We narrowed to 12,313 results for: 112

1. RhoA sensor (LSS-SGFP2-mCherry)

   Plasmid

   #112948

   Purpose

   RhoA FRET biosensor

   Depositor

   Dorus Gadella

   Insert

   Rhoa sensor
   

   Tags

   LSS-SGFP2 and mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2

   Plasmid

   #112837

   Purpose

   transient/stable overexpression

   Depositor

   Tomasz Prószyński

   Article

   Gingras et al J Cell Sci. 2016 Mar 1;129(5):898-911. d

   Insert

   Grb2 (Grb2 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   SH2 + SH3 (C-term) domains of Grb2

   Available Since

   Aug. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2

   Plasmid

   #112004

   Purpose

   For enriched expression of GCaMP6s in axons with cytosolic expression of mKate2

   Depositor

   Lin Tian

   Article

   Broussard et al Nat Neurosci. 2018 Sep;21(9):1272-1280.

   Has Service

   AAV9

   Insert

   axon-GCaMP6s-P2A-mKate2
   

   Use

   AAV

   Tags

   GAP43 palmitoylation domain

   Expression

   Mammalian

   Promoter

   hSynapsin1

   Available Since

   June 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ubiq-Cas9.874W

   Plasmid

   #112686

   Purpose

   Express hSpCas9 under Ubiquitin-63E promoter

   Depositor

   Omar Akbari

   Article

   Kandul et al Nat Commun. 2019 Jan 8;10(1):84. doi: 10

   Insert

   hSpCas9
   

   Tags

   3x FLAG, EGFP, and NLS

   Expression

   Insect

   Mutation

   Humanized Cas9 bacterial sequence

   Promoter

   Ubiquitin-63E promoter

   Available Since

   Jan. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pNR4A1.1.0-gDNA

   Plasmid

   #112391

   Purpose

   CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1

   Depositor

   Kevin White

   Insert

   NR4A1 (NR4A1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQE80L SpyTag-ELP-SpyTag

   Plasmid

   #112634

   Purpose

   Two SpyTags linked by an elastin-like polypeptide for hydrogel formation

   Depositor

   Mark Howarth

   Article

   Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

   Insert

   SpyTag-ELP-SpyTag
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   central cysteine

   Promoter

   T5

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-FLAG-RNMT

   Plasmid

   #112705

   Purpose

   Expresses N-terminally FLAG-tagged human RNMT in mammalian cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

   Insert

   RNMT (RNMT Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUWL201PW-creDEM

   Plasmid

   #112242

   Purpose

   Streptomyces/E.coli shuttle expression vector encoding creD, creE, creM

   Depositor

   Emily Balskus

   Article

   Waldman et al J Org Chem. 2018 May 29. doi: 10.1021/ac

   Inserts

   creD
   creE
   CreM
   

   Available Since

   June 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLBH545_Tet-Cas14a1_Locus

   Plasmid

   #112501

   Purpose

   Expresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14a1
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   Tet

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLJM1 TMEM192-Flag-FKBP

    Plasmid

    #112743

    Purpose

    express TMEM192-Flag-FKBP

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    TMEM192-Flag-FKBP (TMEM192 Human)
    

    Use

    Lentiviral

    Tags

    Flag, FKBP

    Available Since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKLV2-U6gRNASAM(gEmpty)-TREGFP-PGKpuroBFP-W

    Plasmid

    #112926

    Purpose

    CRISPR-a reporter assay lentiviral vector (empty gRNA control)

    Depositor

    Kosuke Yusa

    Article

    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

    Insert

    hU6-gRNASAM(empty), TREGFP, PGKpuroGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AdML

    Plasmid

    #11242

    Depositor

    Robin Reed

    Article

    Luo et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

    Insert

    AdML
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2006

    Availability

    Academic Institutions and Nonprofits only

13. pMAL-Abl-PRM 4R

    Plasmid

    #112087

    Purpose

    Bacterial expression plasmid containing His and MBP tags for 4 PRM motif repeats of Human Abl.

    Depositor

    Michael Rosen

    Article

    Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

    Insert

    Abl-PRM-4R (ABL1 Human)
    

    Tags

    His-6 and MBP

    Expression

    Bacterial

    Mutation

    construct contains only the PRM motif of Abl1

    Promoter

    Lac

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBP-Pspac

    Plasmid

    #112778

    Purpose

    Promoter repressed by LacI (not natively produced in B. subtilis 168)

    Depositor

    Chris French

    Article

    Wicke et al bioRxiv 185108

    Insert

    Pspac promoter
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMTB

    Plasmid

    #112225

    Purpose

    Expression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.

    Depositor

    Sean Megason

    Article

    Wagner et al Science. 2018 Apr 26. pii: science.aar43

    Insert

    Super-folder GFP
    

    Use

    Zebrafish expression

    Promoter

    beta actin 2 (zebrafish)

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX-sgRNA-BfuAI-2k

    Plasmid

    #112915

    Purpose

    Empty sgRNA expression plasmid

    Depositor

    Ren-Jang Lin

    Article

    Kurata et al RNA. 2018 May 2. pii: rna.066282.118. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHis-parallel1-NLSH2BCas9

    Plasmid

    #112065

    Purpose

    Expression and purification of NLSH2BCas9 for fungal gene editing

    Depositor

    Jeff Coleman

    Article

    Wang et al Fungal Genet Biol. 2018 May 12. pii: S10

    Insert

    NLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
    

    Use

    CRISPR

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Cas9 was codon optimized for Aspergillus niger

    Promoter

    promoter in vector

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pExp-Bla

    Plasmid

    #112561

    Purpose

    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and Bla (beta-lactamase from the halophile Chromohalobacter sp. fusions with optional C-terminal StrepII-tag

    Depositor

    Marko Hyvönen

    Type

    Empty backbone

    Tags

    8xHis-Bla and Strep tag

    Expression

    Bacterial

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV_AncBE4max_3xHA

    Plasmid

    #112097

    Purpose

    C:G-to-T:A base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2018 May 29. pii: nbt.41

    Insert

    AncBE4max_3xHA
    

    Use

    CRISPR

    Tags

    3xHA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLL3.7-EF-EYFP-YAP1_S94A-PolyA

    Plasmid

    #112286

    Purpose

    Fluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutant

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Mutation

    Human YAP1-2gamma with Serine 94 to Alanine

    Promoter

    EF-1 alpha

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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