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CRISPR-Cas/RGN expression plasmids for EGFP

The Joung Lab recently described the use CRISPR-Cas/RNA-Guided Nuclease (RGN) technology to modify three different sites in the EGFP reporter gene (Fu et al., Nat Biotechnol. 2013).

The expression plasmids encoding the guide RNAs (gRNAs) used in these experiments are listed below. Note that gRNA produced from each of these vectors were used in conjunction with mRNA produced from the mammalian codon-optimized Cas9 expression vector (JDS246). Additional details can be found in Fu et al., Nat Biotechnol. 2013 and users are encouraged to post questions to the Genome Engineering newsgroup (which can be joined here).

Individual plasmids can be ordered via the links below:

ID Plasmid
47511 pFYF1320 EGFP Site#1
47512 pFYF1320 EGFP Site#2
47513 pFYF1320 EGFP Site#3