Constitutive Gs activation using a single-construct tetracycline-inducible expression system in embryonic stem cells and mice.
Hsiao EC, Nguyen TD, Ng JK, Scott MJ, Chang WC, Zahed H, Conklin BR
Stem Cell Res Ther. 2011 Mar 4. 2(2):11.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 24414 | pEnt L1L3 tTA-2 | |
| 24415 | pEnt L1L3 EF1a-tTA-3 | |
| 24416 | pEnt R3L2 TetO(fl)-2 | |
| 24417 | pEnt R3L2 TetO(sh) mCh-Rs1-2 | Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Plasmid also known as pEntR3L2 TetO-mCh-Rs1. |
| 24418 | pRosa26 R1-ccdB-R2 RexNeo PI-SceI | |
| 24419 | Exp-Rosa-EF1a-tTA-mCh-Rs1-TetO(sh)-2 | Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Expresses mCherry and Rs1 via P2A ribosomal skip sequence. Expresses tTA under EF1α promoter. Rosa26 homology arms for integration. |
| 26797 | Exp-pcDNA3.2delCMV(EF1α-tTA/TetO-mCh-Rs1) | Human 5HT4B receptor with D100A mutation, generating the RASSL Rs1. Construct expresses both mCherry and Rs1 via a P2A ribosomal skip sequence. Expresses tTA under EF1α promoter separately. |
| 26798 | pEnt L1L3-MCS | |
| 26799 | pEnt R3L2-MCS | |
| 26803 | pEnt L1L3 EF1a-tTA-2 | |
| 27105 | pEnt L1L3 tTA-3 | |
| 27106 | pEnt L1L3 rtTA-3 | |
| 27107 | pEnt R3L2 TetO(fl)-3 | |
| 29496 | pcDNA3.2 GW delCMV |