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We narrowed to 9,696 results for: 221

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Showing: 451 - 500 of 9696 results

  1. 1203AB-CMV-Cas7.1-H43A-mutant

    Plasmid
    #221541
    Purpose
    Effector expresses Cas7.1 (truncated from DiCas7-11), H to A mutation at amino acid 43
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221541-plasmid-map-sequence-id-437821

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  2. 1203AC-CMV-Cas7.1-Y55A-mutant

    Plasmid
    #221542
    Purpose
    Effector expresses Cas7.1 domain (truncated from DiCas7-11), Y to A mutation at amino acid 55
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221542-plasmid-map-sequence-id-437820

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  3. 1203AD-CMV-Cas7.1-N152A-mutant

    Plasmid
    #221543
    Purpose
    ffector expresses Cas7.1 domain (truncated from DiCas7-11), N to A mutation at amino acid 152
    Depositor
    Omar Akbari
    Insert
    Truncated DiCas7-11
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    221543-plasmid-map-sequence-id-437816

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  4. AAV-hSyn-PiGM-Iq(535/81, eGFP)

    Plasmid
    #221612
    Purpose
    AAV encoding PiGM-Iq with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).
    Depositor
    John Lin
    Insert
    PiGM-Iq (535/81,eGFP)
    Use
    AAV
    Mutation
    RGS2 1-53 truncation
    Promoter
    Human Synapsin
    Available Since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    221612-plasmid-map-sequence-id-461237

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  5. AAV-hSyn-PiGM-Ii (149, 535/81, eGFP)

    Plasmid
    #221610
    Purpose
    AAV encoding the 149 amino acid version PiGM-Ii with minimal RGS10 domain, CRY2 (535 amino acid version), CIB1 (81 amino acid version).
    Depositor
    John Lin
    Insert
    PiGM-Ii (149, eGFP)
    Use
    AAV
    Mutation
    RGS10(truncation after amino acid 149)
    Promoter
    Human Synapsin
    Available Since
    Feb. 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    221610-plasmid-map-sequence-id-461233

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  15. polySIM

    Plasmid
    #221068
    Purpose
    Expression of polySIM protein under T7 promoter
    Depositor
    Michael Rosen
    Article
    Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33
    Insert
    10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4
    Tags
    His and MBP
    Expression
    Bacterial
    Promoter
    unknown
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    221068-plasmid-map-sequence-id-459203

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  32. MZB222

    Plasmid
    #221772
    Purpose
    Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (687-885) of Big1 (ArfGEF1) for rapid nucleotide exchange and activation of Arf GTPases
    Depositor
    Richard Baker
    Article
    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12
    Insert
    ArfGEF1 (ARFGEF1 Human)
    Tags
    10xHis-SUMO
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    221772-plasmid-map-sequence-id-455976

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  34. pCS2+-Zeo-miR-430-10-11 mm

    Plasmid
    #221727
    Purpose
    Expression of Zeocin resistance marker followed by miR-430 target site insertion with mismatches at position 10 and 11 in zebrafish embryos.
    Depositor
    David Bartel
    Article
    Chen et al Mol Cell. 2017 Dec 21;68(6):1095-1107.e5
    Insert
    BleoR-miR-430-10-11 mm
    Use
    Fish expression
    Mutation
    Mismatch at positions 10 and 11 of miR-430 site
    Promoter
    simian CMV IE94
    Available Since
    Dec. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    221727-plasmid-map-sequence-id-449656

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  40. pHelper-CRISPRi-sgRNA

    Plasmid
    #221135
    Purpose
    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    sgRNA-scaffold with dCas9 handle
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    J23119(SpeI)
    Available Since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221135-plasmid-map-sequence-id-436077

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  42. CUP1-∆ssCPY*-mScarletI

    Plasmid
    #221157
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mScarletI
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221157-plasmid-map-sequence-id-450959

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  43. CUP1-∆ssCPY*-mNeonGreen

    Plasmid
    #221156
    Purpose
    Fluorescent reporter for cytoplasmic aggregates
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mNeonGreen
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221156-plasmid-map-sequence-id-450957

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  44. CUP1-∆ssCPY*-mNeonGreen in pRS315

    Plasmid
    #221160
    Purpose
    Fluorescent reporter for cytoplasmic aggregates under LEU2 selection marker
    Depositor
    Mark Leake
    Insert
    Carboxypeptidase Y (PRC1 Budding Yeast)
    Tags
    mNeonGreen
    Expression
    Yeast
    Mutation
    Deletion signal peptide, G255A mutation
    Available Since
    Nov. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    221160-plasmid-map-sequence-id-450956

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Showing: 451 - 500 of 9696 results