-
Plasmid#186932PurposeGenomic targeting of SNAP tag at Hist1h3g C-terminalDepositorInsertSNAP donor for Hist1h3g (H3c8 Mouse)
UseTagsSNAPExpressionMammalianMutationPromoterT7Available sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pESC-LEU-IpgD(C439S)
Plasmid#183673PurposeInducible Shigella flexneri IpgD (catalytically inactive) for expression in yeastDepositorInsertIpgD
UseTagsExpressionYeastMutationC439SPromoterGAL1Available sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo(Orange)
Plasmid#140157PurposeExpresses a regulatable secretory cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo(Orange)
Plasmid#140158PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac128-pOst1-ESCargo(Crimson)
Plasmid#140156PurposeExpresses a regulatable secretory cargo for yeast at the LEU2 locusDepositorInsertpOst1-APVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIplac204-pOst1-ESCargo(Crimson)
Plasmid#140154PurposeExpresses a vacuolar cargo for yeast at the TRP1 locusDepositorInsertpOst1-APVNTT-DsRed-Express2-FKBP(FV)C22V-QRPL
UseTagsExpressionYeastMutationPromoterTPI1Available sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
Y365A or A365Y inducible rescue
Plasmid#131323PurposegRNA to generate i-WT-r or i-MT-r systemsDepositorInsertY365A or A365Y inducible rescue
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Rescue myc-PCDHGC5 mRNA
Plasmid#122229PurposeRescue mRNA for Protocadherin gamma C5 knock down with sh1 shRNA (plasmid 122227). With five silent mutations at sh1 shRNA target site.DepositorInsertPCDHGC5 (Pcdhgc5 Rat)
UseTags9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…ExpressionMammalianMutationSilent mutations are in five consecutive codons …PromoterAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pESC/mCitrine/erg3-mCherry
Plasmid#117993PurposeCo-expression of mCitrine under the control of the Gal1 promoter and erg3 fused to mCherry (an ER-targeting marker protein) under the control of the Gal10 promoterDepositorInserterg3-mCherry
UsePlant/bacteria binary vectorTagsExpressionPlantMutationPromoterGal10Available sinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
Torso 11.5 kb rescue fragment
Plasmid#39810DepositorInsertTorso genomic DNA fragment (tor Fly)
UseTagsExpressionMutationPromoterAvailable sinceOct. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-GalNAc-T2-GFP-ESCargo(FTV)
Plasmid#140163PurposeExpresses a regulatable secretory cargo for mammalian cells and a Golgi markerDepositorInsertGalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Human, Synthetic)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Crimson)
Plasmid#140162PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Orange)
Plasmid#140161PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
UseTagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac (GST-preSci site-CD45)
Plasmid#177889PurposeBaculo expression of GST tag fused to human CD45 ICDDepositorInsertCD45 (aa 598-1304 only) (PTPRC Human)
UseTagsTEV-GST-PreScission cut siteExpressionInsectMutationL652PPromoterPolyhedrinAvailable sinceAug. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pESC-URA-Ub-G76A-Rnq1-GFP
Plasmid#21052DepositorInsertRnq1 (RNQ1 Budding Yeast)
UseYeast/bacteria shuttleTagsGFP and ubiquitinExpressionYeastMutationPromoterAvailable sinceMay 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
Flag-TRIM24-F979A/N980A shRescue
Plasmid#28143DepositorInsertTRIM24 (TRIM24 Human)
UseTagsFLAGX2ExpressionMammalianMutationA489V, F979A and N980A; aa 308 and 309 mutated fo…PromoterAvailable sinceFeb. 10, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5 HA-GST-Presc-mAkt1 (S473T)
Plasmid#48806Purposeexpresses mutant Akt1DepositorInsertAkt1 (Akt1 Mouse)
UseTagsHA-GST-PrescExpressionMammalianMutationS473TPromoterCMVAvailable sinceMay 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
HOXB13 (human) Myc-tag-Bluescript
Plasmid#8684DepositorInsertHOXB13 (HOXB13 Human)
UseTagsMycExpressionBacterialMutationMyc tag on N-terminalPromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBABE-EGFP-THAP11-rescue-3xFLAG
Plasmid#37000DepositorInsertTHAP11 (THAP11 Human)
UseRetroviralTags3xFLAGExpressionMammalianMutationMutations were generated at codons 273-275 conver…PromoterAvailable sinceMay 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
B. adolescentis mRNA toehold switch sensor
Plasmid#110710PurposeToehold switch sensor to detect a species specific mRNA with GFP outputDepositorInsertB. adolescentis species specific toehold switch sensor
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only