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pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

Plasmid

#35152

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5, pTrc

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT(CO)-T1-MBIS(CO, ispA)

Plasmid

#35152

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-pTrc-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5, pTrc

Available Since

May 16, 2012

Availability

Academic Institutions and Nonprofits only

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Human Mevalonate Kinase (MK)

Plasmid

#52429

Purpose

bacterial expression of human Mevalonate Kinase (MK)

Depositor

Henry Miziorko

Article

Potter et al J Biol Chem. 1997 Oct 10;272(41):25449-5

Insert

Human Mevalonate Kinase (MVK Human)

Expression

Bacterial

Mutation

see comments

Promoter

T7

Available Since

April 10, 2014

Availability

Academic Institutions and Nonprofits only
Human Mevalonate Kinase (MK)

Plasmid

#52429

Purpose

bacterial expression of human Mevalonate Kinase (MK)

Depositor

Henry Miziorko

Article

Potter et al J Biol Chem. 1997 Oct 10;272(41):25449-5

Insert

Human Mevalonate Kinase (MVK Human)

Expression

Bacterial

Mutation

see comments

Promoter

T7

Available Since

April 10, 2014

Availability

Academic Institutions and Nonprofits only
pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbA5c-MevT(CO)-MBIS(CO, ispA)

Plasmid

#35151

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Mutation

R364S in Mevalonate Kinase

Promoter

LacUV5,

Available Since

July 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
MDKA-c014

Plasmid

#175464

Purpose

Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143. Fused to IgG Fc.

Depositor

Opher Gileadi

Insert

MDK (MDK Human)

Tags

HRV 3C-Fc-His6 and PTPRS (Gg) SP

Expression

Mammalian

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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MDKA-c009

Plasmid

#175463

Purpose

Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143

Depositor

Opher Gileadi

Insert

MDK (MDK Human)

Tags

His6 and PTPRS (Gg) SP

Expression

Mammalian

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MDKA-c014

Plasmid

#175464

Purpose

Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143. Fused to IgG Fc.

Depositor

Opher Gileadi

Insert

MDK (MDK Human)

Tags

HRV 3C-Fc-His6 and PTPRS (Gg) SP

Expression

Mammalian

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
MDKA-c009

Plasmid

#175463

Purpose

Protein expression/secretion in mammalian cells. Entire CDS with ectopic signal peptide, V21-D143

Depositor

Opher Gileadi

Insert

MDK (MDK Human)

Tags

His6 and PTPRS (Gg) SP

Expression

Mammalian

Available Since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105210

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLN193 (SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105210

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 3 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv3 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

March 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLN520 (SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105207

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 1 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLN520 (SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105207

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 1 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv1 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLN189 (SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105209

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 2 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLN189 (SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe))

Plasmid

#105209

Purpose

Module 1 - SSX1 promoter drives self-inhibiting mKate2 expression (Version 2 intron - see PMID: 29056342 for detailed information)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

SSX1p-[mK-Ex1]-[miR1-Mv2 intron]-[mK-Ex2]-BS(Pe)

Use

Synthetic Biology

Expression

Mammalian

Available Since

Nov. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
JBEI-3716

Plasmid

#100169

Purpose

Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

Depositor

Jay Keasling

Article

Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

Insert

codon-optimized MK

Expression

Bacterial

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
JBEI-3716

Plasmid

#100169

Purpose

Used to improve production from engineered biosynthetic pathways include optimizing codon usage, enhancing production of rate-limiting enzymes, and eliminating the accumulation of toxic intermediates or byproducts to improve cell growth

Depositor

Jay Keasling

Article

Anthony et al Metab Eng. 2009 Jan;11(1):13-9. doi: 10.

Insert

codon-optimized MK

Expression

Bacterial

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
JBEI-12050

Plasmid

#84982

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

MevTo-T21-MK-aphA

Expression

Bacterial

Promoter

LacUV5

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
JBEI-12050

Plasmid

#84982

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

MevTo-T21-MK-aphA

Expression

Bacterial

Promoter

LacUV5

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge