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pUNC2001

Plasmid

#67280

Purpose

Expresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcw

Depositor

Aziz Sancar

Article

Worthington et al J Biol Chem. 2003 Oct 3;278(40):39143-54

Insert

Vibrio cholera photolyase

Tags

maltose binding protein

Expression

Bacterial

Mutation

none known

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
pUNC2001

Plasmid

#67280

Purpose

Expresses MBP-Vibrio cholera photolyase in E. coli, vector pMalcw

Depositor

Aziz Sancar

Article

Worthington et al J Biol Chem. 2003 Oct 3;278(40):39143-54

Insert

Vibrio cholera photolyase

Tags

maltose binding protein

Expression

Bacterial

Mutation

none known

Available Since

July 23, 2015

Availability

Academic Institutions and Nonprofits only
pWSK29-wzzO139

Plasmid

#172970

Purpose

pWSK29 with Vibrio cholerae wzzO139 gene

Depositor

Renato Morona

Article

Murray et al J Bacteriol. 2006 Apr;188(7):2735-9. doi

Insert

wzz gene from Vibrio cholerae O139

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pPM1003

Plasmid

#167550

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Ogawa serotype

Use

Cosmid

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pPM1001

Plasmid

#167548

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Inaba serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Inaba serotype

Use

Cosmid

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Vibrio_vulnificus

Plasmid

#224405

Purpose

For expression of the type I CBASS system encoded by Vibrio vulnificus

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Endonuclease_Phospholipase_VvCdnG_AGS-C

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pBAD02_CBASS_Vibrio_campbellii

Plasmid

#224404

Purpose

For expression of the type I CBASS system encoded by Vibrio campbellii

Depositor

Philip Kranzusch

Article

Richmond-Buccola et al Cell Host Microbe. 2024 Jul 10;32(7):107

Insert

Endonuclease_VcCdnG_AGS-C

Expression

Bacterial

Available Since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pMarC9-R6k

Plasmid

#89477

Purpose

transposon plasmid, compatible with Tn-Seq

Depositor

George Church

Article

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pMarC9-R6k

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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Gal tr-lux

Plasmid

#1330

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

Bacterial luciferase-V.h.CTP5

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
Gal ts-lux

Plasmid

#1329

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

bacterial luciferase-V.h.MAV

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
Gal tr-lux

Plasmid

#1330

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

Bacterial luciferase-V.h.CTP5

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
Gal ts-lux

Plasmid

#1329

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

bacterial luciferase-V.h.MAV

Expression

Yeast

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pMarC9-R6k

Plasmid

#89477

Purpose

transposon plasmid, compatible with Tn-Seq

Depositor

George Church

Article

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pMarC9-R6k

Available Since

Oct. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pRSF-pLtetO-gfp

Plasmid

#89476

Purpose

Broad-host plasmid expressing gfp under pLtetO promoter

Depositor

George Church

Article

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pLtetO-GFPmut3b

Promoter

pLtetO

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCM18

Plasmid

#78565

Purpose

60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmid

Depositor

James Kaper

Article

Morin et al FEMS Immunol Med Microbiol. 2009 Oct;57(

Type

Empty backbone

Tags

luxCDABE

Expression

Bacterial

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pRSF-pLtetO-gfp

Plasmid

#89476

Purpose

Broad-host plasmid expressing gfp under pLtetO promoter

Depositor

George Church

Article

Lee et al Nat Microbiol. 2019 Apr 8. pii: 10.1038/

Insert

pLtetO-GFPmut3b

Promoter

pLtetO

Available Since

Oct. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCVD518

Plasmid

#66158

Purpose

Six copies of the 415 bp PstI fragment of pCVD512, carrying the tdh1 gene probe from Vibrio parahaemolyticus WP1, for generation of probe

Depositor

James Kaper

Article

Nishibuchi et al Infect Immun. 1985 Sep;49(3):481-6.

Insert

tdh1

Use

Cloning vector

Promoter

Unknown

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only
pST_116_LVL2 cam

Plasmid

#179332

Purpose

NT-CRISPR plasmid for a single gRNA.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pST_140_LVL2 cam

Plasmid

#179334

Purpose

NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Mutation

Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCM18

Plasmid

#78565

Purpose

60-copy, promoterless, oriE1(ColE1) luxCDABE, aph, hok-sok, par, parA, rrnB Stabilized luciferase-expressing plasmid

Depositor

James Kaper

Article

Morin et al FEMS Immunol Med Microbiol. 2009 Oct;57(

Type

Empty backbone

Tags

luxCDABE

Expression

Bacterial

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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