Highly cooperative chimeric super-SOX induces naive pluripotency across species.
MacCarthy CM, Wu G, Malik V, Menuchin-Lasowski Y, Velychko T, Keshet G, Fan R, Bedzhov I, Church GM, Jauch R, Cojocaru V, Scholer HR, Velychko S
Cell Stem Cell. 2024 Jan 4;31(1):127-147.e9. doi: 10.1016/j.stem.2023.11.010. Epub 2023 Dec 22.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 193290 | pCXLE-SOX2-17-p2a-KLF4 | episomal hS*K (expressing human super-SOX + KLF4) for enhanced generation of intergration-free iPSCs or for integration-free naïve conversion |
| 193291 | pCXLE-SOX2AV-p2a-KLF4 | episomal expressing human SOX2A61V + KLF4 |
| 193292 | pCXLE-SOX2-p2a-KLF4 | episomal hSK (expressing human SOX2 + KLF4) |
| 193293 | pCXLE-mCherry | episomal vector expressing mCherry fluorescent protein |
| 193294 | pCXLE-mCherry-t2a-SOX2-17-p2a-KLF4 | episomal mCherry-hS*K (expressing mCherry + human super-SOX + KLF4) for integration-free naïve conversion |
| 193295 | pCXLE-mCherry-t2a-SOX2AV-p2a-KLF4 | episomal vector expressing mCherry + human SOX2A61V + KLF4 |
| 193296 | pCXLE-mCherry-t2a-SOX2-p2a-KLF4 | episomal mCherry-hSK (expressing mCherry + human SOX2 + KLF4) |
| 193298 | pCXLE-Oct4-p2a-Klf4-IRES-Sox2-17 | episomal OKS* (expressing mouse Oct4 + Klf4 + super-Sox) for generation of integration-free iPSCs |
| 193299 | pHAGE2-TetOminiCMV-Klf4-IRES-Sox2-17 | tetO-KS* (Dox-inducible reprogramming lentiviral vector expressing mouse Klf4 + super-Sox) |
| 193345 | pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4 | tetO-OS*K (Dox-inducible reprogramming lentiviral OSK vector expressing mouse Oct4 + super-Sox + Klf4) |
| 193346 | pHAGE2-TetOminiCMV-Oct4-p2a-Sox2-17-t2a-Klf4-e2a-cMyc | tetO-OS*KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + super-Sox + Klf4 + cMyc) |
| 193347 | pHAGE2-TetOminiCMV-Oct4-p2a-Sox2AV-t2a-Klf4-e2a-Myc | tetO-Sox2AV-KM (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Sox2A61V + Klf4 + cMyc) |
| 193348 | pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4 | tetO-S*K (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4) |
| 193349 | pHAGE2-TetOminiCMV-Sox2-17-t2a-Klf4-e2a-Myc | tetO-S*KM (Dox-inducible reprogramming lentiviral vector expressing mouse super-Sox + Klf4 + cMyc) |
| 193350 | pMXs-hSOX2-17 | Retroviral vector expressing human SOX2-17 chimeric transcription factor (super-SOX) |
| 193351 | pMXs-hSOX2 | Retroviral vector expressing human SOX2 |
| 193352 | pMXs-hSOX2AV | Retroviral vector expressing human SOX2A61V point mutant |
| 193353 | pMXs-Sox2-17 | Retroviral vector expressing mouse Sox2-17 chimeric transcription factor (super-Sox) |
| 193354 | pMXs-Sox2AV | Retroviral vector expressing mouse Sox2A61V point mutant |
| 193355 | T7-VEE-mCherry | Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR |
| 193356 | T7-VEE-OCT4-t2a-KLF4-e2a-SOX2-17-IRES-GLIS1 | VEE-OKS*iG (self-replicating RNA vector expressing human OCT4 + KLF4 + super-SOX + GLIS1 + PuroR) for generation of integration-free iPSCs |
| 193357 | T7-VEE-CFP | Self-replicating RNA vector expressing CFP fluorescent protein + PuroR |
| 193358 | T7-VEE-CFP-e2a-SOX2-p2a-KLF4 | VEE-CFP-SK (self-replicating RNA vector expressing CFP + human SOX2 + KLF4 + PuroR) |
| 193359 | T7-VEE-CFP-e2a-SOX2AV-p2a-KLF4 | self-replicating RNA vector expressing CFP + human SOX2A61V + KLF4 + PuroR |
| 193360 | T7-VEE-CFP-e2a-SOX2-17-p2a-KLF4 | VEE-CFP-S*K (self-replicating RNA vector expressing CFP + human super-SOX + KLF4 + PuroR) for integration-free naïve conversion |
| 210016 | pCXLE-Sox2-17-T2A-Klf4-E2A-Myc | episomal S*KM (expressing mouse super-Sox + Klf4 + Myc) for integration-free naïve reset |
| 210017 | pCXLE-Sox2AV-T2A-Klf4-E2A-Myc | episomal SKM (expressing mouse Sox2-A61V + Klf4 + Myc) for integration-free naïve reset. |
| 210018 | pCXLE-Sox2-T2A-Klf4-E2A-Myc | episomal SKM (expressing mouse Sox2 + Klf4 + Myc) for integration-free naïve reset |
| 210019 | pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2 | tetO-OKS (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + Sox2) |
| 210020 | pHAGE2-TetOminiCMV-Oct4-P2A-Klf4-IRES-Sox2-17 | tetO-OKS* (Dox-inducible reprogramming lentiviral vector expressing mouse Oct4 + Klf4 + super-Sox) |