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Plasmid#205800PurposeExpress mEGFP-tagged fusion protein, CUX1_NUTM1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only
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pNUT1
Plasmid#175746PurposeSingle-component photo-inducible (LOV2-based) nucleolus-targeting tool 1DepositorInsertmCherry-LOV2-NoLS (HIV Rev transactivating protein)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNUT3
Plasmid#175744PurposeSingle-component photo-inducible (CRY2-based) nucleolus-targeting tool 3DepositorInsertmCherry-CRY2PHR-NoLS (human nuclear factor-κB inducing kinase)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
CL20_mEGFP_BRD9_NUTM1
Plasmid#205783PurposeExpress mEGFP-tagged fusion protein, BRD9_NUTM1 from patient-derived sequenceDepositorUseTagsmEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNUT2
Plasmid#175745PurposeSingle-component photo-inducible (LOV2-based) nucleolus-targeting tool 2DepositorInsertmCherry-LOV2-NoLS (human nuclear factor-κB inducing kinase)
UseTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
Doughnut (DNT)
Plasmid#17580DepositorInsertdnt (dnt Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His hTFNG
Plasmid#67240PurposeExpresses N-His tagged nonglycosylated human serum transferrin in mammalian cellsDepositorInserthuman serum transferrin (TF Human)
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationAsn413 Asp, Asn611AspPromoterSV40Available sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His mICA NG
Plasmid#70162PurposeExpresses N-His tagged nonglycosylated mouse inhibitor of carbonic anhydraseDepositorInsertmouse inhibitor of carbonic anhydrase
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationAsn470Asp and Asn645AspPromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pnCS_mEGFP-DSep2_Peanut-Strep
Plasmid#174496Purposebacterial co-expression of Drosophila Sep2 fused to monomeric EGFP and of Drosophila PeanutDepositorUseTagsStrep and mEGFPExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pnCS_DSep2_Peanut-TEV-Strep
Plasmid#174495Purposebacterial co-expression of Drosophila Sep2 and of Drosophila PeanutDepositorUseTagsTEV-StrepExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
HaloTag-bActinCDS-bActinUTR-MS2V5
Plasmid#102718PurposeHalo-Actin reporter for detection of both mRNA and proteinDepositorInsertHaloTag-bActinCDS-bActin3'UTR-MS2V5
UseLentiviralTagsHaloTag and MS2V5ExpressionMutationPromoterUbCAvailable sinceNov. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His K206E hTFNG
Plasmid#70130PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobeDepositorInsertmutated human serum transferrin (TF Human)
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationchanged Lys206 to a glutamic acid, Asn413Asp, Asn…PromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNUTN6His Y426F/Y517F hTFNG
Plasmid#70084PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the C-lobeDepositorInsertmutated human serum transferrin (TF Human)
UseTagsHexa His tag and N-terminal signal peptide, 4 aa …ExpressionMammalianMutationAsn413 Asp, Asn611Asp, Ty426Phe, Tyr517PhePromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-NUT
Plasmid#204693PurposeThis plasmid allows for inducible expression of full-length NUT tagged with mNeonGreen, in mammalian cells.DepositorInsertNUT (NUTM1 Human)
UseTagsmNeonGreenExpressionMammalianMutationn/aPromoterCMVAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT
Plasmid#204694PurposeThis plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdirect fusion of BRD4 and NUTPromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 frt/to N-BioTAP-C-BRD4-NUT
Plasmid#171630PurposeExpresses BioTAP-tagged BRD4-NUT in mammalian cells. Can be inserted into a FRT site by co-transfection with a plasmid encoding Flp recombinaase.DepositorUseTagsBioTAPExpressionMammalianMutationPromoterAvailable sinceAug. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His K206E/K534A hTFNG
Plasmid#70133PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobeDepositorInsertN6His K206e/K534A hTFNG (TF Human)
UseTagsN-terminal signal peptide, 4 aa link, 6 His, Fact…ExpressionMammalianMutationchanged Lys206 to a glutamic acid and Lys534 to a…PromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNUT N6His Y95F/Y188F hTFNG
Plasmid#69601PurposeExpresses N-His tagged nonglycosylated human serum transferrin unable to bind iron in the N-lobeDepositorInsertmutated human serum transferrin (TF Human)
UseTagsHexa His tag and N-terminal signal peptide, 4 aa …ExpressionMammalianMutationAsn413 Asp, Asn611Asp, Tyr95Phe, Tyr188PhePromoterSV40Available sinceNov. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)
Plasmid#204696PurposeThis plasmid allows for inducible expression of BRD4-NUT(deltaMIN) mutant fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdeletion of the amino acids 355-505 of NUTPromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(MIN)
Plasmid#204695PurposeThis plasmid allows for inducible expression of BRD4-NUT(MIN) mutant fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdeletion of majority of NUT, retaining only amino…PromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only