We narrowed to 6,856 results for: lenti crispr cas9 plasmids

1. lentiCas9-Blast (Lentiviral Prep)

   Viral Prep

   #52962-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Promoter

   EFS-NS

   Tags

   None

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

2. Human sgRNA library Brunello in lentiCRISPRv2 (Lentiviral Prep)

   Viral Prep

   #73179-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiCRISPRv2 (#73179). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2 plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. This backbone contains SpCas9 and unique gRNAs, and can be used to make edits across 19,114 genes in the human genome. In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiCRISPRv2.

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Promoter

   Tags

   None

   Available since

   June 14, 2017

   Availability

   Academic Institutions and Nonprofits only

3. dCAS9-VP64_GFP (Concentrated Lentiviral Prep)

   Viral Prep

   #61422-LVC

   Purpose

   Ready-to-use Concentrated Lentiviral Prep particles produced from dCAS9-VP64_GFP (#61422). In addition to the viral particles, you will also receive purified dCAS9-VP64_GFP plasmid DNA.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Promoter

   EF1a

   Tags

   None

   Available since

   Aug. 24, 2016

   Availability

   Academic Institutions and Nonprofits only

4. Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A (Lentiviral Prep)

   Viral Prep

   #92385-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A (#92385). In addition to the viral particles, you will also receive purified Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set A plasmid DNA.

   Depositor

   David Root, John Doench

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Promoter

   Tags

   None

   Available since

   Sept. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

5. Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (Lentiviral Prep)

   Viral Prep

   #92379-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A (#92379). In addition to the viral particles, you will also receive purified Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set A plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR activation screening in human cells. Contains 56,762 sgRNAs, targeting 18,885 genes. Concentrated lentiviral particles carrying set A of the Calabrese human CRISPRa sgRNA activation library in backbone XPR_502 (P65 HSF) .

   Depositor

   David Root, John Doench

   Article

   Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

   Promoter

   Tags

   None

   Available since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

6. Human sgRNA library Brunello in lentiGuide-Puro (Lentiviral Prep)

   Viral Prep

   #73178-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from Human sgRNA library Brunello in lentiGuide-Puro (#73178). In addition to the viral particles, you will also receive purified Human sgRNA library Brunello in lentiGuide-Puro plasmid DNA. Ready-to-use lentiviral pooled library for CRISPR screening in human cells. Use on cells that are stably expressing Cas9 to make edits across 19,114 genes in the human genome.

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Promoter

   Tags

   None

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

7. Mouse sgRNA library Brie in lentiGuide-Puro (Lentiviral Prep)

   Viral Prep

   #73633-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from Mouse sgRNA library Brie in lentiGuide-Puro (#73633). In addition to the viral particles, you will also receive purified Mouse sgRNA library Brie in lentiGuide-Puro plasmid DNA. <p>&lt;p&gt;Ready-to-use lentiviral pooled library for CRISPR screening in mouse cells. Use on cells that are stably expressing Cas9 to make edits across 19,674 genes in the mouse genome.&lt;/p&gt;</p>

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Promoter

   Tags

   None

   Available since

   Aug. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

8. lenti dCAS-VP64_Blast (Lentiviral Prep)

   Viral Prep

   #61425-LV

   Purpose

   Ready-to-use Lentiviral Prep particles produced from lenti dCAS-VP64_Blast (#61425). In addition to the viral particles, you will also receive purified lenti dCAS-VP64_Blast plasmid DNA.

   Depositor

   Feng Zhang

   Article

   Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

   Promoter

   EF1a

   Tags

   None

   Available since

   July 14, 2016

   Availability

   Academic Institutions and Nonprofits only

9. lentiCRISPR v2-dCas9

   Plasmid

   #112233

   Purpose

   Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

   Depositor

   Thomas Gilmore

   Article

   Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

   Inserts

   dCas9
   Porumycin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Changed: Aspartic acid 10 to Glycine, Histidine 8…

   Promoter

   EFS-NS

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR v2-dCas9

    Plasmid

    #112233

    Purpose

    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2

    Depositor

    Thomas Gilmore

    Article

    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do

    Inserts

    dCas9
    Porumycin resistance
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Changed: Aspartic acid 10 to Glycine, Histidine 8…

    Promoter

    EFS-NS

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti_dCas9-2xAM

    Plasmid

    #92220

    Purpose

    Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Inserts

    Sp-dCas9-2xAM tag
    gRNA to be inserted into Bbs I sites
    

    Use

    CRISPR and Lentiviral

    Tags

    2xAM tag

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    CBh and U6

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti.Cas9.BFP.sgRNA.parental

    Plasmid

    #196715

    Purpose

    Sortable WT-SpCas9 expression and guide cassette (1-vector system).

    Depositor

    Bernhard Schmierer

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti.Cas9.BFP.Blast

    Plasmid

    #196714

    Purpose

    WT SpCas9 with BSD-TagBFP for selection and sorting

    Depositor

    Bernhard Schmierer

    Insert

    Cas9-T2A-BSD-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti_dCas9-2xAM

    Plasmid

    #92220

    Purpose

    Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Inserts

    Sp-dCas9-2xAM tag
    gRNA to be inserted into Bbs I sites
    

    Use

    CRISPR and Lentiviral

    Tags

    2xAM tag

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    CBh and U6

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_dCas9-2xAM_hIRF-1

    Plasmid

    #92221

    Purpose

    Lentiviral plasmid expressing dCas9-2xAM and gRNA for human IRF-1 promoter

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Inserts

    Sp-dCas9-2xAM tag
    gRNA targeting human IRF-1 promoter
    

    Use

    CRISPR and Lentiviral

    Tags

    2xAM tag

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    CBh and U6

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiCRISPRv2_PROX1_1+3

    Plasmid

    #121176

    Purpose

    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

    Depositor

    Timo Otonkoski

    Insert

    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiCRISPRv2_PROX1_1+4

    Plasmid

    #121177

    Purpose

    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.

    Depositor

    Timo Otonkoski

    Insert

    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. LentiV_Cas9_puro

    Plasmid

    #108100

    Purpose

    Lentiviral expression plasmid of spCas9 with puromycin resistance gene

    Depositor

    Christopher Vakoc

    Article

    Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

    Insert

    Cas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EFS promoter

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. lenti-dCas9-VP64-BFP

    Plasmid

    #196711

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-VP64-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiCRISPR v2-Blast

    Plasmid

    #83480

    Purpose

    Mammalian expression of Cas9 and sgRNA scaffold

    Depositor

    Mohan Babu

    Insert

    Blasticidin S deaminase
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Replaced puromycin N-acetyltransferase on the ori…

    Promoter

    EF-1α

    Available since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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