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macsGESTALT Library
(Pooled Library #178943)

  • Purpose

    This piggyBac barcoding library is a component of the macsGESTALT inducible lineage recording system. The plasmid contains both a static barcode (which is a random 10-mer ‘library’ here) and an evolving barcode (which is edited by CRISPR/Cas9 components delivered by other plasmids).

    A companion plasmid, pUltra-U6-gRNAs1-5 (Addgene #178569, ordered separately), includes a gRNA-tRNA array for macsGESTALT editing.

  • Vector Backbone

    PB-CMV-MCS-EF1a-Puro - does not express Cas9


Item Catalog # Description Quantity Price (USD)
Pooled Library 178943 PB-EF1a-Puro-V8.2 1 Pending
Available to Academic and Nonprofits Only
  • pUltra-U6-gRNAs1-5 (Addgene #178569) and a Cas9 plasmid are NOT included with this item and will have to be ordered separately. The library can be used in conjunction with Lenti-iCas9-neo (Addgene #85400) or any other plasmid(s) or cell lines expressing Cas9.

Library Details

See publication for more information.

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼10 µL
  • Concentration
    25 ng/µL

Resource Information

Depositor Comments

Lineage processing and analysis workflow is available on GitHub.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    macsGESTALT Library was a gift from Christopher Lengner (Addgene #178943)
  • For your References section:

    Single-cell lineage tracing of metastatic cancer reveals selection of hybrid EMT states. Simeonov KP, Byrns CN, Clark ML, Norgard RJ, Martin B, Stanger BZ, Shendure J, McKenna A, Lengner CJ. Cancer Cell. 2021 Aug 9;39(8):1150-1162.e9. doi: 10.1016/j.ccell.2021.05.005. PubMed 34115987