Addgene

We narrowed to 3,848 results for: PREP

Showing: 271 - 300 of 3848 results

Results per page:

Sort results by:

pLD3 translation factors

Plasmid

#117762

Purpose

BioBrick encoding cistrons 22-30 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 22-30

Use

Escherichia coli

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLD2 translation factors

Plasmid

#117761

Purpose

BioBrick encoding cistrons 14-21 in pET for preparation of PURE 3.0 translation system from E. coli

Depositor

Anthony Forster

Article

Shepherd et al Nucleic Acids Res. 2017 Oct 13;45(18):10

Insert

Translation factor cistrons 14-21

Use

Escherichia coli

Tags

Expression

Mutation

Promoter

Available since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pVP2A-PIF6

Plasmid

#73369

Purpose

Expresses Adeno-associated virus VP2 with PIF6 insert.

Depositor

Junghae Suh

Article

Gomez et al ACS Nano. 2015 Nov 30.

Insert

PIF6 (PIL2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-M2-TLR2

Plasmid

#12291

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I and H202Y; signal peptide (aa1-23) replaced …

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-FW/AA

Plasmid

#12293

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y, F774A and W775A; signal peptide (aa1…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pISH-Vmn2r65

Plasmid

#108083

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r65 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r65 (Vmn2r65 Mouse)

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Vmn2r76-VR2

Plasmid

#108085

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r76 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r76 (Vmn2r76 Mouse)

Use

In vitro transcription

Tags

Expression

Mutation

Promoter

Available since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Vmn2r118

Plasmid

#108086

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r118 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r118 (Vmn2r118 Mouse)

Use

In vitro transcription

Tags

Expression

Mutation

changed T to C at nt 830 of NM_001104582.1

Promoter

Available since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pISH-Vmn2r120-TR

Plasmid

#108087

Purpose

Preparation of RNA probe for ISH of mouse Vmn2r120 gene

Depositor

Peter Mombaerts

Article

Akiyoshi et al Eur J Neurosci. 2018 Apr;47(7):887-900.

Insert

Vmn2r120 (Vmn2r120 Mouse)

Use

In vitro transcription

Tags

Expression

Mutation

changed A to G at nt 1196 of NM_001104591.1

Promoter

Available since

May 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIP-Laforin Myc 3CS (C250S,C266S,C278S)

Plasmid

#84661

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc 3CS_new (C266S, C278S, C329S)

Plasmid

#84662

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pET30a-Tn5

Plasmid

#127917

Purpose

Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

Tn5

Use

Tags

Mxe intein - Chitin-binding domain

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
WT (REP10)

Plasmid

#21368

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-M2-TLR2-V660N

Plasmid

#12292

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

M82I, H202Y and V660N; signal peptide (aa1-23) re…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
pCMV-M2-TLR2-P681H

Plasmid

#12290

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

TLR2 (Tlr2 Mouse)

Use

Tags

FLAG (FLAG-M2)

Expression

Mammalian

Mutation

A74V, M82I, H202Y and P681H; signal peptide (aa1-…

Promoter

Available since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only
LB1365 (Bcl-2 promoter with MHS34)

Plasmid

#15389

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with MHS34

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
LB1362 (Bcl-2 promoter with MHS3)

Plasmid

#15390

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with MHS3

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA3.1(+)-CMV-bArrestin2-TEV

Plasmid

#107245

Purpose

Vector for preparation of stable cell lines expressing Barrestin2-TEV fusion protein in lieu of the HTLA cell line.

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

ARRB2

Use

Tags

TEV(NIA), tobacco etch virus NIA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
LB1353 (Bcl-2 promoter, no IgH enh)

Plasmid

#15393

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 with full promoter and no IgH enhancers MHS…

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pMyc4ElbLuc.

Plasmid

#53246

Purpose

reporter plasmid containing 4 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc4ElbLuc (MYC Human)

Use

Tags

Expression

Mutation

To prepare plasmids with one, two, three, and fou…

Promoter

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pMyc3ElbLuc

Plasmid

#53245

Purpose

reporter plasmid containing 3 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc3ElbLuc (MYC Human)

Use

Tags

Expression

Mutation

To prepare plasmids with one, two, three, and fou…

Promoter

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pBS GDF10

Plasmid

#19186

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

Growth differentiation factor 10 precursor (GDF10 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only
pMyc1ElbLuc,

Plasmid

#53243

Purpose

reporter plasmid containing 1 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc1ElbLuc (MYC Human)

Use

Tags

Expression

Mutation

To prepare plasmids with one, two, three, and fou…

Promoter

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
LB1366 (Bcl-2 promoter with MHS1234)

Plasmid

#15388

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with IgH full enhancer MHS1234

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
LB1376 (Bcl-2 P1 promoter with MHS1234)

Plasmid

#15387

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 P1 promoter with IgH full enhancer MHS1234

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
LB1377 (Bcl-2 P2 promoter with MHS1234)

Plasmid

#15386

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 P2 promoter with IgH full enhancer MHS1234

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
LB1361 (Bcl-2 promoter with MHS12)

Plasmid

#15391

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Bcl-2-Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with MHS12

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pMyc2ElbLuc,

Plasmid

#53244

Purpose

reporter plasmid containing 2 myc cDNA binding sites

Depositor

Roger Davis

Article

Gupta et al Proc Natl Acad Sci U S A. 1993 Apr 15;90

Insert

pMyc2ElbLuc (MYC Human)

Use

Tags

Expression

Mutation

To prepare plasmids with one, two, three, and fou…

Promoter

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
LB1360 (Bcl-2 promoter with MHS4)

Plasmid

#15392

Depositor

Linda Boxer

Article

Duan et al Oncogene. 2007 Apr 19. 26(18):2635-41.

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial and Mammalian

Mutation

Bcl-2 full promoter with IgH enhancers MHS4

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pET30a-HS-Tn5

Plasmid

#127916

Purpose

Prokaryotic expression of Hyperstable Tn5 (HS-Tn5) for Illumina library preperation. Developed for the large scale ChIP-Seq and ATAC-Seq experiments for the fruitENCODE and Maize Cistrome projects.

Depositor

Silin Zhong

Article

Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:

Insert

Hyperstable Tn5

Use

Tags

E coli elongation factor Ts and Mxe intein - Chit…

Expression

Bacterial

Mutation

E54K, L372P

Promoter

T7

Available since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge