pCFD5 Citations (63)
Originally described in: Augmenting CRISPR applications in Drosophila with tRNA-flanked sgRNAs.Port F, Bullock SL Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 2016 Sep 5. PubMed Journal
Articles Citing pCFD5
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| Tissue-specific (ts)CRISPR as an efficient strategy for in vivo screening in Drosophila. Meltzer H, Marom E, Alyagor I, Mayseless O, Berkun V, Segal-Gilboa N, Unger T, Luginbuhl D, Schuldiner O. Nat Commun. 2019 May 8;10(1):2113. doi: 10.1038/s41467-019-10140-0. PubMed |
| CRISPR-induced double-strand breaks trigger recombination between homologous chromosome arms. Brunner E, Yagi R, Debrunner M, Beck-Schneider D, Burger A, Escher E, Mosimann C, Hausmann G, Basler K. Life Sci Alliance. 2019 Jun 13;2(3). pii: 2/3/e201800267. doi: 10.26508/lsa.201800267. Print 2019 Jun. PubMed |
| Thermosensitive alternative splicing senses and mediates temperature adaptation in Drosophila. Martin Anduaga A, Evantal N, Patop IL, Bartok O, Weiss R, Kadener S. Elife. 2019 Nov 8;8. pii: 44642. doi: 10.7554/eLife.44642. PubMed |
| Glycogen branching enzyme controls cellular iron homeostasis via Iron Regulatory Protein 1 and mitoNEET. Huynh N, Ou Q, Cox P, Lill R, King-Jones K. Nat Commun. 2019 Nov 29;10(1):5463. doi: 10.1038/s41467-019-13237-8. PubMed |
| Spatial and temporal control of gene manipulation in Drosophila via drug-activated Cas9 nucleases. Huynh N, Wang S, King-Jones K. Insect Biochem Mol Biol. 2020 May;120:103336. doi: 10.1016/j.ibmb.2020.103336. Epub 2020 Feb 24. PubMed |
| A fly model establishes distinct mechanisms for synthetic CRISPR/Cas9 sex distorters. Fasulo B, Meccariello A, Morgan M, Borufka C, Papathanos PA, Windbichler N. PLoS Genet. 2020 Mar 13;16(3):e1008647. doi: 10.1371/journal.pgen.1008647. eCollection 2020 Mar. PubMed |
| FGF Pyramus Has a Transmembrane Domain and Cell-Autonomous Function in Polarity. Stepanik V, Sun J, Stathopoulos A. Curr Biol. 2020 Aug 17;30(16):3141-3153.e5. doi: 10.1016/j.cub.2020.06.006. Epub 2020 Jul 2. PubMed |
SPARC enables genetic manipulation of precise proportions of cells.
Isaacman-Beck J, Paik KC, Wienecke CFR, Yang HH, Fisher YE, Wang IE, Ishida IG, Maimon G, Wilson RI, Clandinin TR.
Nat Neurosci. 2020 Sep;23(9):1168-1175. doi: 10.1038/s41593-020-0668-9. Epub 2020 Jul 20.
PubMed
Associated Plasmids |
| Analysis of Drosophila Atg8 proteins reveals multiple lipidation-independent roles. Jipa A, Vedelek V, Merenyi Z, Urmosi A, Takats S, Kovacs AL, Horvath GV, Sinka R, Juhasz G. Autophagy. 2021 Sep;17(9):2565-2575. doi: 10.1080/15548627.2020.1856494. Epub 2020 Dec 17. PubMed |
Precise genome engineering in Drosophila using prime editing.
Bosch JA, Birchak G, Perrimon N.
Proc Natl Acad Sci U S A. 2021 Jan 5;118(1). pii: 2021996118. doi: 10.1073/pnas.2021996118.
PubMed
Associated Plasmids |
| Ecd promotes U5 snRNP maturation and Prp8 stability. Erkelenz S, Stankovic D, Mundorf J, Bresser T, Claudius AK, Boehm V, Gehring NH, Uhlirova M. Nucleic Acids Res. 2021 Feb 22;49(3):1688-1707. doi: 10.1093/nar/gkaa1274. PubMed |
| Genome editing efficiency of four Drosophila suzukii endogenous U6 promoters. Ni XY, Lu WJ, Qiao X, Huang J. Insect Mol Biol. 2021 Aug;30(4):420-426. doi: 10.1111/imb.12707. Epub 2021 May 24. PubMed |
| A developmental framework linking neurogenesis and circuit formation in the Drosophila CNS. Mark B, Lai SL, Zarin AA, Manning L, Pollington HQ, Litwin-Kumar A, Cardona A, Truman JW, Doe CQ. Elife. 2021 May 11;10. pii: 67510. doi: 10.7554/eLife.67510. PubMed |
Single-cell lineage tracing of metastatic cancer reveals selection of hybrid EMT states.
Simeonov KP, Byrns CN, Clark ML, Norgard RJ, Martin B, Stanger BZ, Shendure J, McKenna A, Lengner CJ.
Cancer Cell. 2021 Aug 9;39(8):1150-1162.e9. doi: 10.1016/j.ccell.2021.05.005. Epub 2021 Jun 10.
PubMed
Associated Plasmids |
| Proteolytic activation of Growth-blocking peptides triggers calcium responses through the GPCR Mthl10 during epithelial wound detection. O'Connor JT, Stevens AC, Shannon EK, Akbar FB, LaFever KS, Narayanan NP, Gailey CD, Hutson MS, Page-McCaw A. Dev Cell. 2021 Aug 9;56(15):2160-2175.e5. doi: 10.1016/j.devcel.2021.06.020. Epub 2021 Jul 21. PubMed |
| A cis-regulatory element promoting increased transcription at low temperature in cultured ectothermic Drosophila cells. Bai Y, Caussinus E, Leo S, Bosshardt F, Myachina F, Rot G, Robinson MD, Lehner CF. BMC Genomics. 2021 Oct 28;22(1):771. doi: 10.1186/s12864-021-08057-4. PubMed |
| The decoy SNARE Tomosyn sets tonic versus phasic release properties and is required for homeostatic synaptic plasticity. Sauvola CW, Akbergenova Y, Cunningham KL, Aponte-Santiago NA, Littleton JT. Elife. 2021 Oct 29;10. pii: 72841. doi: 10.7554/eLife.72841. PubMed |
| The role of the epidermis enhancer element in positive and negative transcriptional regulation of ebony in Drosophila melanogaster. Akiyama N, Sato S, Tanaka KM, Sakai T, Takahashi A. G3 (Bethesda). 2022 Mar 4;12(3). pii: 6506522. doi: 10.1093/g3journal/jkac010. PubMed |
| Loss of ubiquitinated protein autophagy is compensated by persistent cnc/NFE2L2/Nrf2 antioxidant responses. Bhattacharjee A, Urmosi A, Jipa A, Kovacs L, Deak P, Szabo A, Juhasz G. Autophagy. 2022 Feb 20:1-12. doi: 10.1080/15548627.2022.2037852. PubMed |
| Ir56b is an atypical ionotropic receptor that underlies appetitive salt response in Drosophila. Dweck HKM, Talross GJS, Luo Y, Ebrahim SAM, Carlson JR. Curr Biol. 2022 Apr 25;32(8):1776-1787.e4. doi: 10.1016/j.cub.2022.02.063. Epub 2022 Mar 15. PubMed |
| Dual nicotinic acetylcholine receptor subunit gene knockouts reveal limits to functional redundancy. Chen W, Gu X, Yang YT, Batterham P, Perry T. Pestic Biochem Physiol. 2022 Jun;184:105118. doi: 10.1016/j.pestbp.2022.105118. Epub 2022 May 11. PubMed |
| Damage-responsive neuro-glial clusters coordinate the recruitment of dormant neural stem cells in Drosophila. Simoes AR, Neto M, Alves CS, Santos MB, Fernandez-Hernandez I, Veiga-Fernandes H, Brea D, Dura I, Encinas JM, Rhiner C. Dev Cell. 2022 Jul 11;57(13):1661-1675.e7. doi: 10.1016/j.devcel.2022.05.015. Epub 2022 Jun 17. PubMed |
| Kinetic principles underlying pioneer function of GAGA transcription factor in live cells. Tang X, Li T, Liu S, Wisniewski J, Zheng Q, Rong Y, Lavis LD, Wu C. Nat Struct Mol Biol. 2022 Jul;29(7):665-676. doi: 10.1038/s41594-022-00800-z. Epub 2022 Jul 14. PubMed |
| Copy number changes in co-expressed odorant receptor genes enable selection for sensory differences in drosophilid species. Auer TO, Alvarez-Ocana R, Cruchet S, Benton R, Arguello JR. Nat Ecol Evol. 2022 Sep;6(9):1343-1353. doi: 10.1038/s41559-022-01830-y. Epub 2022 Jul 21. PubMed |
| A Notch-dependent transcriptional mechanism controls expression of temporal patterning factors in Drosophila medulla. Ray A, Li X. Elife. 2022 Aug 30;11:e75879. doi: 10.7554/eLife.75879. PubMed |
| Pheromone sensing in Drosophila requires support cell-expressed Osiris 8. Scalzotto M, Ng R, Cruchet S, Saina M, Armida J, Su CY, Benton R. BMC Biol. 2022 Oct 11;20(1):230. doi: 10.1186/s12915-022-01425-w. PubMed |
| Shadow enhancers modulate distinct transcriptional parameters that differentially effect downstream patterning events. Whitney PH, Shrestha B, Xiong J, Zhang T, Rushlow CA. Development. 2022 Nov 1;149(21):dev200940. doi: 10.1242/dev.200940. Epub 2022 Nov 1. PubMed |
| A critical developmental window for ELAV/Hu-dependent mRNA signatures at the onset of neuronal differentiation. Carrasco J, Mateos F, Hilgers V. Cell Rep. 2022 Oct 25;41(4):111542. doi: 10.1016/j.celrep.2022.111542. PubMed |
| Coordinated efforts of different actin filament populations are needed for optimal cell wound repair. Hui J, Nakamura M, Dubrulle J, Parkhurst SM. Mol Biol Cell. 2023 Mar 1;34(3):ar15. doi: 10.1091/mbc.E22-05-0155. Epub 2023 Jan 4. PubMed |
| Systematic analysis of RhoGEF/GAP localizations uncovers regulators of mechanosensing and junction formation during epithelial cell division. di Pietro F, Osswald M, De Las Heras JM, Cristo I, Lopez-Gay J, Wang Z, Pelletier S, Gaugue I, Leroy A, Martin C, Morais-de-Sa E, Bellaiche Y. Curr Biol. 2023 Mar 13;33(5):858-874.e7. doi: 10.1016/j.cub.2023.01.028. Epub 2023 Feb 13. PubMed |
| Drosophila oocyte specification is maintained by the dynamic duo of microtubule polymerase Mini spindles/XMAP215 and dynein. Lu W, Lakonishok M, Gelfand VI. bioRxiv. 2023 Mar 10:2023.03.09.531953. doi: 10.1101/2023.03.09.531953. Preprint. PubMed |
| Heterodimerization-dependent secretion of bone morphogenetic proteins in Drosophila. Bauer M, Aguilar G, Wharton KA, Matsuda S, Affolter M. Dev Cell. 2023 Apr 24;58(8):645-659.e4. doi: 10.1016/j.devcel.2023.03.008. Epub 2023 Apr 12. PubMed |
| Stochastic RNA editing of the Complexin C-terminus within single neurons regulates neurotransmitter release. Brija EA, Guan Z, Jetti SK, Littleton JT. bioRxiv. 2023 May 30:2023.05.30.542887. doi: 10.1101/2023.05.30.542887. Preprint. PubMed |
| Two-step CRISPR-Cas9 protocol for transposable element deletion in D. melanogaster natural populations. Merenciano M, Aguilera L, Gonzalez J. STAR Protoc. 2023 Sep 15;4(3):102501. doi: 10.1016/j.xpro.2023.102501. Epub 2023 Aug 15. PubMed |
| The RNF220 domain nuclear factor Teyrha-Meyrha (Tey) regulates the migration and differentiation of specific visceral and somatic muscles in Drosophila. Frasch M, Ismat A, Reim I, Raufer J. Development. 2023 Sep 15;150(18):dev201457. doi: 10.1242/dev.201457. Epub 2023 Sep 14. PubMed |
| Stochastic RNA editing of the Complexin C-terminus within single neurons regulates neurotransmitter release. Brija EA, Guan Z, Jetti SK, Littleton JT. Cell Rep. 2023 Sep 26;42(9):113152. doi: 10.1016/j.celrep.2023.113152. Epub 2023 Sep 19. PubMed |
| The dynamic duo of microtubule polymerase Mini spindles/XMAP215 and cytoplasmic dynein is essential for maintaining Drosophila oocyte fate. Lu W, Lakonishok M, Gelfand VI. Proc Natl Acad Sci U S A. 2023 Sep 26;120(39):e2303376120. doi: 10.1073/pnas.2303376120. Epub 2023 Sep 18. PubMed |
| Sensory neuron population expansion enhances odor tracking without sensitizing projection neurons. Takagi S, Sancer G, Abuin L, Stupski SD, Arguello JR, Prieto-Godino LL, Stern DL, Cruchet S, Alvarez-Ocana R, Wienecke CFR, van Breugel F, Jeanne JM, Auer TO, Benton R. bioRxiv [Preprint]. 2024 Feb 28:2023.09.15.556782. doi: 10.1101/2023.09.15.556782. PubMed |
| Impact of Drosophila LIM homeodomain protein Apterous on the morphology of the adult mushroom body. Nakano H, Sakai T. Biochem Biophys Res Commun. 2023 Nov 19;682:77-84. doi: 10.1016/j.bbrc.2023.09.071. Epub 2023 Sep 25. PubMed |
| Metabolic regulation of proteome stability via N-terminal acetylation controls male germline stem cell differentiation and reproduction. Francois CM, Pihl T, Dunoyer de Segonzac M, Herault C, Hudry B. Nat Commun. 2023 Oct 23;14(1):6737. doi: 10.1038/s41467-023-42496-9. PubMed |
| Molecular and functional characterization of the Drosophila melanogaster conserved smORFome. Bosch JA, Keith N, Escobedo F, Fisher WW, LaGraff JT, Rabasco J, Wan KH, Weiszmann R, Hu Y, Kondo S, Brown JB, Perrimon N, Celniker SE. Cell Rep. 2023 Nov 28;42(11):113311. doi: 10.1016/j.celrep.2023.113311. Epub 2023 Oct 26. PubMed |
| Germline Editing of Drosophila Using CRISPR-Cas9-Based Cytosine and Adenine Base Editors. Thakkar N, Hejzlarova A, Brabec V, Dolezel D. CRISPR J. 2023 Dec;6(6):557-569. doi: 10.1089/crispr.2023.0026. Epub 2023 Nov 2. PubMed |
| Regulatory network in heat stress response in parasitoid wasp focusing on Xap5 heat stress regulator. Xiong S, Yu K, Lin H, Ye X, Xiao S, Yang Y, Stanley DW, Song Q, Fang Q, Ye G. iScience. 2023 Dec 3;27(1):108622. doi: 10.1016/j.isci.2023.108622. eCollection 2024 Jan 19. PubMed |
| Loss of the endoplasmic reticulum protein Tmem208 affects cell polarity, development, and viability. Dutta D, Kanca O, Shridharan RV, Marcogliese PC, Steger B, Morimoto M, Frost FG, Macnamara E, Wangler MF, Yamamoto S, Jenny A, Adams D, Malicdan MC, Bellen HJ. Proc Natl Acad Sci U S A. 2024 Feb 27;121(9):e2322582121. doi: 10.1073/pnas.2322582121. Epub 2024 Feb 21. PubMed |
| Drosophila Evi5 is a critical regulator of intracellular iron transport via transferrin and ferritin interactions. Soltani S, Webb SM, Kroll T, King-Jones K. Nat Commun. 2024 May 14;15(1):4045. doi: 10.1038/s41467-024-48165-9. PubMed |
| Targeted mutagenesis of specific genomic DNA sequences in animals for the in vivo generation of variant libraries. Falo-Sanjuan J, Diaz-Tirado Y, Turner MA, Rourke O, Davis J, Medrano C, Haines J, McKenna J, Karshenas A, Eisen MB, Garcia HG. bioRxiv [Preprint]. 2024 Dec 21:2024.06.10.598328. doi: 10.1101/2024.06.10.598328. PubMed |
| AdamTS proteases control basement membrane heterogeneity and organ shape in Drosophila. Topfer U, Ryu J, Guerra Santillan KY, Schulze J, Fischer-Friedrich E, Tanentzapf G, Dahmann C. Cell Rep. 2024 Jul 23;43(7):114399. doi: 10.1016/j.celrep.2024.114399. Epub 2024 Jun 28. PubMed |
| Protocol for generating in-frame seamless knockins in Drosophila using the SEED/Harvest technology. Aguilar G, Bauer M, Vigano MA, Guerrero I, Affolter M. STAR Protoc. 2024 Sep 20;5(3):102932. doi: 10.1016/j.xpro.2024.102932. Epub 2024 Jul 11. PubMed |
| Cellular sex throughout the organism underlies somatic sexual differentiation. Herault C, Pihl T, Hudry B. Nat Commun. 2024 Aug 13;15(1):6925. doi: 10.1038/s41467-024-51228-6. PubMed |
| Olfactory sensory neuron population expansions influence projection neuron adaptation and enhance odour tracking. Takagi S, Sancer G, Abuin L, Stupski SD, Roman Arguello J, Prieto-Godino LL, Stern DL, Cruchet S, Alvarez-Ocana R, Wienecke CFR, van Breugel F, Jeanne JM, Auer TO, Benton R. Nat Commun. 2024 Aug 15;15(1):7041. doi: 10.1038/s41467-024-50808-w. PubMed |
| The Drosophila maternal-effect gene abnormal oocyte (ao) does not repress histone gene expression. Takenaka R, Simmerman SM, Schmidt CA, Albanese EH, Rieder LE, Malik HS. bioRxiv [Preprint]. 2024 Sep 18:2024.09.17.613536. doi: 10.1101/2024.09.17.613536. PubMed |
| The WIRS motifs in Fat2 are required for Drosophila egg chamber rotation but not for elongation. Bhatt A, Ruffine V, Topfer U, Ryu J, Fischer-Friedrich E, Dahmann C. Development. 2025 Jan 15;152(2):DEV204201. doi: 10.1242/dev.204201. Epub 2025 Jan 17. PubMed |
| Interactions of N- and C-terminal parts of Ana1 permitting centriole duplication but not elongation. Nagy A, Kovacs L, Rangone H, Fu J, Ladinsky M, Glover DM. Open Biol. 2025 Feb;15(2):240325. doi: 10.1098/rsob.240325. Epub 2025 Feb 5. PubMed |
| Active zone maturation state controls synaptic output and release mode and is differentially regulated by neuronal activity. Akbergenova Y, Matthias J, Littleton JT. bioRxiv [Preprint]. 2025 Feb 3:2025.02.03.636302. doi: 10.1101/2025.02.03.636302. PubMed |
| Formin 3 stabilizes the cytoskeleton of Drosophila tendon cells, thus enabling them to resist muscle tensile forces. Pissarek H, Huang N, Frasch LH, Aberle H, Frasch M. J Cell Sci. 2025 Apr 1;138(7):jcs263543. doi: 10.1242/jcs.263543. Epub 2025 Apr 11. PubMed |
| The proximal enhancer of the snail gene mediates negative autoregulatory feedback in Drosophila melanogaster. Dunipace L, McGehee JM, Irizarry J, Stathopoulos A. Genetics. 2025 Jun 4;230(2):iyaf058. doi: 10.1093/genetics/iyaf058. PubMed |
| The transcription factor Traffic jam orchestrates the somatic piRNA pathway in Drosophila ovaries. Alizada A, Martins A, Mouniee N, Rodriguez Suarez JV, Bertin B, Gueguen N, Mirouse V, Papameletiou AM, Rivera AJ, Lau NC, Akkouche A, Maupetit-Mehouas S, Hannon GJ, Czech Nicholson B, Brasset E. Cell Rep. 2025 Apr 22;44(4):115453. doi: 10.1016/j.celrep.2025.115453. Epub 2025 Apr 9. PubMed |
| Olfactory projection neuron rewiring in the brain of an ecological specialist. Durr BR, Bertolini E, Takagi S, Pascual J, Abuin L, Lucarelli G, Benton R, Auer TO. Cell Rep. 2025 May 27;44(5):115615. doi: 10.1016/j.celrep.2025.115615. Epub 2025 Apr 25. PubMed |
| New molecular components of high and low affinity iron import systems in Drosophila. Soltani S, Yan M, Yu Q, Abd Elhafiz A, Pfriem E, Webb SM, Kroll T, Bahena Lopez JM, Missirlis F, King-Jones K. Nat Commun. 2025 Jul 1;16(1):5662. doi: 10.1038/s41467-025-60758-6. PubMed |
| GSK-3beta coordinates axonal microtubule organisation through Shot and Tau. Voelzmann A, Nuhu-Soso L, Roof A, Patel S, Bennett H, Adamson A, Bentley M, Evans GJO, Hahn I. bioRxiv [Preprint]. 2025 May 1:2024.09.08.611864. doi: 10.1101/2024.09.08.611864. PubMed |
| The CPEB ortholog Orb2 regulates brain size through the TRIM-NHL RNA-binding protein, Brain tumor. Hailstock T, Buehler J, Robinson BV, Low TCH, Hua Y, Adebambo TH, Govaerts J, Schoborg TA, Lipshitz HD, Lerit DA. bioRxiv [Preprint]. 2025 Jul 22:2025.07.18.665534. doi: 10.1101/2025.07.18.665534. PubMed |
| Biallelic variants in ARHGAP19 cause a progressive inherited motor-predominant neuropathy. Dominik N, Efthymiou S, Record CJ, Miao X, Lin RQ, Parmar JM, Scardamaglia A, Maroofian R, Lowe SA, Aughey GN, Wilson AD, Curro R, Schnekenberg RP, Alavi S, Leclaire L, He Y, Zhelcheska K, Bellaiche Y, Gaugue I, Skorupinska M, Van de Vondel L, Da'as SI, Turchetti V, Gungor S, Monahan GV, Ghayoor Karimiani E, Jamshidi Y, Lamont PJ, Armirola-Ricaurte C, Topaloglu H, Jordanova A, Zaman M, Banu SH, Marques W, Tomaselli PJ, Aynekin B, Cansu A, Per H, Gulec A, Alvi JR, Sultan T, Khan A, Zifarelli G, Ibrahim S, Mancini GMS, Motazacker MM, Brusse E, Lupo V, Sevilla T, Basak AN, Tekgul S, Palvadeau RJ, Baets J, Parman Y, Cakar A, Horvath R, Haack TB, Stahl JH, Grundmann-Hauser K, Park J, Zuchner S, Laing NG, Wilson LA, Rossor AM, Polke J, Figueiredo FB, Pessoa A, Kok F, Coimbra-Neto AR, Franca MC Jr, Ravenscroft G, Hamed SA, Chung WK, Pittman AM, Osborn DP, Hanna M, Cortese A, Reilly MM, Jepson JE, Lamarche-Vane N, Houlden H. J Clin Invest. 2025 Oct 14;135(23):e184474. doi: 10.1172/JCI184474. eCollection 2025 Dec 1. PubMed |
| Vnd and En are expressed in orthogonal stripes and act in a brief competence window to combinatorially specify NB7-1 and its early lineage. Anderson NLQ, Lai SL, Doe CQ. Dev Biol. 2026 Apr;532:73-82. doi: 10.1016/j.ydbio.2026.01.008. Epub 2026 Jan 14. PubMed |
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