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Addgene Guides & Resources

Kits

Hamdoun lab FP fusion plasmids
Integrated Genomics Plasmids
dRMCE Plasmids
biGBac Kit
cpFRET
GreenGate
AID* Kit
CRISPi Kit
SapTrap Kit
pCri System
BIOFAB
FREQ-Seq Kit
CCSB-Broad Human Kinase ORF Collection
TALE Toolbox
ZFC Modular Assembly Kit v1.0T
GoldenBraid 2.0 Kit
MXS Chaining Kit
hCRISPRi-v2, genome-wide, 10 sgRNAs/gene
Toronto KnockOut Library (TKOv1)
MoClo Pichia toolkit
Synuclein VHH Immune Library
Mobius Assembly Vector Toolkit
Ekker Lab TALEN kit
Golden Gate TALEN and TAL effector kit 2.0
Multiplex CRISPR/Cas9 Assembly System Kit
CRISPaint Gene Tagging Kit
Joung Lab REAL Assembly TALEN Kit
Platinum Gate TALEN Kit
Myristoylated Kinase Library
EasyClone-MarkerFree Vector Set
C. elegans miniMos transgenesis kit
mCRISPRa-v2, genome-wide, 10 sgRNAs/gene
MultiSite Gateway plasmid toolKit
pCut plasmid toolkit
MoClo Yeast Toolkit
MoClo Plant Parts Kit
Human SAM library in lentiSAMv2 backbone
Yamamoto Lab TALEN Accessory Pack
Endy/Shearwin pOSIP plasmid set
LIC TAL Effector Assembly Kit
CIDAR MoClo Parts Kit
Golden GATEway Cloning Kit
EcoFlex MoClo Kit
EasyClone 2.0 Yeast Toolkit
mCRISPRi-v2, genome-wide, 10 sgRNAs/gene
STAR TALE Assembly Kit
Vaccinia Virus ORF Library
Yeast Prototrophy Kit
Winslow Lab Retroviral Barcoding Library
ZFC Modular Assembly Kit v1.0B
hCRISPRa-v2, genome-wide, 10 sgRNAs/gene
Cancer Pathways ORFs Kit
FX cloning Kit
Wnt Open Source
RAS mutant clone collection
Musunuru-Cowan TALEN Kit
MoClo Plant Parts II and Infrastructure Kit
pDGE Dicot Genome Editing Kit
MoClo Tool Kit
Fire Lab C. Elegans Kit
ZFC Modular Assembly Kit v1.0S
PRESTO-Tango Kit
PUF Assembly Kit
EMMA toolkit
GoldenPiCS Kit
pGCS vector kit
RAS Pathway 2.0 Clone Collection
Hsa21 Gene Expression Set
Wolfe-Lawson ZFN modular assembly kit
FusX TALEN assembly system
Mouse GeCKO Lentiviral sgRNA Library (LentiCRISPR)
Human SAM Library in lenti sgRNA(MS2)_zeo backbone
B. subtilis Single Gene Deletion Library--Kanamycin
Target Accelerator Pan-Cancer Mutant Collection
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Unassigned1
Human GeCKO Lentiviral sgRNA Library v2 (LentiCRISPR)
Zinc Finger Consortium Expression Vector and Modular Assembly Kit
Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack
Mouse CRISPRa sgRNA library Caprano in backbone XPR_502 (P65 HSF)
Xiaole Shirley Liu lab Human CRISPR lentiviral library
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Stress Proteostasis
S. cerevisiae Advanced Gateway Destination Vectors
Minimal Antibiotic Resistance Platform (ARP)
Mouse CRISPRi sgRNA library Dolomiti in backbone XPR_050
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Membrane proteins
Target Accelerator Genetic Pathway Reference Set
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Mouse unique 1
Mouse GeCKO Lentiviral sgRNA Library (LentiGuide-Puro)
Multiple Lentiviral Expression (MuLE) system
Zinc Finger Consortium Expression Vector Kit v1.0
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF)
SapTrap-SEC kit for C. elegans genome engineering
B. subtilis CRISPRi Essential Gene Knockdown Library
Target Accelerator Lung Cancer Mutant Collection
Alexandrov Cell-Free Protein Expression Kit (PEK)
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Mouse unique 2
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Unassigned2
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Gene Expression
Mouse SAM Library in lenti sgRNA(MS2)_puro optimized backbone
Human GeCKO Lentiviral sgRNA Library v2 (lentiGuide-Puro)
Zinc Finger Consortium OPEN Accessory Reagent Set
Rinehart & Söll Phosphoprotein Synthesis Kit
Human CRISPRi sgRNA library Dolcetto in backbone XPR_050
Human Kinase Domain Constructs for Automated Bacterial Expression
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Apoptosis and cancer
Yeast GoldenBraid Cloning System and Toolkit
Human Brunello kinome pooled library, guides 1-8 in lentiCRISPRv2 backbone
Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in pLenti-sgRNA
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Drug targets, kinases, phosphatases
Human CRISPR lncRNA activation library (SAM) in lenti sgRNA(MS2)_zeo backbone
Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in lentiCRISPR v1
Sabatini/Lander Lab Activity-Optimized Genome-wide Mouse sgRNA Library in pLenti-sgRNA
Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease
Human Brunello kinome pooled library, guides 1-8 in lentiGuide-Puro backbone
Bassik lab Mouse CRISPR-Cas9 Deletion Library - Trafficking, mitochondrial, motility

Plasmids, Strains, and Pooled Libraries 1–20 of 70869

  1. pREG(R1-R3) #BGV141 Empty backbone
    Plasmid
    #48958
    Depositor
    Purpose
    Retroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Use
    Retroviral
    Tags
    None
    Expression
    Mutation
    None
  2. Plasmid
    #53245
    Depositor
    Purpose
    reporter plasmid containing 3 myc cDNA binding sites
    Insert
    pMyc3ElbLuc ( MYC Human )
    Use
    Tags
    None
    Expression
    Mutation
    To prepare plasmids with one,...
  3. Plasmid
    #53243
    Depositor
    Purpose
    reporter plasmid containing 1 myc cDNA binding sites
    Insert
    pMyc1ElbLuc ( MYC Human )
    Use
    Tags
    None
    Expression
    Mutation
    To prepare plasmids with one,...
  4. pLEG(R1-R3) #BGV149 Empty backbone
    Plasmid
    #48956
    Depositor
    Purpose
    Lentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Use
    Lentiviral
    Tags
    None
    Expression
    Mutation
    None
  5. Plasmid
    #53246
    Depositor
    Purpose
    reporter plasmid containing 4 myc cDNA binding sites
    Insert
    pMyc4ElbLuc ( MYC Human )
    Use
    Tags
    None
    Expression
    Mutation
    To prepare plasmids with one,...
  6. Bacterial strain
    #102260
    Depositor
    Purpose
    ompA, lamB double knockout strain for overproduction of outer membrane proteins
    Use
    Tags
    None
    Expression
    Mutation
    None
  7. Bacterial strain
    #42894
    Depositor
    Use
    Tags
    None
    Expression
    Mutation
    None
  8. Bacterial strain
    #102265
    Depositor
    Purpose
    ompC, ompF double knockout strain for overproduction of outer membrane proteins
    Use
    Tags
    None
    Expression
    Mutation
    None
  9. Bacterial strain
    #34928
    Depositor
    Use
    Tags
    None
    Expression
    Mutation
    None
  10. Bacterial strain
    #102258
    Depositor
    Purpose
    ompC deletion strain for overproduction of outer membrane proteins
    Use
    Tags
    None
    Expression
    Mutation
    None
  11. Bacterial strain
    #51946
    Depositor
    Purpose
    oriT-kan template
    Use
    Tags
    None
    Expression
    Mutation
    None
  12. Bacterial strain
    #69780
    Depositor
    Purpose
    MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + ΔSgrS
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Mutation
    None
  13. Bacterial strain
    #69778
    Depositor
    Purpose
    MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + Δdam + ΔOxyR
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Mutation
    None
  14. Bacterial strain
    #69773
    Depositor
    Purpose
    MG1655 + pLacO-1::T710:::YFP at GalK
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Mutation
    None
  15. Plasmid
    #53244
    Depositor
    Purpose
    reporter plasmid containing 2 myc cDNA binding sites
    Insert
    pMyc2ElbLuc ( MYC Human )
    Use
    Tags
    None
    Expression
    Mutation
    To prepare plasmids with one,...
  16. Bacterial strain
    #102262
    Depositor
    Purpose
    ompA, ompF double knockout strain for overproduction of outer membrane proteins
    Use
    Tags
    None
    Expression
    Mutation
    None
  17. Bacterial strain
    #72337
    Depositor
    Purpose
    Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA
    Use
    Tags
    None
    Expression
    Mutation
    None
  18. Bacterial strain
    #40803
    Depositor
    Use
    N/A
    Tags
    None
    Expression
    Mutation
    None
  19. Bacterial strain
    #69783
    Depositor
    Purpose
    MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + ΔOxyR
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Mutation
    None
  20. Bacterial strain
    #69771
    Depositor
    Purpose
    MC4100 + agn43 gene deleted + ΔOxyR
    Use
    Synthetic Biology
    Tags
    None
    Expression
    Mutation
    None
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