1. p426 103Q GPD

   Plasmid

   #1184

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. pGEX-4T3-RhoA-Q63L

   Plasmid

   #12961

   Depositor

   Gary Bokoch

   Insert

   RhoA constitutively active (RHOA Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Q63L Constitutively active

   Promoter

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

2. R777-E277 Hs.RHOA

   Plasmid

   #70561

   Purpose

   Gateway ORF clone of human RHOA [NM_001664.2] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   RHOA (RHOA Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ARF5

   Plasmid

   #25530

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   ARF5 (ARF5 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 24, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

4. RHOAA-c035

   Plasmid

   #205118

   Purpose

   RHOA protein expression

   Depositor

   Paul Brennan

   Insert

   RHOA (RHOA Human)
   

   Use

   Tags

   6His-TEV and AviTag

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RHOAA-c034

   Plasmid

   #205117

   Purpose

   RHOA protein expression

   Depositor

   Paul Brennan

   Insert

   RHOA (RHOA Human)
   

   Use

   Tags

   6His-TEV

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   Available since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET3-ARF6-His6(CO)

   Plasmid

   #201887

   Purpose

   Used for bacterial expression of human protein ARF6

   Depositor

   Richard Kahn

   Insert

   ARF6 (ARF6 Human)
   

   Use

   Tags

   6xHis (GSGSGSHHHHHH)

   Expression

   Bacterial

   Mutation

   Codon optimized for bacterial expression

   Promoter

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET3-ARF4-His6(CO)

   Plasmid

   #201885

   Purpose

   Used for bacterial expression of human protein ARF4

   Depositor

   Richard Kahn

   Insert

   ARF4 (ARF4 Human)
   

   Use

   Tags

   6xHis (GSGSGSHHHHHH)

   Expression

   Bacterial

   Mutation

   Codon optimized for bacterial expression

   Promoter

   Available since

   Aug. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET3-ARF3-His6(CO)

   Plasmid

   #201884

   Purpose

   Used for bacterial expression of human protein ARF3

   Depositor

   Richard Kahn

   Insert

   ARF3 (ARF3 Human)
   

   Use

   Tags

   6xHis (GSGSGSHHHHHH)

   Expression

   Bacterial

   Mutation

   Codon optimized for bacterial expression

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pmiRNA1-OE-METTL16-sgMETTL16-MUT1

   Plasmid

   #196206

   Purpose

   overexpress mutant METTL16

   Depositor

   Jianjun Chen

   Insert

   Synonymous mutation of METTL16 ORF (METTL16 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Synonymous mutation

   Promoter

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pmiRNA1-OE-METTL16 F187G-sgMETTL16-MUT1

    Plasmid

    #196201

    Purpose

    overexpress METTL16

    Depositor

    Jianjun Chen

    Insert

    METTL16 F187G (METTL16 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    F187G

    Promoter

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pmiRNA1-OE-METTL16-sgMETTL16-MUT2

    Plasmid

    #196202

    Purpose

    overexpress METTL16

    Depositor

    Jianjun Chen

    Insert

    Synonymous mutation of METTL16 ORF (METTL16 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Synonymous mutation

    Promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. R777-E278 Hs.RHOA-nostop

    Plasmid

    #70562

    Purpose

    Gateway ORF clone of human RHOA [NM_001664.2] without stop codon (for C-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RHOA (RHOA Human)
    

    Use

    Gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. IDG_CACNG1_OE_1

    Plasmid

    #161650

    Purpose

    Overexpresses CACNG1

    Depositor

    Mike McManus

    Insert

    CACNG1 (CACNG1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. ARF4

    Plasmid

    #25520

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ARF4 (ARF4 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 24, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

15. pmiRNA1-OE-METTL16 PP185/186AA-sgMETTL16-MUT1

    Plasmid

    #196205

    Purpose

    overexpress mutant METTL16

    Depositor

    Jianjun Chen

    Insert

    METTL16 PP185/186AA (METTL16 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    PP185/186AA

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDEST17-UbE2J2

    Plasmid

    #15794

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2J2 (UBE2J2 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

17. ARF1

    Plasmid

    #28168

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    ARF1 (ARF1 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 28, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

18. RHOA

    Plasmid

    #73231

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    RHOA (RHOA Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Bacterial

    Mutation

    pfam00071:Ras 7-180

    Promoter

    T7

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. METT10D

    Plasmid

    #25485

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    METT10D (METTL16 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 23, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pE2F3-donor

    Plasmid

    #113809

    Purpose

    CRISPR donor plasmid to tag human transcription factor E2F3 with GFP

    Depositor

    Kevin White

    Insert

    E2F3 homology arms flanking EGFP-IRES-Neo cassette (E2F3 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Unknown

    Promoter

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge