Multiplexable, locus-specific targeting of long RNAs with CRISPR-Display.
Shechner DM, Hacisuleyman E, Younger ST, Rinn JL
Nat Methods. 2015 Jul;12(7):664-70. doi: 10.1038/nmeth.3433. Epub 2015 Jun 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 68414 | pLenti_9xD3B_Cluc_Norm | CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," lentiviral format. |
| 68415 | pTrans_9xD3B_Cluc_Norm | CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format. |
| 68416 | pEF_dCas9 | Transient expression of Sp-dCas9 in mammalian cells, under an EF1-alpha promoter. |
| 68417 | pEF_dCas9-VP64 | Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter. |
| 68418 | pUBC_HA_2xPP7-VP64 | Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter |
| 68419 | pCMV_mCherry_2A_HA_2xMS2-VP64 | Transient expression of humanized MS2 (V75E/A81G), fused to the VP64 transcrption activator, in mammalian cells, under a CMV promoter |
| 68420 | pUBC_V5_MS2-mCherry | Transient expression of humanized MS2 (V75E/A81G), fused to mCherry in mammalian cells, under a UBC promoter |
| 68421 | pUBC_HA_L7Ae-VP64 | Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter |
| 68422 | pU6_(GLuc)_sgRNA | Transient expression of a minimal sgRNA targeting the GLuc reporter, in mammalian cells. U6 promoter |
| 68423 | pU6_(GLuc)_TOP1 | Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U6 promoter |
| 68424 | pU6_(GLuc)_INT[3xPP7_SL] | Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U6 promoter |
| 68425 | pU6_(GLuc)_INT[S1Apt] | Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter |
| 68426 | pU6_(GLuc)_INT[3xMS2_SL] | Transient expression in mammalian cells of an "INT" construct_bearing three MS2 Stem-loops, targeting the GLuc reporter. U6 promoter |
| 68428 | pU6_(GLuc)_INT[GFPApt] | Transient expression in mammalian cells of an "INT" construct_bearing the GFP aptamer, targeting the GLuc reporter. U6 promoter |
| 68429 | pU6_(GLuc)_INT[3xK-T] | Transient expression in mammalian cells of an "INT" construct_bearing three kink-turns, targeting the GLuc reporter. U6 promoter |
| 68430 | pU6_(GLuc)_INT[Spinach2] | Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter |
| 68431 | pU6_(GLuc)_INT[BoBS] | Transient expression in mammalian cells of an "INT" construct_bearing the "Bunch of Baby Spinach" (or "BoBS") construct, targeting the GLuc reporter. U6 promoter |
| 68432 | pU6_(GLuc)_INT[P4-P6(3xPP7)] | Transient expression in mammalian cells of an "INT" construct_bearing P4_P6 (internally appended with three PP7 stem-loops), targeting the GLuc reporter. U6 promoter |
| 68433 | pU6_(Gluc)_INT(GenPurpClon) | General Purpose cloning vector for "INT"-like constructs under U6-promoter expression. |
| 68434 | pCMV/3' Box_(GLuc)_TOP1 | Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone. |
| 68435 | pCMV/3' Box_(GLuc)_TOP2 | Transient expression of the "TOP2" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone. |
| 68436 | pCMV/3' Box_(GLuc)_INT | Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone. |
| 68437 | pU1/Sm/3' Box_(GLuc)_TOP1 | Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone. |
| 68438 | pU1/Sm/3' Box_(GLuc)_INT | Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone. |
| 68439 | pCMV/MASC_(GLuc)_TOP1 | Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/MASC expression backbone. |
| 68440 | pCMV/MASC_(GLuc)_INT | Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/ MASC expression backbone. |