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Addgene
Showing: 1 - 40 of 3777 results
  1. EED

    Plasmid
    #25311
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    EED-6 (EED Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 25, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
  2. 5TTW (EED)

    Plasmid
    #101254
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    EED (EED Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 30, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. EED

    Plasmid
    #25311
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    EED-6 (EED Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 25, 2011
    Availability
    Industry, Academic Institutions, and Nonprofits
  4. Biomolecular FeedBack (FB)

    Plasmid
    #109391
    Purpose
    This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.
    Depositor
    Insert
    pJ23100mut-dCas9-T- phtpG1-sgRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
  5. pAS-8 (seed1-8)

    Plasmid
    #40843
    Purpose
    Target RNAs used to determine complementarity requirements for in vitro tailing and degradation
    Depositor
    Insert
    let-7–seed1-8
    Use
    Mirna reporter
    Tags
    EGFP
    Expression
    Insect
    Mutation
    Promoter
    Actin5C
    Available since
    Sept. 4, 2013
    Availability
    Academic Institutions and Nonprofits only
  6. pFastBacDual with LFn + Needle

    Plasmid
    #84868
    Purpose
    Baculovirus Expression of LFn-Needle fusion. The LFn domain acts as a signal sequence that targets proteins for cytosolic translocation via the co-administered protective antigen (PA) channel protein
    Depositor
    Inserts
    LFn
    Needle
    Use
    Baculovirus
    Tags
    6xHis
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    Nov. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
  7. pTRIPZ (M)-YFP-Eed

    Plasmid
    #82512
    Purpose
    Lentivirus vector. Expresses YFP-Eed fusion proteins in mammalian cells.
    Depositor
    Insert
    embryonic ectoderm development
    Use
    Lentiviral
    Tags
    HaloTag
    Expression
    Mammalian
    Mutation
    insert contains AA39-441 of EeD
    Promoter
    cmv
    Available since
    Dec. 1, 2016
    Availability
    Academic Institutions and Nonprofits only
  8. mutpRL-TK 1x perfect to seed siRNA

    Plasmid
    #40766
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Insert
    CXCR4 small RNA perfect to seed target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
  9. mutpRL-TK 1x perfect to seed plus 13-16 siRNA

    Plasmid
    #40767
    Purpose
    To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA
    Depositor
    Insert
    CXCR4 small RNA perfect to seed plus 13-16 target site
    Use
    Luciferase
    Tags
    luciferase
    Expression
    Mutation
    mutated from TAG to CTC at nt 387–389 of the Reni…
    Promoter
    TK
    Available since
    Sept. 26, 2013
    Availability
    Academic Institutions and Nonprofits only
  10. 5TTW (EED)

    Plasmid
    #101254
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    EED (EED Human)
    Use
    Tags
    6xHis-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 30, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
  11. pGWG_RUBYseed

    Plasmid
    #225989
    Purpose
    To clone any gene of interest using gateway recombination cloning and the vector expresses seed-specific RUBY
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Oct. 16, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
  12. Biomolecular FeedBack (FB)

    Plasmid
    #109391
    Purpose
    This construct encodes for two units. The first contains a constitutively expressed dCas9 protein. The second contains an sgRNA cassette under the control of the burden-responsive htpG1 promoter.
    Depositor
    Insert
    pJ23100mut-dCas9-T- phtpG1-sgRNA
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 16, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 40 of 3777 results