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Mouse CRISPR Knockout Pooled Library (Julianna)
(Pooled Library #247028)

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  • Purpose

    This is a mouse genome-wide CRISPR knockout library for the use of Streptococcus pyogenes Cas9. Each gene is targeted by three constructs.

    The Julianna library is enriched for effective guides through prioritization of guides with strong on-target activity as predicted with the Rule Set 3 Sequence + Target Score (DeWeirdt et al., 2022 (Link opens in a new window)) and strategic exclusion of guides with excessive off-target activity. Further, Julianna is designed against current gene annotations sourced from Ensembl v102, thereby offering coverage of the contemporary mouse genome.

  • Vector Backbone

    pMV_AA050 (Plasmid #216107) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 247028 Mouse CRISPR KO Library† 1 $594 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pMV_AA088 (Addgene #216104) or any other plasmid(s) or cell lines expressing Cas9.

Library Details

  • Species
    Mouse (GRCm38)
  • Genes targeted
    21,895
  • gRNAs per gene
    3
  • Total gRNAs
    63,017
  • Controls
    900 intergenic-targeting, 100 non-targeting
  • Lentiviral generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼25 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

Deconvolution algorithms for analyzing NGS data: Extract guide sequences from sequencing reads by running PoolQ with the search prefix “CACCG”, and count reads by alignment to a reference file of all possible guides present in the library

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse CRISPR Knockout Pooled Library (Julianna) was a gift from John Doench and David Root (Addgene #247028 ; http://n2t.net/addgene:247028 ; RRID:Addgene_247028)

  • For your References section:

    Balancing off-target and on-target considerations for optimized Cas9 CRISPR knockout library design. Drepanos LM, Srikanth S, Kaplan EG, Shah ST, Escude Velasco B, Merzouk S, Doench JG. bioRxiv 2025.08.26.672375. https://doi.org/10.1101/2025.08.26.672375
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