We narrowed to 12,769 results for: 112
-
Plasmid#112915PurposeEmpty sgRNA expression plasmidDepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only
-
pGEX SH3(2)-3R
Plasmid#112091PurposeBacterial expression plasmid containing a GST tag with 3 repeats of the second SH3 domain from human NCK1.DepositorAvailable SinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV_AncBE4max_3xHA
Plasmid#112097PurposeC:G-to-T:A base editingDepositorInsertAncBE4max_3xHA
UseCRISPRTags3xHAExpressionMammalianPromoterCMVAvailable SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pQE80L SpyTag-ELP-SpyTag
Plasmid#112634PurposeTwo SpyTags linked by an elastin-like polypeptide for hydrogel formationDepositorInsertSpyTag-ELP-SpyTag
TagsHis6ExpressionBacterialMutationcentral cysteinePromoterT5Available SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
CMV-mEGFP-PKCa-C1
Plasmid#112264PurposeITRACKalpha Donor ConstructDepositorInsertprotein kinase C alpha (PRKCA Bovine)
TagsmEGFPExpressionMammalianMutationA11V (please see depositor comments below)PromoterCMVAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2
Plasmid#112004PurposeFor enriched expression of GCaMP6s in axons with cytosolic expression of mKate2DepositorHas ServiceAAV9Insertaxon-GCaMP6s-P2A-mKate2
UseAAVTagsGAP43 palmitoylation domainExpressionMammalianPromoterhSynapsin1Available SinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)/Puro/FA/GFP-N--Grb2-2
Plasmid#112837Purposetransient/stable overexpressionDepositorAvailable SinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3 FLAG_CMV14/B0,+
Plasmid#112798PurposeExpression of ATB0,+DepositorAvailable SinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNR4A1.1.0-gDNA
Plasmid#112391PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1DepositorAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-GST-eIF4E K119A
Plasmid#112818PurposeExpresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cellsDepositorInsertEif4e (Eif4e Mouse)
TagsGSTExpressionBacterialMutationK119 mutated to A, increasing affinity for cap st…PromoterT7Available SinceJan. 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
LUC01 (L/Sec/P)
Plasmid#112144PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
ExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U)Available SinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Ubiq-Cas9.874W
Plasmid#112686PurposeExpress hSpCas9 under Ubiquitin-63E promoterDepositorInserthSpCas9
Tags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoterUbiquitin-63E promoterAvailable SinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
p3 FLAG_CMV14/OCTN2 (wt)
Plasmid#112799PurposeExpression of OCTN2DepositorAvailable SinceAug. 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_S94A-PolyA
Plasmid#112286PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) S94A mutantDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma with Serine 94 to AlaninePromoterEF-1 alphaAvailable SinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKD4-Nter-APEX2
Plasmid#112868PurposeBacterial plasmid derived from pKD4 for chromosomal insertion of APEX2 gene in 5' of a gene of interest by using Datsenko & Wanner methodDepositorInsertAPEX2
Available SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pExp-mCherry
Plasmid#112569PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and mCherry (mCherry fluorescent protein,) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneTags8xHis, Strep II, and mCherryExpressionBacterialAvailable SinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RNMT
Plasmid#112705PurposeExpresses N-terminally FLAG-tagged human RNMT in mammalian cellsDepositorAvailable SinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-GCaMP6s-P2A-mKate2
Plasmid#112006PurposeFor co-expression of GCaMP6s with mKate2DepositorHas ServiceAAV9InsertGCaMP6s-P2A-mKate2
UseAAVExpressionMammalianPromoterhSynapsin1Available SinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP4 ermC
Plasmid#112884PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Plac promoter.DepositorAvailable SinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMTB
Plasmid#112225PurposeExpression of membrane-tagged super folder GFP from zebrafish beta-actin promoter.DepositorInsertSuper-folder GFP
UseZebrafish expressionPromoterbeta actin 2 (zebrafish)Available SinceJuly 30, 2018AvailabilityAcademic Institutions and Nonprofits only