Leveraging CRISPR activation for rapid assessment of gene editing products in human pluripotent stem cells
Wu Y, Zhong A, Evangelisti A, Sidharta M, Danwei H, Studer L, Zhou T
Stem Cell Reports, Volume 0, Issue 0, 102499
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 235593 | EF1a-TET1-dCas9 | Tet1CD fused to N-terminus of dCas9 |
| 235594 | EF1a-MVPH | Expresses the MS2-VP64-P65-HSF1 under the control of human EF1a promoter |
| 235595 | EF1a-dCas9-VP64 | Expresses the dCas9-VP64 under the control of human EF1a promoter |
| 235596 | EF1a-MPH | Expresses the MS2-P65-HSF1 under the control of human EF1a promoter |
| 235597 | LsgRNA-MS2 | sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. The cloning site is BsmbI. |
| 235600 | pLV-MVPH | Expresses the MS2-VP64-P65-HSF1 fusion protein |