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Plasmid#221956PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE C1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 alphaDdCBE C2
Plasmid#221957PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 alphaDdCBE G1
Plasmid#221958PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 alphaDdCBE G2
Plasmid#221959PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE G2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233A, See depositor commentsPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 alphaDdCBE T1
Plasmid#221960PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 alphaDdCBE T2
Plasmid#221961PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ATP6 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
CO1 DdCBE C1
Plasmid#221962PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-CO1 TALE C1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALE-free DddAtox 1397C
Plasmid#221941PurposeExpresses mitochondrially targeted TALE-free DddAtox 1397C-UGI in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-DddAtox 1397C-UGI-ATP5B 3'UTR
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALE-free DddAtox 1397N
Plasmid#221942PurposeExpresses mitochondrially targeted TALE-free DddAtox 1397N-UGI in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-DddAtox 1397N-UGI-ATP5B 3'UTR
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALE-free DddA6-11 1397C
Plasmid#221943PurposeExpresses mitochondrially targeted TALE-free DddA6/11 1397C-UGI in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-DddA6/11 1397C-UGI-ATP5B 3'UTR
UseTags3xFLAGExpressionMammalianMutationDddA: T1413IPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALE-free DddA6 1397N
Plasmid#221944PurposeExpresses mitochondrially targeted TALE-free DddA6 1397N-UGI in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-DddA6 1397N-UGI-ATP5B 3'UTR
UseTags3xFLAGExpressionMammalianMutationDddA: Q1310R, S1330I, T1380IPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
TALE-free DddA11 1397N
Plasmid#221945PurposeExpresses mitochondrially targeted TALE-free DddA11 1397N-UGI in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-DddA11 1397N-UGI-ATP5B 3'UTR
UseTags3xFLAGExpressionMammalianMutationDddA: S1330I, A1341V, N1342S, E1370K, T1380IPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 DdCBE A1
Plasmid#221946PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE A1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ATP6 DdCBE A2
Plasmid#221947PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-ATP6 TALE A2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5417_p3WJdB_promoter dropout vector
Plasmid#221191PurposePromoter probe vector with dropout sequence for promoter sequences with 3WJdB reporterDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterPromoter Placeholder/Dropout VectorAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5418_pMBP-GlcR
Plasmid#221192PurposeExpression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.DepositorInsertglcR (pden4400 from Paracoccus denitrificans)
UseTags10x His tag with E. coli maltose binding protein …ExpressionBacterialMutationPromoterT7 promoter and T7 promoter-lacOAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5419_p3WJdB-glcO36
Plasmid#221193PurposeGlcR module template with glcO36 operator: T7 promoDNA template of GlcR sensor with glcO36 operator: T7 promoter-glcO36-3WJdB reporter-T7 terminator linear DNA tter-glcO36-3WJdB reporter-T7 terminatorDepositorInsertT7 promoter-glcO36
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoter with downstream glcO36 operatorAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5423_p3WJdB-glcO60
Plasmid#221194PurposeDNA template of GlcR sensor with glcO60 operator: T7 promoter-glcO60-3WJdB reporter-T7 terminator linear DNA template (PCR amplified with oSB0021+22) is used in the IVT sensor systemDepositorInsertT7 promoter-glcO60-3WJdB
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7 promoter with downstream glcO60 operatorAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_GGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221076PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertGGG-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_CCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221077PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertCCC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SEMPER_TTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
Plasmid#221079PurposeSEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publicationDepositorInsertTTT-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT7-IVT_ACC-msfGFP[r5M]_ACC-mEBFP2
Plasmid#221082PurposePlasmid for producing DNA Templates for in vitro transcription. Produces msfGFP[r5M] and mEBFP2 from IVT mRNA.DepositorInsertACC-msfGFP[r5M]_ACC-mEBFP2
UseSynthetic BiologyTagsExpressionMutationPromoterCMV, T7Available sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pWZL-hygro-FAKS732A/S910A
Plasmid#221642PurposeThis retroviral plasmid expresses a double-mutated cDNA (dominant negative: S732A/S910A) of human PTK2DepositorInsertPTK2 (S732A/S910A) (PTK2 Human)
UseRetroviralTagsNoExpressionMammalianMutationFAK double mutation S732A/S910A (dominant negativ…PromoterAvailable sinceSept. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PD2529 human beta1 ecto
Plasmid#221398PurposeMammalian expression of human integrin beta1 ectodomainDepositorInsertintegrin beta1 ectodomain (ITGB1 Human)
UseTagsAVI tag, HRV3C cleavage site, basic coil, HA tag,…ExpressionMammalianMutationcodon optimized for human mature _1 residues Q1 t…PromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8221 pLIX-PTPN14
Plasmid#221643PurposeDoxycycline-inducible overexpression of human WT PTPN14DepositorInsertPTPN14 (PTPN14 Human)
UseLentiviralTagsHAExpressionMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8224 pLIX-PTPN14 ΔPPXY1/2
Plasmid#221644PurposeDoxycycline-inducible overexpression of human PTPN14 ΔPPXY1/2 mutantDepositorInsertPTPN14 ΔPPXY1/2 (PTPN14 Human)
UseLentiviralTagsHAExpressionMutationdeletion of the first and second (tandem) PPXY mo…PromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8552 pLIX-PTPN14 ΔPPXY3/4
Plasmid#221645PurposeDoxycycline-inducible overexpression of human PTPN14 ΔPPXY3/4 mutantDepositorInsertPTPN14 ΔPPXY3/4 (PTPN14 Human)
UseLentiviralTagsHAExpressionMutationdeletion of the third and fourth PPXY motifs as d…PromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8215 pHAGE N-V5 PTPN14 ΔPPXY1/2
Plasmid#221646PurposeConstitutive ectopic expression of human PTPN14 ΔPPXY1/2 mutantDepositorInsertPTPN14 ΔPPXY1/2 (PTPN14 Human)
UseLentiviralTagsV5ExpressionMutationdeletion of the first and second (tandem) PPXY mo…PromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8180 pHAGE N-V5 PTPN14 ΔPPXY3/4
Plasmid#221647PurposeConstitutive ectopic expression of human PTPN14 ΔPPXY3/4 mutantDepositorInsertPTPN14 ΔPPXY3/4 (PTPN14 Human)
UseLentiviralTagsV5ExpressionMutationdeletion of the third and fourth PPXY motifs as d…PromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8189 pHAGE N-V5 PTPN14 C1121S
Plasmid#221648PurposeConstitutive ectopic expression of human PTPN14 C1121S mutantDepositorInsertPTPN14 C1121S (PTPN14 Human)
UseLentiviralTagsV5ExpressionMutationC1121S mutation at the putative catalytic sitePromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8390 LentiCRISPRv2 Neo sgNT-2
Plasmid#221650PurposeExpression of non-targeting control sgRNADepositorInsertnon-targeting sgNT-2
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8391 LentiCRISPRv2 Neo sgPTPN14-1
Plasmid#221651PurposeExpression of sgRNA targeting the locus of human PTPN14DepositorInsertsgPTPN14-1 (PTPN14 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p8392 LentiCRISPRv2 Neo sgPTPN14-3
Plasmid#221652PurposeExpression of sgRNA targeting the locus of human PTPN14DepositorInsertsgPTPN14-3 (PTPN14 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-FLAG-msMMS22L
Plasmid#221015Purposemammalian expression vector of Flag tagged mouse MMS22LDepositorInsertMMS22L (Mms22l Mouse)
UseTagsFlagExpressionMammalianMutationnaturally siResistant to siMMS22L (human) aagacuu…PromoterCMVAvailable sinceSept. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/TO-eGFP-msMMS22L
Plasmid#221014Purposemammalian expression vector of eGFP tagged mouse MMS22LDepositorInsertMMS22L (Mms22l Mouse)
UseTagseGFPExpressionMammalianMutationnaturally siResistant to siMMS22L (human) aagacuu…PromoterCMVAvailable sinceSept. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV:: NES-FKBP-iLID-LRP6c(△1-64)
Plasmid#221775PurposeExpresses NES-FKBP-iLID-LRP6c(△1-64) component in mammalian cellsDepositorInsertpCMV:: NES-FKBP-iLID-LRP6c
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
TCT-Universal plasmid
Plasmid#221852PurposeA universal plasmid for expressing any protein with TetraCysteine motifDepositorInsertTetra Cysteine tag
UseTagsExpressionBacterialMutationNoPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
eSrtA-Δ24-ASN
Plasmid#221849PurposeExpresses delta 24 alpha synuclein as 6XHis-SUMO-eSrtA-LPNTG-Δ24-ASN fusion proteinDepositorInsertalpha-synuclein (SNCA Human)
UseTagsExpressionBacterialMutationNoPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28-Butelase
Plasmid#221848PurposeExpresses asparaginyl endopeptidase butelaseDepositorInsertButelase 1 ligase
UseTagsExpressionBacterialMutationNoPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ub-LPNTG-pET
Plasmid#221850PurposeExpresses Ub with sortase motif and His tagDepositorInsertUbiquitin
UseTagsExpressionBacterialMutationArg74ProLeuThrPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUMO-LPQTG-pET
Plasmid#221851PurposeExpresses SUMO-2 with sortase motif and His tagDepositorInsertSUMO-2 (SUMO2 Human)
UseTagsExpressionBacterialMutationGln88Leu, Gln89ProPromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-Spo20(PABD)
Plasmid#221758PurposeStable expression of GFP-Spo20(PABD) in mammalian cellsDepositorInsertSpo20 (SOS1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem Spo20(PABD) (amino acids 422-551)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-TAPP1(PHD)
Plasmid#221757PurposeStable expression of GFP-TAPP1(PHD) in mammalian cellsDepositorInsertPLEKHA1 (PLEKHA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem TAPP1 (PHD) (amino acids 184-304)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-Btk1(PHD)-GFP
Plasmid#221756PurposeStable expression of Btk1(PHD)-GFP in mammalian cellsDepositorInsertBtk (BTK Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–177PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-PKD C1ab
Plasmid#221755PurposeStable expression of GFP-PKD C1ab in mammalian cellsDepositorInsertPrkd1(138-343) (Prkd1 Mouse)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 138-343PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-Evectin-2
Plasmid#221754PurposeStable expression of Evectin-2 in mammalian cellsDepositorInsertPLEKHB2 (PLEKHB2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem PLEKHB2(PH domain) (amino acids 1-110)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-PLCd1(PHD)
Plasmid#221753PurposeStable expression of GFP-PLCd1(PHD) in mammalian cellsDepositorInsertPLCd1 (PLCD1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationamino acids 1–175PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-TRPML1(PHD)
Plasmid#221752PurposeStable expression of GFP-TRPML1(PHD) in mammalian cellsDepositorInsertTRPML1 (MCOLN1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem TRPML1(PHD) (amino acids 1–69)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-ING2(PHD)
Plasmid#221751PurposeStable expression of GFP-ING2(PHD) in mammalian cellsDepositorInsertING2 (ING2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtwo ING2(PlantHD) domains (amino acids 190–280)PromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-2xFYVE
Plasmid#221750PurposeStable expression of GFP-2xFYVE in mammalian cellsDepositorInserttandem HRS FYVE domain
UseRetroviralTagsGFPExpressionMammalianMutationtwo FYVE domains (amino acids 147-223), linked by…PromoterAvailable sinceAug. 26, 2024AvailabilityAcademic Institutions and Nonprofits only