Pooled Libraries
Addgene depositors have shared 388 pooled libraries — tens to millions of plasmids in a single tube all built with the same backbone and only differing in a small region. Find pooled libraries for barcoding, CRISPR, screening, and surface display experiments below.
Barcoding Libraries
Barcode libraries contain large numbers of plasmids, each with a short randomized unique nucleotide sequence, or barcode used to mark individual cells. These barcodes will then be inherited by any daughter cells, making barcode libraries ideal for lineage tracing. These libraries are also commonly used to monitor the dynamics of heterogeneous tumors.
| ID | Library | Description | Vector Type | # of Barcodes | Fluorescent Reporter | Selectable Marker | PI | |
|---|---|---|---|---|---|---|---|---|
| 180483 186334 186335 |
STICR Library | Used for labeling a starting cell population and for clonal lineage/fate tracing. | Lentiviral | ~50–70 million | EGFP | N/A | Tomasz Nowakowski | |
| 155257 | Watermelon Library | Used for simultaneous tracing of clonal lineages as well as the transcriptional and proliferative state of each cell in the population. | Lentiviral | 5×106 | mCherry, mNeonGreen | N/A | Aviv Regev, Joan Brugge | |
| 194097 194098 217420 217421 217422 217423 217424 1000000239 |
Single-cell quantitative expression reporters (scQers) Libraries | Used for integrating your regulatory elements of interest for single-cell expression reporter assays. | Mammalian Expression | ~1,200,000 ~1,200,000 250 250 250 250 250 1,250 |
N/A EGFP EGFP EGFP EGFP EGFP EGFP EGFP |
N/A N/A Puromycin Puromycin Puromycin Puromycin Puromycin Puromycin |
Jay Shendure | |
| 179774 179775 179776 |
SPLINTR Pooled Libraries | Used for tracing clonal lineages using bulk and single-cell readouts. | Lentiviral | 170,000 1.3 million 670,000 |
EGFP mCherry EBFP2 |
N/A | Mark Dawson | |
| 140024 | LARRY Barcode Library Version 1 (Discontinued) | Used for reconstructing transcriptional trajectories and clonal and state-fate analyses in differentiating cell populations. | Lentiviral | 245,979 | EGFP | N/A | Fernando Camargo | |
| 233213 233214 233215 233216 233697 |
LARRY Barcode Library Version 2 | Used for reconstructing transcriptional trajectories and clonal and state-fate analyses in differentiating cell populations. | Lentiviral | >50 million | T-Sapphire T-Sapphire EGFP EGFP mScarlet |
N/A | Alejo Rodriguez-Fraticelli | |
| 178943 | macsGESTALT Library | PiggyBac barcoding library for inducible lineage recording system. | Mammalian Expression | theoretically >1 million | N/A | Puromycin | Christopher Lengner | |
| 179851 179852 |
Moffat Lab - Barcode Libraries | Library with Puromycin and ZsGreen markers. | Lentiviral | >106 | ZsGreen | Puromycin | Jason Moffat | |
| 67267 69830 |
ClonTracer Library | Used to conduct positive selection screens for cancer cells resistant to a given treatment. Learn how the library was used to identify pools of cells that were resistant pre-treatment. | Lentiviral | ~73 million | TagRFP | Puromycin | Frank Stegmeier | |
| 115643 115644 115645 206045 |
CellTag Barcode Library | Used to combinatorially index cells for single-cell analysis of clonal dynamics. Available as a viral prep. | Lentiviral | 19,973 4,934 5,737 ~80,000 |
EGFP | N/A | Samantha Morris | |
| 85968 | Perturb-seq Guide Barcodes (GBC) Library | Used for cloning gRNA sublibraries for single cell CRISPR analysis. | Lentiviral | >100,000 | TagBFP | Puromycin | Jonathan Weissman | |
| 170392 | Target Site Library (PCT48) | Library backbone contains three Cas9-targetable spacer sequences with a random 14-bp barcode. | Lentiviral | theoretically >100 million | sfGFP | Puromycin | Jonathan Weissman | |
| 229136 | EGFP miSFIT Lineage Barcoding Library | Create a lentiviral barcoding pool that is robustly expressed in human PSC-derived hematopoietic cells. | Lentiviral | >500,000 | EGFP | N/A | Peter Zandstra | |
| 238546 238547 238548 |
PEtracer Lentiviral Libraries | Libraries containing selectable markers that encode ground truth static marks for phylogenetic reconstructions. | Lentiviral | ~100,000–150,000 ~100,000–150,000 2,170 |
N/A N/A mCherry |
Puromycin Blasticidin N/A |
Jonathan Weissman | |
| 227193 | pMuSIC pool v1.0 | Fluorescent barcoding library with 153 unique fluorescent protein barcodes made from 2-way combinations of 18 individual fluorescent proteins. | Mammalian Expression | 153 | Multiple | N/A | Marc Birtwistle | |
| 211610 | PolyA Barcode Library | Retroviral barcoding library for capturing the transcriptome and cell identities. | Retroviral | >3 million | EGFP | N/A | Yuejun Chen | |
| 232068 | CRASP-Seq CBC Backbone Library | Used for cloning custom libraries tailored to CRASP-Seq modulation approaches. A 12-nucleotide cell barcode enables library diversification for downstream cloning. | Lentiviral | >107 | N/A | Puromycin | Thomas Gonatopoulos-Pournatzis | |
| 216133 216134 |
Human Genome-wide Intron Tagging Libraries | Intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach. | Lentiviral | 90,657 sgRNAs, 1,000 control 72,580 sgRNAs, 1,000 control |
N/A | Puromycin Blasticidin |
Stefan Kubicek | |
| 227671 227672 | Rabies Barcode Pooled Libraries | G-deleted rabies virus barcoding libraries for neuronal tracing and high-fidelity connectivity mapping. | G-deleted Rabies Virus | ~125,000,000 | dTomato oScarlet |
N/A | Tomasz Nowakowski, Cathryn Cadwell |
CRISPR Pooled Libraries
CRISPR is a useful tool for genetic screening experiments, due to the relative ease of designing gRNAs and the ability to modify virtually any genetic locus. CRISPR pooled libraries deliver many gRNAs at once, targeting many genes in one experiment. These libraries may be designed to target all the genes in the genome of an organism or a subset of genes, such as those involved in a certain pathway or biological process. In a CRISPR screening experiment, target cells are treated with the pooled library to create a population of mutant cells that are then screened for a phenotype of interest. Screening experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic locus. You can learn more about CRISPR-based gene regulation on our CRISPR Guide.
There are multiple types of CRISPR libraries:
- Knockout: CRISPR knockout libraries are designed to create insertions or deletions in targeted genes across the genome, rendering them nonfunctional.
- Activation: CRISPR activation libraries use gRNAs to guide dCas9 bound to a transcriptional activator to target genes and thereby activate their expression.
- Inhibition: CRISPR inhibition libraries use gRNAs to guide dCas9 bound to a transcriptional repressor to target genes, block transcription initiation, and thereby repress, or knockdown, their expression.
- Base Edit: CRISPR base edit libraries use gRNAs to guide base editors to convert one base to another.
- Prime Edit: CRISPR prime edit libraries use prime editing guide RNAs (pegRNAs) to target genes for creating insertions, deletions, and base edits.
- Other: CRISPR libraries have also been designed for other specific purposes, such as barcoding or targeting RNA.
When choosing your library, please be aware that some of the library vectors contain Cas enzyme (one-plasmid system), while others require co-infection with Cas enzyme-expressing virus or use in cells expressing Cas enzyme (two-plasmid system).
Click on different properties to create a custom filtered list of the CRISPR pooled libraries in Addgene's collection.
| ID | Name | Description | Library Type | Species | Genes Targeted | gRNAs per gene | Total gRNAs | PI | |
|---|---|---|---|---|---|---|---|---|---|
| 247028 | Mouse CRISPR Knockout Pooled Library (Julianna) | Enriched for effective guides through prioritization of guides with strong on-target activity. Designed against current gene annotations, offering coverage of the contemporary mouse genome. | Knockout | Mouse | 21,895 | 3 | 63,017 | John Doench, David Root | |
| 247026 247027 |
Human CRISPR Knockout Pooled Libraries (Jacquere) | Enriched for effective guides through prioritization of guides with strong on-target activity. Designed against current gene annotations, offering coverage of the contemporary human genome. | Knockout | Human | 20,550 | 3 | 60,550 | John Doench, David Root | |
| 234855 1000000254 |
sgRNA libraries for Pseudomonas aeruginosa PA14 | Inhibition library for inducible, genome-wide gene repression in Pseudomonas aeruginosa PA14. | Inhibition | P. aeruginosa | 5,981 5,971 |
1 | 5,981 5,971 |
Xue Liu | |
| 234819 234820 |
UMI-sgRNA Pooled Libraries | Knockout library targeting transcription factors (TF) or chromatin factors (CH) in mice. | Knockout | Mouse | 2,055 | 6 | 12,727 | Benjamin Ebert | |
| 232070 | CRASP-Seq BE Tiling Library | Tiling library to conduct CRASP-Seq experiments combined with high-throughput base editing for precise and scalable investigations into protein regions that affect splicing regulation. | Base Editing | Human | 39 | Varies | 8,594 | Thomas Gonatopoulos-Pournatzis | |
| 232069 | CRASP-Seq Gene KO Library | Knockout library for use in CRASP-Seq experiments. Provides a flexible platform for studying splicing regulation. | Knockout | Human | 22,987 | Varies | 95,893 | Thomas Gonatopoulos-Pournatzis | |
| 229137 229138 229139 229140 |
HR700_TP53 Exon Mutation Libraries | Donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of human TP53 exon 5–8. | Donor Vector | Human | 1 (4 exons) | Varies | 1,910–3,034 per exon | Thorsten Stiewe | |
| 227706 227707 227708 |
epegRNA Pooled Libraries | Assess prime editing-induced dropout phenotypes or prime editing efficiency in a pooled format. | Prime Editing | Human Human Mouse |
Varies | 1–20 per edit | Varies | Brittany Adamson | |
| 227014 | Human Cas13 lncRNA Library | CRISPR-Cas13 library targeting long noncoding RNAs (lncRNAs) that are highly expressed in human tissues for transcriptome-wide knockdown screens. | RNA Knockdown | Human | 6,199 lncRNAs, 4,390 genes | 6–8 | 75,184 | Neville Sanjana | |
| 226117 | Advanced Catalogue of Epigenetic Regulators (ACER) | Contains 748 traditional and newly predicted epigenetic regulators using epigenetic network analyses that integrated gene codependency (DepMap) and protein-protein interaction data (BioPlex). | Knockout | Human | 758 | 10 | 8,205 | Rui Lu | |
| 223064 | CROPseq-iT7 Human CRISPR Knockout Pooled Library (Brunello) | Knockout library to carry out genome-scale optical screening using Nuclear In-Situ Sequencing (NIS-Seq). | Knockout | Human | 19,114 | 4 | 76,441 | Jonathan Schmid-Burgk | |
| 217744 | TUNEYALI Library | Targets 56 transcription factors in Yarrowia lipolytica, enabling the knockout or modulation of expression levels for these factors. | Knockout | Yeast | 56 | 1 | 56 | Irina Borodina | |
| 216133 216134 |
Human Genome-wide Intron Tagging Libraries | Intron-targeting sgRNAs for pooled tagging of proteins with GFP or other protein tags at their endogenous genomic locus using an intron tagging approach. | Other | Human | 14,158 14,011 |
1–3 per intron | 90,657 72,580 |
Stefan Kubicek | |
| 213927 213928 |
Bovine CRISPR Knockout Libraries | Knockout libraries targeting the protein coding genes in the cattle genome. | Knockout | Cow | 21,165 | 4 on average | 94,000 | Wenfang Spring Tan | |
| 213695 221409 221410 |
Human Transporter Knockout Libraries | Knockout library targeting human transmembrane transporters. | Knockout | Human | 673 653 90 |
Varies | Varies | Giulio Superti-Furga | |
| 212966 | Pan-cancer CasRx lncRNA Albarossa Library | Genome-scale library for pan-cancer interrogation of lncRNA dependencies using CasRx. | Knockout | Human | 24,171 lncRNAs | Varies | 156,250 | Roland Rad | |
| 209734 209735 209736 237473 |
CHyMErA Cas12a Nuclease Optimization, Large-Scale Exon Deletion, and scCHyMErA-Seq Exon Deletion hgRNA Libraries | Combinatorial genome editing library uses hybrid guide RNAs fusing Cas9 and different Cas12a gRNAs to systematically identify phenotypically important exons on a large scale. | Knockout | Human | 844 844 2,095 161 |
1–10 | 18,000 18,000 300,000 1,066 |
Thomas Gonatopoulos-Pournatzis | |
| 207471 1000000228 |
FNLCR CRISPRa Cell Surface Libraries | Libraries targeting cell surface proteins and soluble factors for activation of gene expression. | Activation | Human | 6,081 7,030 |
5 | 30,868 35,517 |
Raj Chari | |
| 206248 206249 |
Mouse Lipid and Sphingolipid Metabolism Knockout Libraries | Libraries that target mouse lipid and sphingolipid metabolic genes and can be used to find lipid metabolic vulnerabilities in mouse cells. | Knockout | Mouse | 295 59 |
7–10 | 2,105 630 |
Kivanc Birsoy | |
| 197348 197349 |
Human CRISPRi-v2 Dual Guide Libraries | Dual sgRNA libraries containing a fixed element (non-targeting guide or EMC2 guide) and the well-established human genome-wide CRISPRi-v2 library in tandem. | Inhibition | Human | 18,905 | 5 | 103,269 | Rebecca Voorhees | |
| 192824 | Mouse Liver CRISPR Knockout Library | Knockout library targeting 13,189 of the 13,266 protein-coding genes expressed in the mouse liver | Knockout | Mouse | 13,189 | ~5 | 71,878 | Kristin Knouse | |
| 190744 | Mouse CRISPR Proximal Poly(A) Site Knockout Library | Targets proximal poly(A) signal sequences in genes identified to be differentially polyadenylated in the mouse B16-F10 melanoma system. | Knockout | Mouse | 144 proximal poly(A) sites | 8–10 pgRNAs | 1,718 | Robert Bradley | |
| 183825 | BLaER1 pgRNA Library | Paired gRNA library targeting genes related to B-cell to macrophage transdifferentiation. | Knockout | Human | 1,040 | 10 | 11,750 | Roderic Guigo | |
| 182133 | Pan-Druggable Cancer Library | Knockout library targeting cancer-related genes. | Knockout | Human | 66 | 1 | 74 | Prashant Mali | |
| 180272 | KSHV Tiling Library | Tiles the entire ~154 kb of the BAC16 Kaposi’s Sarcoma-associated Herpes Virus genome. A guide every ~8 bp allows pooled screening of the viral genome. | Knockout | KSHV | N/A | N/A | 24,000 | Britt Glaunsinger | |
| 179217 179218 179219 |
Dow Lab Base Editing Sensor Libraries | Base editor sgRNAs target frequently occurring cancer-associated mutations that can be modeled with cytosine base editing. | Base Editing | Human, Mouse | 462 | ~100 | Varies | Lukas Dow | |
| 177663 177664 177670 |
Adamson DNA Repair CRISPRi Libraries | Inhibition libraries targeting human genes involved in DNA repair and associated processes. Used for Repair-seq experiments to map DNA repair processes and identify improved genome editing strategies. | Inhibition | Human | 476 118 476 |
∼3 | 1,573 366 1,573 |
Brittany Adamson | |
| 174163 | BARBEKO sgRNA Library | Cytosine base editors for genome-scale knockout screens in human cells. Targets gene start codons, splice sites, or introduces premature termination codons. | Knockout, Base Editing | Human | 17,501 | 3 | 53,502 | Wensheng Wei | |
| 173195 | MYC-CRISPR Library | Knockout library targeting human E-boxes genome-wide. | Knockout | Human | 24,981 E-boxes | 1–7 per element | 46,354 | Agnieszka Dzikiewicz-Krawczyk | |
| 171172 | Berger Lab Human Paralog Knockout Library (pgPEN) | Induces knockouts of paralogous gene pairs. Plasmids express a pair of guide RNAs targeting either individual genes or a pair of paralogs. | Knockout | Human | 1,030 gene pairs | 8–16 | 33,170 | Alice Berger | |
| 170432 | sgRNA Pool for Streptococcus pneumoniae | Concise library targeting the whole genome of S. pneumoniae D39V with one sgRNA per operon or gene. | Knockout | S. pneumoniae | 2,111 genetic elements | 1 | 1,499 | Jan-Willem Veening | |
| 168791 | Turner Lab Human messenger-RBP sgRNA Library | Knockout library targeting human mRNA binding proteins with 10 sgRNAs/gene. | Knockout | Human | 725 | 10 | 8,260 | Martin Turner | |
| 164084 | Human mTORC1 Focused Library | Knockout library targeting human genes required for mTORC1 activation and inhibition. | Knockout | Human | 712 | 10 | 7,501 | David Sabatini | |
| 160132 160133 160134 160135 |
High Throughput PAM Determination Assay Libraries | Designed to characterize PAM requirements of Cas9 and Cas12a enzymes. | Other | Synthetic | N/A | N/A | N/A | Keith Joung, Benjamin Kleinstiver | |
| 159391 | Mouse Validation (mVAL) CRISPR Library | Targets 182 immune evasion genes. Designed for loss-of-function screening against genes shown to increase sensistivity or resistance of cancer cells to cytotoxic T lymphocyte-mediated toxicity. | Knockout | Mouse | 182 | 4 | 182 | Jason Moffat | |
| 155199 155200 |
CHyMErA Paralog & Dual-targeting hgRNA and Exon-deletion hgRNA pooled libraries | These libraries express hybrid Cas9-Cas12a gRNAs under a single promoter. Used to identify genetic interactions between paralogs and investigate alternative exon function. | Knockout | Human | 6,333 2,157 |
Varies | 92,746 91,948 |
Jason Moffat, Benjamin Blencowe | |
| 153101 153102 153103 153104 153105 153106 |
Borodina Yeast Transporter Libraries | Yeast knockout libraries targeting transporter genes. Five sub-pools target different groups of transporters and a sixth library targets all 361 genes. | Knockout | Yeast | Varies | 1 | Varies | Irina Borodina | |
| 140633 | Human Whole Genome sgRNA iBAR Library | Enables genome-wide CRISPR screening at high MOI through use of barcoded gRNA plasmids. | Knockout | Human | 19,210 | 3 | 58,630 | Wensheng Wei | |
| 138084 | Bradley Human CRISPR Poison Exon Knockout Library | Targets 3' splice sites with paired gRNAs. Induces skipping of poison cassette exons and matching upstream constitutive exons. Compare loss of a constitutive coding exon with loss of a poison exon. | Knockout | Human | 963 exons | ~9 pairs/exon | 9,508 | Robert Bradley | |
| 138015 | Mouse Cardiac Transcriptional Regulators CRISPR Knockout Library | AAV library targeting transcriptional regulators expressed in cardiomyocytes. | Knockout | Mouse | 2,444 | 6 | 14,671 | William Pu | |
| 137999 | Human Genome-wide Reduced Double-gRNA Library | Knockout library that delivers random pairs of optimised gRNAs, reducing screens scale with similar performance to larger libraries. | Knockout | Human | 19,657 | 3 | 59,576 | Leopold Parts | |
| 136478 136479 |
Human CRISPRi titration libraries | Enables titration of ~2,500 genes from normal expression level to completely knocked down. Available in large-scale or compact form. | Inhibition | Human | ~2,500 | 23–24 1–9 |
119,510 25,518 |
Jonathan Weissman | |
| 134582 134583 134584 |
Drosophila Cell CRISPR Knockout Library | Drosophilia library separated into three distinct, enriched sub-pools.Designed for long-term sgRNA delivery in Drosophila cell lines. | Knockout | Fly | 955 2979 9954 |
8 6 6 |
7,956 17,827 59,406 |
Norbert Perrimon | |
| 123361 | Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library | CRISPR-Cpf1/Cas12a double knockout library. Targets 26 genes, chosen from analysis of human metastasis cohorts. It can be used to interrogate the genetic interactions driving metastasis. | Knockout | Mouse | 26 | 4 | 11,934 arrays | Sidi Chen | |
| 113584 113585 |
Mouse Tumor Suppressor Gene CRISPR Knockout Library | AAV knockout library targeting mouse tumor suppressor genes (TSG). Each plasmid carries one TSG-targeting sgRNA and one p53-targeting sgRNA. Can be used to interrogate tumorigenesis in vivo. | Knockout | Mouse | 56 | 4 | 286 | Sidi Chen | |
| 113134 113135 113136 113137 113138 113146 113147 |
Chong Zhang E. coli Genome-wide Inhibition Library | Targets ~4,000 E. coli genes across five libraries defined by biological categories. Additional libraries include a negative control (NC) control library and a custom test library targeting 86 genes. | Inhibition | E. coli | Varies | 15 | Varies | Chong Zhang | |
| 92352 | Focused Ras Synthetic Lethal Human CRISPR Knockout Library | Focused gRNA library that targets synthetic lethal candidate and control genes with oncogenic Ras. | Knockout | Human | 132 | 50 | 6,661 | David Sabatini, Eric Lander | |
| 85968 | Perturb-seq Guide Barcodes (GBC) | Barcode library for cloning gRNA sublibraries for single cell CRISPR analysis. | Barcode | Human | N/A | N/A | N/A | Jonathan Weissman | |
| 82480 | CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library | This library is intended to be used as a technical tool for removal of mitochondrial DNA from mouse genomic DNA preparations. | Knockout | Mouse | 114 | N/A | 395 | Wei Xie | |
| 79153 | Cas13a/C2c2 Protospacer flanking site (PFS) Library | Library of protospacer flanking sites (PFS) inserted at the 5' end of the β-lactamase gene for screening PFS preference for Cas13a/C2c2. | Knockout | E. coli | N/A | N/A | N/A | Feng Zhang | |
| 64750 | Oxford Fly | Library designed for genomic targeting and screening in Drosophila cells. All possible gRNA target sites within the shared exonic regions on either DNA strand of the Drosophila genome were computed. | Knockout | D. melanogaster | 13,501 | 3 | 40,279 | Ji-Long Liu | |
| 159392 159393 |
Mouse Toronto KnockOut (mTKO) CRISPR Library | Targets mouse protein-coding genes with 4–5 gRNAs/gene. Library includes 418 control gRNAs. | Knockout | Mouse | 19,463 | ~5 | 19,463 | Jason Moffat | |
| 209551 209552 |
Inzolia Human CRISPR/Cas12a Multiplex Knockout | Knockout enAsCas12a gRNA library that targets single genes and paralog pairs, triples, and quads with arrays of four gRNAs. Can be used for assaying genetic interactions. | Knockout | Human | Varies | 1, 2, or 4 | 49,766 arrays | John Doench | |
| 1000000106 | Human CRISPR lncRNA Activation Pooled Library | The Synergistic Activation Mediator (SAM) is an engineered transcriptional activation complex for the transcriptional activation of lncRNAs. Includes library and activator plasmids. | Activation | Human | 10,504 | 10 | 96,458 | Feng Zhang | |
| 69763 | Human genome-wide library v1 | Used for genome scale lentiviral CRISPR screening. Target sites were chosen in a region close to the translation initiation site. | Knockout | Human | 20,121 | 4 | 77,406 | Haoquan Wu | |
| 1000000069 | Toronto KnockOut - Version 1 | Targets nearly all human protein-coding genes. Base library includes gRNAs with no predicted off-target sites. Supplemental library includes 6 additional gRNAs per gene. | Knockout | Human | 17,661 | 12 | 176,500 | Jason Moffat | |
| 80636 | Toxoplasma Knockout | Knockout library targeting 8,158 predicted protein-coding gene in Toxoplasma gondii. | Knockout | T. gondii | 8,158 | 10 | 80,158 | Sebastian Lourido | |
| 89640 | Human Paired-guide RNA (pgRNA) Library for Long Non-coding RNAs (lncRNAs) | Designed for genomic deletion screening of functional lncRNAs (long non-coding RNAs) using lentiviral pgRNAs (paired-guide RNAs). | Knockout | Human | 671 lncRNAs | Varies | 12,472 pairs | Wensheng Wei | |
| 1000000132 | Human CRISPR Knockout Library | Designed using metrics described in Xu et al. (2015), which maximize on-target cleavage effects, minimize potential off-target effects, andensure sequence fidelity with corresponding target loci. | Knockout | Human | 18,436 | 10 | 185,634 | Xiaole Shirley Liu | |
| 112200 | Human miRNA CRISPR Knockout Library | Targets 1,594 primary human miRNA genes. The library contains 4-5 gRNAs per gene and 1,000 non-targeting control gRNAs for a total of 8,382 gRNAs. | Knockout | Human | 1,594 | 4-5 | 8,382 | Ren-Jang Lin | |
| 115927 | Bikard Lab E. coli Genome-wide Inhibition Library v1 | ~93,000 gRNAs targeting random positions along the genome of E. coli MG1655 with a NGG PAM motif. Contains ~19 targets per gene and can be used with SpdCas9 to perform pooled CRISPRi screens. | Inhibition | E. coli | ~4,500 | 19 | 92,919 | David Bikard | |
| 119977 | Human lncRNA Splicing-targeting CRISPR Library | Designed for genomic deletion screening of functional lncRNAs by targeting their splice sites. | Knockout | Human | 10,996 lncRNAs | Varies | 126,773 | Wensheng Wei | |
| 122131 | mTF-CRISPRai Library | Increases transcriptional activation of genes encoding transcription factors in combination with dCas9 activation systems, such as the SunTag and the dCas9-VP64 systems. | Activation, Inhibition | Mouse | 2,428 | Varies | 93,107 | Stanley Qi | |
| 130630 | Mini-human AsCpf1-based Human Genome-wide Knockout Library | Knockout library targeting 16,977 genes. Each gene is targeted by an AsCpf1(AsCas12a)-based array containing 3–4 guides concatenated in one vector. | Knockout | Human | 16,977 | 3–4 | 17,032 arrays | Giulio Draetta | |
| 131625 | Bikard Lab EcoWG1: E. coli Genome-wide Inhibition Library v2 | Bacterial inhibition library targeting every small RNA and ORF of the E. coli MG1655 genome. This library has higher inhibition efficiency than the psgRNA library. | Inhibition | E. coli | 4,444 | ~5 | 21,417 | David Bikard | |
| 132561 | Human SLC Activation Library | Activation library targeting human SLC proteins. | Activation | Human | 388 | 6 | 3,342 | Giulio Superti-Furga | |
| 136986 | Human CRISPR Knockout Pooled Library (Gattinara) | Designed for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brunello human genome-wide library. | Knockout | Human | 19,993 | 2 | 40,964 | John Doench | |
| 136987 | Mouse CRISPR Knockout Pooled Library (Gouda) | Designed for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brie mouse genome-wide library. | Knockout | Mouse | 21,601 | 2 | 44,155 | John Doench | |
| 140961 | Neelamegham Human GlycoGene CRISPR Library | Knockout library targeting human genes involved in cellular glycosylation. | Knockout | Human | 347 | 10 | 3,637 | Sriram Neelamegham | |
| 141436 | Mouse SLC Knockout Library | Knockout library targeting mouse SLC proteins. | Knockout | Mouse | 378 | 6 | 2,384 | Giulio Superti-Furga | |
| 161769 | Ingolia lab S. cerevisiae CRISPRi v1 – barcodes 181005 | Tet-inducible inhibition library targeting the yeast genome with barcode linked guide RNAs. | Inhibition | Yeast | ~6,700 | ~6 | 44,580 | Nicholas Ingolia | |
| 161829 | Yeast Inducible CRISPRi Library – Ampl133 | Inducible genome-wide CRISPRi library targeting S. cerevisiae genes with 6-12 guide RNAs per gene. | Inhibition | Yeast | 6,685 | 6–12 | 51,681 | Saeed Tavazoie | |
| 163954 | Mycobacterium tuberculosis CRISPRi Library (RLC12) | Targets open reading frames and non-coding RNAs in M. tuberculosis. | Inhibition | M. tuberculosis | 4,052 | Varies | 96,700 | Jeremy Rock | |
| 163955 | Mycobacterium smegmatis CRISPRi Library (RLC11) | Targets open reading frames and non-coding RNAs in M. smegmatis. | Inhibition | M. smegmatis | 6,642 | Varies | 176,286 | Jeremy Rock | |
| 163966 | Mouse Metabolism Library | Knockout library targeting mouse metabolic genes. | Knockout | Mouse | 2,918 | ~6 | 18,343 | Alec Kimmelman | |
| 1000000173 | Mouse CRISPR Knockout Library in lentiCRISPR v2-Blast | Delivered as two half-libraries M1 and M2, the guide RNAs in this knockout library are designed to maximize on-target cleavage while minimizing off-target effects. | Knockout | Mouse | 18,741 | 10 | 185,836 | Xiaole Shirley Liu | |
| 169082 | Mouse messenger-RBP sgRNA Library | Retroviral knockout library targeting mouse mRNA binding proteins with 10 sgRNAs/gene. | Knockout | Mouse | 1,300 | 10 | 13,500 | Martin Turner | |
| 170511 | Mouse Cherry Brie Pooled Library | Genome-wide knockout library. It is identical to the Brie library #73633, replacing Puromycin resistance with mCherry. | Knockout | Mouse | 19,674 | ~4 | 78,637 | Pavel Tolar | |
| 171531 | Human UBDUB CRISPR Knockout Library | Knockout library targeting ubiquitination and de-ubiquitination complex genes. | Knockout | Human | 1,500 | ~6 | 9,274 | Chunliang Li | |
| 172650 172651 |
Broad GPP Humagne Set C and Humagne Set D | Knockout enAsCas12a libraries designed for assays with limited cell numbers. Both libraries target the same 19,755 genes and each gene has one construct with two sgRNAs. | Knockout | Human | 20,355 | 2 | 40,710 | John Doench, David Root | |
| 178284 | Green monkey (Chlorocebus sabaeus) sgRNA library | Knockout library targeting the green monkey (Chlorocebus sabaeus) genome. | Knockout | Green Monkey | 20,991 | 4 | 84,963 | John Doench, David Root | |
| 187080 | Human Metabolic Gene CRISPRa sgRNA Library | Library targeting 2,989 human metabolic genes for activation of gene expression. | Activation | Human | 2,989 | Varies | 32,460 | Kivanc Birsoy | |
| 187081 | Mouse Metabolic Gene CRISPRa sgRNA Library | Library targeting 2,989 mouse metabolic genes for activation of gene expression. | Activation | Mouse | 2,989 | Varies | 17,031 | Kivanc Birsoy | |
| 191535 | Human Lipid Droplet and Metabolism Library | Targets 1,196 genes related to lipid droplet biology and cell metabolism. | Knockout | Human | 1,196 | ~10 | 13,920 | James Olzmann | |
| 200011 | Mouse Chromatin Regulator Library | Knockout library targeting a comprehensive set of mouse genes with annotated function as chromatin regulators. | Knockout | Mouse | 936 | 6 | 5,616 | ||
| 83989 83990 83991 83992 83993 83994 83995 |
mCRISPRi-v2 subpooled libraries | Subpooled libraries targeting kinases, apoptosis, stress, trafficking, gene expression, membrane proteins, and unknown functions. dCas9-KRAB can be used as the transcriptional repressor. | Inhibition | Mouse | Varies | 5 | Varies | Jonathan Weissman | |
| 86538 86539 86540 86541 86542 86543 86544 86545 86546 86547 86548 86549 86550 |
CRiNCL - Human CRISPRi Non-coding Libraries | Targets long non-coding RNA (lncRNA)-expressing genes. dCas9-KRAB can be used as the transcriptional repressor. | Inhibition | Human | Varies | 10 | Varies | Jonathan Weissman | |
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|
187246 187247 187248 1000000192 |
Dual sgRNA CRISPRi Libraries | Ultra-compact inhibition library targeting each gene with a single element encoding a dual sgRNA cassette. | Inhibition | Human | Varies | 6 | Varies | Jonathan Weissman |
| 187249 187250 187251 1000000193 |
Dual sgRNA CRISPRa Libraries | Ultra-compact, human genome-wide activation library targeting each gene with a single element encoding a dual sgRNA cassette. | Activation | Human | Varies | 6 | Varies | Jonathan Weissman | |
| 200445 | Murine Cell Surface Protein Knockout (McspKO) Library | Knockout library targeting mouse cell surface genes. | Knockout | Mouse | 1,157 | 3–4 | 5,001 | Junjie Chen | |
| 75317 75316 |
Broad GPP kinome Brie | Knockout library that targets mouse kinome genes. | Knockout | Mouse | 713 | 4 | 2,852 | John Doench, David Root | |
| 1000000100 | Activity-optimized genome-wide library | Optimized for cleavage activity in order to maximize the likelihood of gene knockout. Split into three sub-libraries, each containing 10 gRNAs per gene. | Knockout | Human | 18,663 | 10 | 187,535 | David Sabatini, Eric Lander | |
| 1000000095 | Two plasmid human activity-optimized genome-wide library | Optimized for cleavage activity in order to maximize the likelihood of gene knockout. Split into three sub-libraries, each containing 10 gRNAs per gene. | Knockout | Human | 18,543 | 10 | 187,536 | David Sabatini, Eric Lander | |
| 1000000096 | Two plasmid mouse activity-optimized genome-wide library | Optimized for cleavage activity in order to maximize the likelihood of gene knockout. Split into two sub-libraries, each containing 10 gRNAs per gene. | Knockout | Mouse | 18,986 | 10 | 188,509 | David Sabatini, Eric Lander | |
|
|
104090 104091 1000000131 |
Broad GPP inhibition Dolomiti | Library for inhibition of over 22,000 mouse genes for genom-wide screening. Split into 2 pools targeting the same genes. Transcriptional repressor must be supplied separately. | Inhibition | Mouse | 22,311 22,247 |
3–6 | 67,366 67,194 |
John Doench, David Root |
| 117725 | Human Kinase Domain-Focused CRISPR Knockout Library | Designed for genomic deletion screening of protein kinases by targeting the kinase domains. | Knockout | Human | 482 | 6 | 3,051 | Christopher Vakoc | |
| 123334 | Human DNA Binding Domain-Focused CRISPR Knockout Library | Designed for genomic deletion screening of transcription factors by targeting the DNA binding domains. | Knockout | Human | 1,427 | 6 | 8,658 | Christopher Vakoc | |
| 125753 | Human Interferon-Stimulated Gene CRISPR Knockout Library | Knockout library targeting a set of human interferon-stimulated genes (ISGs). | Knockout | Human | 1,902 | 8 | 15,416 | Michael Emerman Molly OhAinle |
|
| 132552 | Human SLC Knockout Library | Knockout library targeting human SLC proteins. | Knockout | Human | 390 | 6 | 2,609 | Giulio Superti-Furga | |
| 140219 | DNA Damage Response MKOv4 Library | Knockout library targeting human DNA damage response and DNA repair genes. | Knockout | Human | 365 | 10 | 4,530 | Junjie Chen | |
| 164896 | Garnett Lab MinLibCas9 Library | Genome-wide knockout library designed with two sgRNAs per gene. | Knockout | Human | 18,761 | 2 | 37,722 | Mathew Garnett | |
| 174592 | Bonifacino Lab Human ubiquitination-related proteins CRISPR KO library | Targets human ubiquitation-related proteins and deubiquitinating enzymes. | Knockout | Human | 660 | 6–25 | 11,108 | Juan Bonifacino | |
| 200013 | The Human Druggable CRISPR Library | Knockout library containing ~10,000 sgRNAs targeting 1,980 human druggable genes (5 sgRNAs per gene and 100 non-targeting control sgRNAs). | Knockout | Human | 1,980 | 5 | 10,000 | Lianxin Liu | |
| 83971 83972 83973 83974 83975 83976 83977 |
hCRISPRi-v2 subpooled libraries | Subpooled libraries targeting kinases, apoptosis, stress, trafficking, gene expression, membrane proteins, and unknown functions. dCas9-KRAB can be used as the transcriptional repressor. | Inhibition | Human | Varies | 5 | Varies | Jonathan Weissman | |
| 83980 83981 83982 83983 83984 83985 83986 |
hCRISPRa-v2 subpooled libraries | SunTag-VP64 constructs can be used for activation and must be supplied separately. Subpools include kinases, apoptosis, stress, trafficking, gene expression, membrane proteins, and unknown function. | Activation | Human | Varies | 5 | Varies | Jonathan Weissman | |
| 83987 1000000092 |
CRISPRi-v2 | Genome-wide inhibition of gene transcription. dCas9-KRAB can be used as the transcriptional repressor. | Inhibition | Mouse | 20,003 | 5 10 |
107,415 214,830 |
Jonathan Weissman | |
| 83996 1000000093 |
CRISPRa-v2 | Increases transcriptional activation through the dCas9-SunTag-VP64 system, which enables specific recruitment of multiple copies of VP64 to the sgRNA-targeted gene. | Activation | Mouse | 19,939 | 5 10 |
107,105 214,210 |
Jonathan Weissman | |
| 83998 83999 84000 84001 84001 84002 84003 84004 |
mCRISPRa-v2 subpooled libraries | SunTag-VP64 constructs can be used for activation and must be supplied separately. Subpools include kinases, apoptosis, stress, trafficking, gene expression, membrane proteins, and unknown function. | Activation | Mouse | Varies | 5 | Varies | Jonathan Weissman | |
| 1000000121 1000000122 1000000123 1000000124 1000000125 1000000126 1000000127 1000000128 1000000129 1000000130 |
Bassik Mouse CRISPR Knockout Library | Targets all ~23,000 protein-coding genes in the mouse genome. It is divided into 10 sublibraries defined by biological categories, and each of which is split by gene into two sublibraries (A and B). | Knockout | Mouse | Varies (~23,000 in total) | 10 | Varies | Michael Bassik | |
| 1000000048 1000000049 |
Human GeCKO v2 | Genome-wide knockout library delivered as two half-libraries (A and B) allowing researchers to screen with 3 or 6 gRNAs/gene, respectively. | Knockout | Human | 19,052 | 6 | 123,411 | Feng Zhang | |
| 1000000052 1000000053 |
Mouse GeCKO v2 | Genome-wide knockout library delivered as two half-libraries (A and B) allowing researchers to screen with 3 or 6 gRNAs/gene, respectively. | Knockout | Mouse | 20,661 | 6 | 130,209 | Feng Zhang | |
| 73179 73178 |
Broad GPP genome-wide Brunello | Knockout library designed using optimized metrics which combined improved on-target activity predictions (Rule Set 2), with an off-target score, the Cutting Frequency Determination (CFD). | Knockout | Human | 19,114 | 4 | 76,441 | John Doench, David Root | |
| 73632 73633 |
Broad GPP genome-wide Brie | Knockout library designed using optimized metrics which combined improved on-target activity predictions (Rule Set 2), with an off-target score, the Cutting Frequency Determination (CFD). | Knockout | Mouse | 19,674 | 4 | 78,637 | John Doench, David Root | |
| 1000000057 1000000074 |
SAM v1 - 3 plasmid system | The Synergistic Activation Mediator (SAM) is an engineered transcriptional activation complex for the transcriptional activation of endogenous genes. Includes library and activator plasmids. | Activation | Human | 23,430 | 3 | 70,290 | Feng Zhang | |
| 1000000075 | SAM v1 - 3 plasmid system | The Synergistic Activation Mediator (SAM) is an engineered transcriptional activation complex for the transcriptional activation of endogenous genes. Includes library and activator plasmids. | Activation | Mouse | 23,439 | 3 | 69,716 | Feng Zhang | |
| 67988 | Mouse improved genome-wide library v2 | Knockout library targeting 18,424 mouse genes. gRNAs were improved using a new design pipeline and improved gRNA scaffold. | Knockout | Mouse | 18,424 | 5 | 90,230 | Kosuke Yusa | |
| 67989 | Human improved genome-wide library v1 | Knockout library targeting 18,010 human genes. gRNAs were improved using a new design pipeline and improved gRNA scaffold. | Knockout | Human | 18,010 | 5 | 90,709 | Kosuke Yusa | |
| 1000000078 | SAM v2 - 2 plasmid system | Version 2 of the human CRISPR/Cas9 SAM (Synergistic Activation Mediator) pooled library. Uses an engineered protein complex for the transcriptional activation of endogenous genes. | Activation | Human | 23,430 | 3 | 70,290 | Feng Zhang | |
|
|
92383 92384 1000000113 |
Broad GPP activation Caprano p65-HSF | Library that activates over 22,000 mouse genes and is used for genome-wide activation screening. Uses p65-HSF. | Activation | Mouse | 22,774 22,658 |
3–6 | 67,187 66,889 |
John Doench, David Root |
| 104861 | Retroviral Mouse Genome-wide CRISPR Knockout Library | Retroviral library containing gRNAs from the Yusa lab Mouse improved genome-wide library v2. | Knockout | Mouse | 18,424 | 5 | 90,230 | Sarah Teichmann | |
| 110066 | Human CRISPR Metabolic Gene Knockout Library | Targets 2,981 human metabolic genes with 29,790 guide RNAs. The library contains ~10 gRNAs per gene. | Knockout | Human | 2,981 | 10 | 30,290 | David Sabatini | |
| 113345 | Human Membrane Protein Activation Library | Activation library targeting the promoters of 6,213 genes that encode at least one putative membrane-associated protein isoform. | Activation | Human | 6,213 | 7–14 | 58,570 | Gavin Wright | |
| 141438 | RNA-Binding Protein Pooled CRISPR Knockout Library | Knockout library targeting RNA-binding proteins with 10 guide RNAs per target. | Knockout | Human | 1,078 | 10 | 12,472 | Eugene Yeo | |
| 160129 | Mouse CRISPR Metabolic Gene Knockout Library | Targets mouse metabolic genes and includes most metabolic enzymes and transporters. | Knockout | Mouse | 2,865 | 8 | 22,909 | Kivanc Birsoy | |
| 162256 | Human Epigenetic Knockout Library | Knockout library that targets human epigenetic genes. | Knockout | Human | 2,508 | 8 | 20,051 | Kivanc Birsoy | |
| 162275 | Human Transcription Factor Knockout Library | Knockout library targeting a set of human transcription factors for loss-of-function screening. | Knockout | Human | 1,639 | ~7 | 11,364 | Chunliang Li | |
| 169942 | Bison sgRNA Library | Knockout library targeting ubiquitin ligases, deubiquitinases, and control genes. | Knockout | Human | 713 | 4 | 2,852 | Benjamin Ebert | |
|
|
174196 174197 1000000178 |
MusCK Library | Delivered as two half-libraries Library A and Library B. The guide RNAs in these knockout libraries target mouse genes implicated in tumor initiation, progression, and immune modulation. | Knockout | Mouse | 4,922 | 5 5 10 |
49,252 | Xiaole Shirley Liu |
| 51043 51044 51045 51046 51047 51048 |
Human CRISPR Enriched Subpools (kinase, nuclear, ribosomal, cell cycle) Knockout Libraries | Knockout libraries containing 6 distinct subpools for targeting: kinase, cell cycle, nuclear, ribosomal, unknown, controls. | Knockout | Human | Varies | 10 | Varies | David Sabatini, Eric Lander | |
| 75314 75315 1000000083 75312 75313 1000000082 |
Broad GPP kinome Brunello | Knockout pooled library that targets kinome genes. Each half-library (1–4 or 5–8) contains 3,052 unique sgRNAs targeting 763 human kinase genes for 4 guides per target. | Knockout | Human | 763 | 4 | 3,052 | John Doench, David Root | |
| 83969 1000000090 |
CRISPRi-v2 | Genome-wide inhibition of gene transcription. dCas9-KRAB can be used as the transcriptional repressor. | Inhibition | Human | 18,905 | 5 10 |
104,535 209,070 |
Jonathan Weissman | |
| 83978 1000000091 |
CRISPRa-v2 | Increases transcriptional activation through the dCas9-SunTag-VP64 system, which enables specific recruitment of multiple copies of VP64 to the sgRNA-targeted gene. | Activation | Human | 18,915 | 5 10 |
104,540 209,080 |
Jonathan Weissman | |
| 90294 125517 |
Toronto KnockOut - Version 3 | Targets 18,053 protein coding genes (4 gRNAs/gene) with 142 control gRNAs targeting EGFP, LacZ, and luciferase. gRNAs are sequence-optimized based on the criteria described in the associated article. | Knockout | Human | 18,053 | 4 | 70,948 | Jason Moffat | |
| 101926 101927 101928 101929 101930 101931 101932 101933 101934 |
Bassik Human CRISPR Knockout Library | Targets all ~20,500 protein-coding genes in the human genome. It is divided into 9 sublibraries defined by biological categories of genes, each with their own negative controls. | Knockout | Human | Varies (~20,500 in total) | 10 | Varies | Michael Bassik | |
| 92385 92386 1000000114 |
Broad GPP inhibition Dolcetto | Library for inhibition of over 18,000 human genes for genom-wide screening. Split into 2 pools targeting the same genes. Transcriptional repressor must be supplied separately. | Inhibition | Human | 18,901 18,899 |
3–6 | 57,050 57,011 |
John Doench, David Root | |
| 92379 92380 1000000111 |
Broad GPP activation Calabrese p65-HSF | Library that activates over 18,000 human genes and is used for genome-wide activation screening. Uses p65-HSF. | Activation | Human | 18,885 18,843 |
3–6 | 56,762 56,476 |
John Doench, David Root | |
| 50947 | Mouse genome-wide library v1 (Discontinued) |
Library against 19,150 mouse protein coding genes, designed for use in lentivirus. | Knockout | Mouse | 19,150 | 5 | 87,897 | Kosuke Yusa | |
| 60956 | CRISPRa (Discontinued) |
Human genome-scale CRISPRa library for activation of gene transcription. | Activation | Human | 15,977 | 10 | 198,810 | Jonathan Weissman | |
| 62217 | CRISPRi (Discontinued) |
Library used for genome-wide inhibition of gene transcription. | Inhibition | Human | 15,977 | 10 | 206,421 | Jonathan Weissman | |
| 1000000067 | Activity-optimized genome-wide library (Discontinued) |
Optimized for cleavage activity, in order to maximize the likelihood of gene knockout. Split into two sub-libraries, each containing 5 sgRNAs per gene. | Knockout | Human | 18,166 | 10 | 178,896 | David Sabatini, Eric Lander |
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Screening Libraries
Screening libraries (non-CRISPR) can be used for different types of high-throughput experiments, including studying regulatory sequences, screening certain types of proteins (such as cell surface receptors or transcription factors), or observing the effects of mutagenesis of a single gene.
| ID | Library | Description | Species | Vector Type | Selection Marker | PI | |
|---|---|---|---|---|---|---|---|
| 1000000214 | APA Variant and Reference Libraries | Libraries made to experimentally validate a residual neural network model's (APARENT2) predictions for 3′-cleavage and polyadenylation from DNA sequence. | Human | Mammalian Expression | N/A | Georg Seelig | |
| 243681 243682 |
attB GPCR Libraries | Libraries for stable recombination of nearly all human human G protein-coupled receptors (GPCRs) with unique molecular identifiers (UMIs). | Human | Cloning | GFP | Jonathan Schlebach | |
| 172109 | Cancer Driver Peptide Overexpression Library | Library expressing tiled cancer driver peptides for fitness screening. | Human | Lentiviral | Puromycin | Prashant Mali | |
| 208054 | Cultivarium MACKEREL Library | Golden Gate compatible library to study a broad host range of prokaryotic promoters-RBS regulatory sequences. | Bacteria | Bacterial Expression | Chloramphenicol | Nili Ostrov, Charlie Gilbert, Cultivarium Tools | |
| 1000000194 1000000195 1000000196 |
DHFR Saturation Mutagenesis Libraries | Libraries to study the interaction between dihydrofolate reductase (DHFR) and thymidylate synthase (TYMS), a pair of enzymes catalyzing consecutive reactions in folate metabolism. | E. coli | Bacterial Expression | Chloramphenicol | Kimberly Reynolds | |
| 160132 160133 160134 160135 |
High Throughput PAM Determination Assay Libraries | Libraries for determining the PAM requirements of Cas enzymes. | Synthetic | Reporter | Ampicillin | Keith Joung, Benjamin Kleinstiver | |
| 224585 | Ma-PylRS-lib1 Library | Pyrrolysine tRNA synthetase library to generate new genetic code expansion (GCE) systems for site-specific incorporation of non-canonical amino acids (ncAAs) into proteins. | M. alvus | Bacterial Expression | Kanamycin | Ryan Mehl | |
201012 |
microTRE Screening Library | Libraries to screen synthetic promoters for transcriptional readouts of specific upstream signaling events. | Synthetic, but based on human and mouse | Reporter | N/A | Justin English | |
| 207474 207475 207476 207477 207478 207479 207480 207481 |
Modular Pooled Knockin Libraries | Libraries for screening transcription factors and cell surface receptors with a T cell receptor or chimeric antigen receptor for engineering immunotherapies and other cellular functions. | Human | Cloning/HDRT Generation | N/A | Alexander Marson | |
| 198050 | pCMV-Rep78/68 Scanning Saturation Mutagenesis (SSM) Library | Investigate the effects of rep mutations on production of a wide range of AAV capsid serotypes. | AAV2 | AAV | N/A | George Church | |
| 199601 | pRep-Cap Scanning Saturation Mutagenesis (SSM) Library | Investigate the effects of cap gene mutations on AAV production and on capsid properties. | AAV2 | AAV | N/A | George Church | |
| 1000000181 | PYR1 Double Mutant Library | Paired double mutant Pyrabactin Resistance 1 libraries. | A. thaliana | Yeast Expression | TRP1 | Timothy Whitehead | |
| 241288 241289 |
PYR1 Double-Hao and Triple Mutant Libraries | PYR1 receptor libraries to isolate sensors using growth-based selections in yeast. | Arabidopsis | Yeast Two Hybrid | TRP1 | Cutler | |
111704 |
Rinehart Human Serine Phosphopeptide Library | Human phosphoserine libraries. | Human | Bacterial Expression | Ampicillin | Jesse Rinehart | |
| 188526 188527 188528 188529 188530 188531 188532 188533 188534 188535 188536 1000000199 1000000200 1000000201 1000000202 1000000203 1000000204 1000000205 1000000206 1000000207 1000000208 1000000209 |
Rinehart Lab Iterative Synthetically Phosphorylated Isomers (iSPI) Library | New generation of human phosphoserine libraries. | Human | Bacterial Expression | Ampicillin | Jesse Rinehart | |
| 127842 | SPECS Library | Library for high-throughput screening for identification of synthetic promoters with enhanced cell-state specificity. | Synthetic | Lentiviral | N/A | Timothy Lu | |
| 213694 | Superti-Furga Human SLC Overexpression Library | An inducible barcoded overexpression library to test the impact of SLC transporters on biological processes. | Human | Lentiviral | Puromycin | Giulio Superti-Furga | |
| 1000000264 | TCR Rapid Assembly for Functional Testing (TCRAFT) Libraries | Use TCRAFT libraries for functional screening. | Human | Cloning | N/A | Michael Birnbaum | |
| 113569 | TP53 Mutagenesis Library | Designed for the characterization of all possible p53 mutations. | Human | Lentiviral | Puromycin | William Hahn, David Root | |
| 137000 192821 1000000218 |
Zhang Lab Multiplexed Overexpression of Regulatory Factors (MORF) Library | This pooled library of lentiviral plasmids expresses barcoded isoforms of human transcription factors. | Human | Lentiviral | Puromycin | Feng Zhang | |
| 79153 | Cas13a/C2c2 Protospacer flanking site (PFS) Library | A protospacer flanking site (PFS) library that can be used to evaluate Cas13a binding. | E. coli | Bacterial Expression | N/A | Feng Zhang |
Surface Display Libraries
Surface display is a genetic engineering technique that physically links a protein's function (phenotype) to the gene that encodes it (genotype). This is achieved by fusing the gene of interest with a gene for an anchoring protein. The resulting fusion protein is then expressed on the surface of a host cell or virus, such as bacteriophages or yeast.
The key advantage of this approach is the ability to screen large, diverse libraries for specific binding properties without the need to purify individual proteins. Any successful candidate can be readily identified by recovering and sequencing its associated genetic material.
Commonly used surface display platforms include:
- Phage Display: In this system, a peptide or protein is fused to a bacteriophage coat protein. The resulting phages can be screened for binding affinity to a target molecule.
- Yeast Display: In this system, proteins are displayed on the surface of Saccharomyces cerevisiae, often by fusion to the Aga2p protein. This platform supports the correct folding and post-translational modification of complex eukaryotic proteins, such as antibodies.
| ID | Library | Description | Species | Backbone Vector | PI | |
|---|---|---|---|---|---|---|
| 1000000071 | Synuclein VHH Immune Library | Created for research in Parkinson's disease; for target validation and drug design | Camelid | Phagemid | Anne Messer, MJFF | |
| 157971 157972 157973 168776 168777 174293 174294 174295 174296 |
SARS-CoV-2 Spike (S) Ectodomain and RBD Libraries | Libraries of Spike (S) Ectodomain and RBD mutants. | Coronavirus | Yeast surface display | Timothy Whitehead | |
| 1000000172 | SARS-CoV-2 Spike Receptor Binding Domain Deep Mutation Scanning Library | Library of Spike protein RBD mutations for comprehensive mapping. | Coronavirus | Yeast surface display | Jesse Bloom | |
| 1000000182 1000000183 1000000184 1000000185 1000000186 1000000187 1000000188 |
SARS-CoV-2 Spike Receptor Binding Domain Site-Saturation Mutagenesis Library | Libraries of variant Spike protein RBD mutations for comprehensive mapping. | Coronavirus | Yeast surface display | Jesse Bloom |
cDNA Libraries
cDNA libraries are created from an organism’s actively transcribed mRNA, as opposed to genomic libraries, which are created from genomic DNA. Since cDNA libraries lack introns and other nontranscribed sequences, they are smaller and easier to use than genomic libraries, but they do not contain as much regulatory information. To make a cDNA library, cDNA is synthesized, ligated to adapters, and then cloned into a given vector.
| ID | Library | Description | Species | PI | |
|---|---|---|---|---|---|
| 62843 | S. rosetta Col- cDNA amplified library | Created from the choanoflagellate Salpingoeca rosetta. Col- refers to DNA isolated from bacteria cultured with the colony-inducing bacteria Algoriphagus machipongonensis. | Salpingoeca rosetta | Nicole King | |
| 62844 | S. rosetta Col+ cDNA amplified library | Created from the choanoflagellate Salpingoeca rosetta. The Col+ library was created from S. rosetta cultured without bacteria. | Salpingoeca rosetta | Nicole King | |
| 25864 | Monosiga cDNA Library (Discontinued) |
Bacterial expression cDNA library created from the choanoflagellate Monosiga brevicollis. | Monosiga brevicollis | Sean Carroll, Nicole King | |
| 25716 | Proterospongia cDNA library (Discontinued) |
Bacterial expression cDNA library created from Proterospongia, also referred to as Salpingoeca. | Proterospongia | Nicole King |
shRNA Libraries
Addgene currently does not distribute any shRNA libraries. DECIPHER libraries below were previously distributed by Addgene and have been discontinued as of October 2016, but are noted here for historical reference.
| ID | Library | Species | Vector Type | Targets | Total shRNAs | PI | |
|---|---|---|---|---|---|---|---|
| 29589 | DECIPHER Mod 3: Cell Surface, etc. (Discontinued) |
Human | Lentiviral | 4,922 | 27,500 | Alex Chenchik | |
|
|
28285 28286 |
DECIPHER Mod 1: Pathway Targets (Discontinued) DECIPHER Mod 2: Disease Targets (Discontinued) |
Human | Lentiviral | 5,043 5,412 |
27,500 | Alex Chenchik, Gus Frango |
|
|
28287 28288 |
DECIPHER Mod 1: Pathway Targets (Discontinued) DECIPHER Mod 2: Disease Targets (Discontinued) |
Mouse | Lentiviral | 4,625 4,520 |
27,500 | Alex Chenchik, Gus Frango |
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Content last reviewed: 12 November 2025
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