Alzheimer disease-associated tau post-translational modification mimics impact tau propagation and uptake.
Dickson JR, Sobolewski RGR, Fernandes AR, Cooper JM, Fan Z, Chung M, Donahue C, Oakley DH, Strickland DK, Hyman BT
J Neuropathol Exp Neurol. 2025 Jun 1;84(6):459-470. doi: 10.1093/jnen/nlaf007.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 226380 | pcDNA3.1(-).TagRFP.T-2A-Tau2N4R-WPRE | Expresses tau propagation ORF with no V5 tag on WT 2N4R tau |
| 226381 | pcDNA3.1(-).TagRFP.T-2A-V5-Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on WT 2N4R tau |
| 226382 | pcDNA3.1(-).TagRFP.T-2A-V5-8D2Q.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on 8D2Q mutant 2N4R tau |
| 226383 | pcDNA3.1(-).TagRFP.T-2A-V5-20D3Q.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on 20D3Q mutant 2N4R tau |
| 226384 | pcDNA3.1(-).TagRFP.T-2A-V5-8A2R.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on 8A2R mutant 2N4R tau |
| 226385 | pcDNA3.1(-).TagRFP.T-2A-V5-20A3R.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on 20A3R mutant 2N4R tau |
| 226386 | pcDNA3.1(-).TagRFP.T-2A-V5-K281Q.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on K281Q mutant 2N4R tau |
| 226387 | pcDNA3.1(-).TagRFP.T-2A-V5-K353Q.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on K353Q mutant 2N4R tau |
| 226388 | pcDNA3.1(-).TagRFP.T-2A-V5-S198D.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on S189D mutant 2N4R tau |
| 226389 | pcDNA3.1(-).TagRFP.T-2A-V5-S199D.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on S199D mutant 2N4R tau |
| 226390 | pcDNA3.1(-).TagRFP.T-2A-V5-S202D.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on S202D mutant 2N4R tau |
| 226391 | pcDNA3.1(-).TagRFP.T-2A-V5-S404D.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on S404D mutant 2N4R tau |
| 226392 | pcDNA3.1(-).TagRFP.T-2A-V5-T217D.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on T217D mutant 2N4R tau |
| 226393 | pcDNA3.1(-).TagRFP.T-2A-V5-T403D.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on T403D mutant 2N4R tau |
| 226394 | pcDNA3.1(-).TagRFP.T-2A-V5-T181D.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on T181D mutant 2N4R tau |
| 226395 | pcDNA3.1(-).TagRFP.T-2A-V5-T231D.Tau2N4R-WPRE | Expresses tau propagation ORF with V5 tag on T231D mutant 2N4R tau |
| 226396 | pET28.His.3C.Tau2N4R | Expresses his-tagged, 3C-cleavable WT 2N4R tau for recombinant protein production in bacteria |
| 226397 | pET28.His.3C.20D3Q.Tau2N4R | Expresses his-tagged, 3C-cleavable 20D3Q mutant 2N4R tau for recombinant protein production in bacteria |
| 226398 | pET28.His.3C.8D2Q.Tau2N4R | Expresses his-tagged, 3C-cleavable 8D2Q mutant 2N4R tau for recombinant protein production in bacteria |
| 226399 | pET28.His.3C.86b.Tau2N4R | Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged WT 2N4R tau for recombinant protein production in bacteria |
| 226400 | pET28.His.3C.86b.8D2Q.Tau2N4R | Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 8D2Q mutant 2N4R tau for recombinant protein production in bacteria |
| 226401 | pET28.His.3C.86b.20D3Q.Tau2N4R | Expresses his-tagged, 3C-cleavable, 86b (split luciferase fragment)-tagged 20D3Q mutant 2N4R tau for recombinant protein production in bacteria |