van den Boomen DJ, Timms RT, Dickson AS, Nathan JA, Lehner PJ. Elife. 2018 Dec 13;7. pii: 40009. doi: 10.7554/eLife.40009. PubMed
CREBBP/EP300 Bromodomain Inhibition Affects the Proliferation of AR-Positive Breast Cancer Cell Lines. Garcia-Carpizo V, Ruiz-Llorente S, Sarmentero J, Gonzalez-Corpas A, Barrero MJ. Mol Cancer Res. 2019 Mar;17(3):720-730. doi: 10.1158/1541-7786.MCR-18-0719. Epub 2019 Jan 3. PubMed
SARM1 deficiency up-regulates XAF1, promotes neuronal apoptosis, and accelerates prion disease. Zhu C, Li B, Frontzek K, Liu Y, Aguzzi A. J Exp Med. 2019 Apr 1;216(4):743-756. doi: 10.1084/jem.20171885. Epub 2019 Mar 6. PubMed
A KLF6-driven transcriptional network links lipid homeostasis and tumour growth in renal carcinoma. Syafruddin SE, Rodrigues P, Vojtasova E, Patel SA, Zaini MN, Burge J, Warren AY, Stewart GD, Eisen T, Bihary D, Samarajiwa SA, Vanharanta S. Nat Commun. 2019 Mar 11;10(1):1152. doi: 10.1038/s41467-019-09116-x. PubMed
CRISPR-KO screen identifies Dmap1 as a regulator of chemically-induced reprogramming and differentiation of cardiac progenitors. Yu JSL, Palano G, Lim C, Moggio A, Drowley L, Plowright AT, Bohlooly-Y M, Rosen BS, Hansson EM, Wang QD, Yusa K. Stem Cells. 2019 Apr 1. doi: 10.1002/stem.3012. PubMed

Associated Plasmids

Intracellular Ca(2+) Homeostasis and Nuclear Export Mediate Exit from Naive Pluripotency. MacDougall MS, Clarke R, Merrill BJ. Cell Stem Cell. 2019 Aug 1;25(2):210-224.e6. doi: 10.1016/j.stem.2019.04.015. Epub 2019 May 16. PubMed
Targeted genetic screening in mice through haploid embryonic stem cells identifies critical genes in bone development. Bai M, Han Y, Wu Y, Liao J, Li L, Wang L, Li Q, Xing W, Chen L, Zou W, Li J. PLoS Biol. 2019 Jul 2;17(7):e3000350. doi: 10.1371/journal.pbio.3000350. eCollection 2019 Jul. PubMed
Sequential LASER ART and CRISPR Treatments Eliminate HIV-1 in a Subset of Infected Humanized Mice. Dash PK, Kaminski R, Bella R, Su H, Mathews S, Ahooyi TM, Chen C, Mancuso P, Sariyer R, Ferrante P, Donadoni M, Robinson JA, Sillman B, Lin Z, Hilaire JR, Banoub M, Elango M, Gautam N, Mosley RL, Poluektova LY, McMillan J, Bade AN, Gorantla S, Sariyer IK, Burdo TH, Young WB, Amini S, Gordon J, Jacobson JM, Edagwa B, Khalili K, Gendelman HE. Nat Commun. 2019 Jul 2;10(1):2753. doi: 10.1038/s41467-019-10366-y. PubMed
A Chemical Screen Identifies Compounds Capable of Selecting for Haploidy in Mammalian Cells. Olbrich T, Vega-Sendino M, Murga M, de Carcer G, Malumbres M, Ortega S, Ruiz S, Fernandez-Capetillo O. Cell Rep. 2019 Jul 16;28(3):597-604.e4. doi: 10.1016/j.celrep.2019.06.060. PubMed
An Evolutionarily Conserved Function of Polycomb Silences the MHC Class I Antigen Presentation Pathway and Enables Immune Evasion in Cancer. Burr ML, Sparbier CE, Chan KL, Chan YC, Kersbergen A, Lam EYN, Azidis-Yates E, Vassiliadis D, Bell CC, Gilan O, Jackson S, Tan L, Wong SQ, Hollizeck S, Michalak EM, Siddle HV, McCabe MT, Prinjha RK, Guerra GR, Solomon BJ, Sandhu S, Dawson SJ, Beavis PA, Tothill RW, Cullinane C, Lehner PJ, Sutherland KD, Dawson MA. Cancer Cell. 2019 Oct 14;36(4):385-401.e8. doi: 10.1016/j.ccell.2019.08.008. Epub 2019 Sep 26. PubMed
Pyrin dephosphorylation is sufficient to trigger inflammasome activation in familial Mediterranean fever patients. Magnotti F, Lefeuvre L, Benezech S, Malsot T, Waeckel L, Martin A, Kerever S, Chirita D, Desjonqueres M, Duquesne A, Gerfaud-Valentin M, Laurent A, Seve P, Popoff MR, Walzer T, Belot A, Jamilloux Y, Henry T. EMBO Mol Med. 2019 Oct 7:e10547. doi: 10.15252/emmm.201910547. PubMed

Associated Plasmids

Regional Gene Repression by DNA Double-Strand Breaks in G1 Phase Cells. Purman CE, Collins PL, Porter SI, Saini A, Gupta H, Sleckman BP, Oltz EM. Mol Cell Biol. 2019 Nov 25;39(24). pii: MCB.00181-19. doi: 10.1128/MCB.00181-19. Print 2019 Dec 15. PubMed
Regulation of CHD2 expression by the Chaserr long noncoding RNA gene is essential for viability. Rom A, Melamed L, Gil N, Goldrich MJ, Kadir R, Golan M, Biton I, Perry RB, Ulitsky I. Nat Commun. 2019 Nov 8;10(1):5092. doi: 10.1038/s41467-019-13075-8. PubMed
The ribosomal P-stalk couples amino acid starvation to GCN2 activation in mammalian cells. Harding HP, Ordonez A, Allen F, Parts L, Inglis AJ, Williams RL, Ron D. Elife. 2019 Nov 21;8. pii: 50149. doi: 10.7554/eLife.50149. PubMed
Tumor heterogeneity and clonal cooperation influence the immune selection of IFN-gamma-signaling mutant cancer cells. Williams JB, Li S, Higgs EF, Cabanov A, Wang X, Huang H, Gajewski TF. Nat Commun. 2020 Jan 30;11(1):602. doi: 10.1038/s41467-020-14290-4. PubMed
Reversible promoter methylation determines fluctuating expression of acute phase proteins. Zhang SC, Wang MY, Feng JR, Chang Y, Ji SR, Wu Y. Elife. 2020 Mar 30;9. pii: 51317. doi: 10.7554/eLife.51317. PubMed
Guanylate-binding proteins convert cytosolic bacteria into caspase-4 signaling platforms. Wandel MP, Kim BH, Park ES, Boyle KB, Nayak K, Lagrange B, Herod A, Henry T, Zilbauer M, Rohde J, MacMicking JD, Randow F. Nat Immunol. 2020 Aug;21(8):880-891. doi: 10.1038/s41590-020-0697-2. Epub 2020 Jun 15. PubMed
Endogenous retroviruses are a source of enhancers with oncogenic potential in acute myeloid leukaemia. Deniz O, Ahmed M, Todd CD, Rio-Machin A, Dawson MA, Branco MR. Nat Commun. 2020 Jul 14;11(1):3506. doi: 10.1038/s41467-020-17206-4. PubMed
MGMT genomic rearrangements contribute to chemotherapy resistance in gliomas. Oldrini B, Vaquero-Siguero N, Mu Q, Kroon P, Zhang Y, Galan-Ganga M, Bao Z, Wang Z, Liu H, Sa JK, Zhao J, Kim H, Rodriguez-Perales S, Nam DH, Verhaak RGW, Rabadan R, Jiang T, Wang J, Squatrito M. Nat Commun. 2020 Aug 4;11(1):3883. doi: 10.1038/s41467-020-17717-0. PubMed
Protective anti-prion antibodies in human immunoglobulin repertoires. Senatore A, Frontzek K, Emmenegger M, Chincisan A, Losa M, Reimann R, Horny G, Guo J, Fels S, Sorce S, Zhu C, George N, Ewert S, Pietzonka T, Hornemann S, Aguzzi A. EMBO Mol Med. 2020 Sep 7;12(9):e12739. doi: 10.15252/emmm.202012739. Epub 2020 Aug 10. PubMed
Regulation of neuronal commitment in mouse embryonic stem cells by the Reno1/Bahcc1 locus. Hezroni H, Ben-Tov Perry R, Gil N, Degani N, Ulitsky I. EMBO Rep. 2020 Nov 5;21(11):e51264. doi: 10.15252/embr.202051264. Epub 2020 Sep 24. PubMed
Quantitative Proteomics Analysis of Lytic KSHV Infection in Human Endothelial Cells Reveals Targets of Viral Immune Modulation. Gabaev I, Williamson JC, Crozier TWM, Schulz TF, Lehner PJ. Cell Rep. 2020 Oct 13;33(2):108249. doi: 10.1016/j.celrep.2020.108249. PubMed
Targeting the ubiquitin-proteasome system in a pancreatic cancer subtype with hyperactive MYC. Lankes K, Hassan Z, Doffo MJ, Schneeweis C, Lier S, Ollinger R, Rad R, Kramer OH, Keller U, Saur D, Reichert M, Schneider G, Wirth M. Mol Oncol. 2020 Dec;14(12):3048-3064. doi: 10.1002/1878-0261.12835. Epub 2020 Nov 8. PubMed
De Novo VPS4A Mutations Cause Multisystem Disease with Abnormal Neurodevelopment. Rodger C, Flex E, Allison RJ, Sanchis-Juan A, Hasenahuer MA, Cecchetti S, French CE, Edgar JR, Carpentieri G, Ciolfi A, Pantaleoni F, Bruselles A, Onesimo R, Zampino G, Marcon F, Siniscalchi E, Lees M, Krishnakumar D, McCann E, Yosifova D, Jarvis J, Kruer MC, Marks W, Campbell J, Allen LE, Gustincich S, Raymond FL, Tartaglia M, Reid E. Am J Hum Genet. 2020 Dec 3;107(6):1129-1148. doi: 10.1016/j.ajhg.2020.10.012. Epub 2020 Nov 12. PubMed
SLE non-coding genetic risk variant determines the epigenetic dysfunction of an immune cell specific enhancer that controls disease-critical microRNA expression. Hou G, Harley ITW, Lu X, Zhou T, Xu N, Yao C, Qin Y, Ouyang Y, Ma J, Zhu X, Yu X, Xu H, Dai D, Ding H, Yin Z, Ye Z, Deng J, Zhou M, Tang Y, Namjou B, Guo Y, Weirauch MT, Kottyan LC, Harley JB, Shen N. Nat Commun. 2021 Jan 8;12(1):135. doi: 10.1038/s41467-020-20460-1. PubMed
No evidence for basigin/CD147 as a direct SARS-CoV-2 spike binding receptor. Shilts J, Crozier TWM, Greenwood EJD, Lehner PJ, Wright GJ. Sci Rep. 2021 Jan 11;11(1):413. doi: 10.1038/s41598-020-80464-1. PubMed
Cut-like homeobox 1 (CUX1) tumor suppressor gene haploinsufficiency induces apoptosis evasion to sustain myeloid leukemia. Supper E, Rudat S, Iyer V, Droop A, Wong K, Spinella JF, Thomas P, Sauvageau G, Adams DJ, Wong CC. Nat Commun. 2021 Apr 30;12(1):2482. doi: 10.1038/s41467-021-22750-8. PubMed
Functional annotation of the 2q35 breast cancer risk locus implicates a structural variant in influencing activity of a long-range enhancer element. Baxter JS, Johnson N, Tomczyk K, Gillespie A, Maguire S, Brough R, Fachal L, Michailidou K, Bolla MK, Wang Q, Dennis J, Ahearn TU, Andrulis IL, Anton-Culver H, Antonenkova NN, Arndt V, Aronson KJ, Augustinsson A, Becher H, Beckmann MW, Behrens S, Benitez J, Bermisheva M, Bogdanova NV, Bojesen SE, Brenner H, Brucker SY, Cai Q, Campa D, Canzian F, Castelao JE, Chan TL, Chang-Claude J, Chanock SJ, Chenevix-Trench G, Choi JY, Clarke CL, Colonna S, Conroy DM, Couch FJ, Cox A, Cross SS, Czene K, Daly MB, Devilee P, Dork T, Dossus L, Dwek M, Eccles DM, Ekici AB, Eliassen AH, Engel C, Fasching PA, Figueroa J, Flyger H, Gago-Dominguez M, Gao C, Garcia-Closas M, Garcia-Saenz JA, Ghoussaini M, Giles GG, Goldberg MS, Gonzalez-Neira A, Guenel P, Gundert M, Haeberle L, Hahnen E, Haiman CA, Hall P, Hamann U, Hartman M, Hatse S, Hauke J, Hollestelle A, Hoppe R, Hopper JL, Hou MF, Ito H, Iwasaki M, Jager A, Jakubowska A, Janni W, John EM, Joseph V, Jung A, Kaaks R, Kang D, Keeman R, Khusnutdinova E, Kim SW, Kosma VM, Kraft P, Kristensen VN, Kubelka-Sabit K, Kurian AW, Kwong A, Lacey JV, Lambrechts D, Larson NL, Larsson SC, Le Marchand L, Lejbkowicz F, Li J, Long J, Lophatananon A, Lubinski J, Mannermaa A, Manoochehri M, Manoukian S, Margolin S, Matsuo K, Mavroudis D, Mayes R, Menon U, Milne RL, Mohd Taib NA, Muir K, Muranen TA, Murphy RA, Nevanlinna H, O'Brien KM, Offit K, Olson JE, Olsson H, Park SK, Park-Simon TW, Patel AV, Peterlongo P, Peto J, Plaseska-Karanfilska D, Presneau N, Pylkas K, Rack B, Rennert G, Romero A, Ruebner M, Rudiger T, Saloustros E, Sandler DP, Sawyer EJ, Schmidt MK, Schmutzler RK, Schneeweiss A, Schoemaker MJ, Shah M, Shen CY, Shu XO, Simard J, Southey MC, Stone J, Surowy H, Swerdlow AJ, Tamimi RM, Tapper WJ, Taylor JA, Teo SH, Teras LR, Terry MB, Toland AE, Tomlinson I, Truong T, Tseng CC, Untch M, Vachon CM, van den Ouweland AMW, Wang SS, Weinberg CR, Wendt C, Winham SJ, Winqvist R, Wolk A, Wu AH, Yamaji T, Zheng W, Ziogas A, Pharoah PDP, Dunning AM, Easton DF, Pettitt SJ, Lord CJ, Haider S, Orr N, Fletcher O. Am J Hum Genet. 2021 Jul 1;108(7):1190-1203. doi: 10.1016/j.ajhg.2021.05.013. Epub 2021 Jun 18. PubMed

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