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AAV Packaged on Request
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Addgene
Addgene has been an exceptionally useful resource for us, both because they can be trusted to supply our plasmids to other labs efficiently, and because we ourselves are constantly ordering reagents of interest that other labs have deposited. Keep up the good work!
La Jolla Institute for Immunology (La Jolla, CA)
Our optogenetic tools are sweeping throughout neuroscience, helping scientists figure out how the brain works. Without Addgene helping provide these to the world, neuroscience would be moving at a much slower pace.
Massachusetts Institute of Technology (Cambridge, MA)
Tools of the Day
Plasmid
Depositor: Bas Teusink
Purpose: equimolar expression of ymStayGold and mCherry in budding yeast
Viral Prep
Depositor: Jonathan Ting
Purpose: Ready-to-use AAV PHP.eB particles produced from AiP14361: pAAV-AiE0452h_3xC2-minBG-jGCaMP8s-WPRE3-BGHpA (Alias: CN4361) (#214857). minBG-driven expression of jGCaMP8s in striatal indirect pathway medium spiny neurons (D2 MSNs). These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.
Antibody
Depositor: Melina Fan
Purpose: Anti-DYKDDDDK recombinant mouse monoclonal antibody; binds to FLAG tag sequence.