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Addgene Guides & Resources

Kits

Hamdoun lab FP fusion plasmids
dRMCE Plasmids
Integrated Genomics Plasmids
BIOFAB
FREQ-Seq Kit
biGBac Kit
cpFRET
SapTrap Kit
pCri System
GreenGate
MoClo Tool Kit
Fire Lab C. Elegans Kit
ZFC Modular Assembly Kit v1.0S
PUF Assembly Kit
PRESTO-Tango Kit
RAS Pathway 2.0 Clone Collection
pGCS vector kit
Wolfe-Lawson ZFN modular assembly kit
FusX TALEN assembly system
CCSB-Broad Human Kinase ORF Collection
TALE Toolbox
ZFC Modular Assembly Kit v1.0T
GoldenBraid 2.0 Kit
hCRISPRi-v2, genome-wide, 10 sgRNAs/gene
MXS Chaining Kit
Toronto KnockOut Library (TKOv1)
MoClo Pichia toolkit
Synuclein VHH Immune Library
Ekker Lab TALEN kit
ZFC Modular Assembly Kit v1.0B
Cancer Pathways ORFs Kit
hCRISPRa-v2, genome-wide, 10 sgRNAs/gene
Wnt Open Source
RAS mutant clone collection
FX cloning Kit
Musunuru-Cowan TALEN Kit
pDGE Dicot Genome Editing Kit
Human SAM library in lentiSAMv2 backbone
Yamamoto Lab TALEN Accessory Pack
MoClo Yeast Toolkit
MoClo Plant Parts Kit
LIC TAL Effector Assembly Kit
CIDAR MoClo Parts Kit
Golden GATEway Cloning Kit
Endy/Shearwin pOSIP plasmid set
EasyClone 2.0 Yeast Toolkit
Vaccinia Virus ORF Library
EcoFlex MoClo Kit
mCRISPRi-v2, genome-wide, 10 sgRNAs/gene
Yeast Prototrophy Kit
STAR TALE Assembly Kit
Winslow Lab Retroviral Barcoding Library
Golden Gate TALEN and TAL effector kit 2.0
Multiplex CRISPR/Cas9 Assembly System Kit
Joung Lab REAL Assembly TALEN Kit
CRISPaint Gene Tagging Kit
Platinum Gate TALEN Kit
Myristoylated Kinase Library
CRISPRainbow Kit for Multiplex Labeling
C. elegans miniMos transgenesis kit
mCRISPRa-v2, genome-wide, 10 sgRNAs/gene
pCut plasmid toolkit
EasyClone-MarkerFree Vector Set
MultiSite Gateway plasmid toolKit
Human GeCKO Lentiviral sgRNA Library v2 (lentiGuide-Puro)
Mouse SAM Library in lenti sgRNA(MS2)_puro optimized backbone
Zinc Finger Consortium OPEN Accessory Reagent Set
Rinehart & Söll Phosphoprotein Synthesis Kit
Human Kinase Domain Constructs for Automated Bacterial Expression
Mouse GeCKO Lentiviral sgRNA Library (LentiCRISPR)
Human SAM Library in lenti sgRNA(MS2)_zeo backbone
B. subtilis Single Gene Deletion Library--Kanamycin
Target Accelerator Pan-Cancer Mutant Collection
Mouse GeCKO Lentiviral sgRNA Library (LentiGuide-Puro)
Multiple Lentiviral Expression (MuLE) system
Zinc Finger Consortium Expression Vector Kit v1.0
SapTrap-SEC kit for C. elegans genome engineering
B. subtilis CRISPRi Essential Gene Knockdown Library
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF)
Target Accelerator Lung Cancer Mutant Collection
Alexandrov Cell-Free Protein Expression Kit (PEK)
S. cerevisiae Advanced Gateway Destination Vectors
Target Accelerator Genetic Pathway Reference Set
Human GeCKO Lentiviral sgRNA Library v2 (LentiCRISPR)
Zinc Finger Consortium Expression Vector and Modular Assembly Kit
Human SAM library in lenti sgRNA(MS2)_puro backbone
Multiplex CRISPR dCas9/FokI-dCas9 Accessory Pack
Human Brunello kinome pooled library, guides 1-8 in lentiGuide-Puro backbone
Tol2 Gateway compatible toolbox for the study of the nervous system and neurodegenerative disease
Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in pLenti-sgRNA
Human Brunello kinome pooled library, guides 1-8 in lentiCRISPRv2 backbone
Sabatini/Lander Lab Activity-Optimized Genome-wide Mouse sgRNA Library in pLenti-sgRNA
Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in lentiCRISPR v1
Human CRISPR lncRNA activation library (SAM) in lenti sgRNA(MS2)_zeo backbone

Plasmids

  1. pREG(R1-R3) #BGV141 (Plasmid #48958) Empty backbone
    Purpose Retroviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Tags: None
    Expression:
    Mutation: None
    Use: Retroviral
    Deposited by: David Dankort
  2. pMyc4ElbLuc. (Plasmid #53246)
    Purpose reporter plasmid containing 4 myc cDNA binding sites
    Insert: pMyc4ElbLuc ( MYC Human )
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  3. pLEG(R1-R3) #BGV149 (Plasmid #48956) Empty backbone
    Purpose Lentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).
    Tags: None
    Expression:
    Mutation: None
    Use: Lentiviral
    Deposited by: David Dankort
  4. pMyc1ElbLuc, (Plasmid #53243)
    Purpose reporter plasmid containing 1 myc cDNA binding sites
    Insert: pMyc1ElbLuc ( MYC Human )
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  5. pMyc2ElbLuc, (Plasmid #53244)
    Purpose reporter plasmid containing 2 myc cDNA binding sites
    Insert: pMyc2ElbLuc ( MYC Human )
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  6. pMyc3ElbLuc (Plasmid #53245)
    Purpose reporter plasmid containing 3 myc cDNA binding sites
    Insert: pMyc3ElbLuc ( MYC Human )
    Tags: None
    Expression:
    Mutation: To prepare plasmids with one,...
    Use:
    Deposited by: Roger Davis
  7. BL21∆serB (Bacterial strain #34929)
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Jesse Rinehart
  8. MG1655 ΔendA ΔrecA (Bacterial strain #37853)
    Tags: None
    Expression:
    Mutation: None
    Use: E. coli bacterial strain
    Deposited by: Kristala Prather
  9. HL 6777 (Bacterial strain #69781)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔSgrS
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  10. HL 3500 (Bacterial strain #69774)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  11. BL21ΔBF (Bacterial strain #102264)
    Purpose lamB, ompF double knockout strain for overproduction of outer membrane proteins
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Jack Leo
  12. BL21ΔB (Bacterial strain #102257)
    Purpose lamB deletion strain for overproduction of outer membrane proteins
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Jack Leo
  13. nuc4- (Bacterial strain #40803)
    Tags: None
    Expression:
    Mutation: None
    Use: N/A
    Deposited by: George Church
  14. CY007 (Bacterial strain #72337)
    Purpose Bacterial strain: E. coli BW25113 ΔlacI ΔaraC ΔsdiA
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Matthew Bennett
  15. HL 6779 (Bacterial strain #69783)
    Purpose MG1655 + pLacO-1::T710:::YFP at GalK + ΔLacI + ΔOxyR
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  16. HL372 (Bacterial strain #69771)
    Purpose MC4100 + agn43 gene deleted + ΔOxyR
    Tags: None
    Expression:
    Mutation: None
    Use: Synthetic Biology
    Deposited by: Han Lim
  17. BL21ΔAF (Bacterial strain #102262)
    Purpose ompA, ompF double knockout strain for overproduction of outer membrane proteins
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Jack Leo
  18. MG1655 ΔendA ΔrecA (DE3) (Bacterial strain #37854)
    Tags: None
    Expression:
    Mutation: None
    Use: E. coli bacterial strain
    Deposited by: Kristala Prather
  19. EcNR2.xseA- (Bacterial strain #40802)
    Tags: N/A
    Expression:
    Mutation: None
    Use: N/A
    Deposited by: George Church
  20. rEc 27.H.Sc (Bacterial strain #51948)
    Purpose specR template
    Tags: None
    Expression:
    Mutation: None
    Use:
    Deposited by: Farren Isaacs
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