(Pooled Library #178943)
This piggyBac barcoding library is a component of the macsGESTALT inducible lineage recording system. The plasmid contains both a static barcode (which is a random 10-mer ‘library’ here) and an evolving barcode (which is edited by CRISPR/Cas9 components delivered by other plasmids).
A companion plasmid, pUltra-U6-gRNAs1-5 (Addgene #178569), includes a gRNA-tRNA array for macsGESTALT editing.
PB-CMV-MCS-EF1a-Puro - does not express Cas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||178943||PB-EF1a-Puro-V8.2†||1||$400||Add to Cart|
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with Lenti-iCas9-neo (Addgene #85400) or any other plasmid(s) or cell lines expressing Cas9.
See publication for more information.
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
Lineage processing and analysis workflow is available on GitHub.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:macsGESTALT Library was a gift from Christopher Lengner (Addgene #178943)
For your References section:Single-cell lineage tracing of metastatic cancer reveals selection of hybrid EMT states. Simeonov KP, Byrns CN, Clark ML, Norgard RJ, Martin B, Stanger BZ, Shendure J, McKenna A, Lengner CJ. Cancer Cell. 2021 Aug 9;39(8):1150-1162.e9. doi: 10.1016/j.ccell.2021.05.005. PubMed 34115987