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We narrowed to 238 results for: LbCas12a

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Showing: 181 - 200 of 238 results

  1. pCRI007-pGEM-PgpdA-Cas12aScaffold-BsmbI-TtrpC

    Plasmid
    #140200
    Purpose
    Cloning vector for LbCas12a crRNAs ( Step 1 of the 2-step cloning alternative). Full PgpdA sequence is reconstituted when amplifying the expression cassette for cloning in a fungal vector.
    Depositor
    Yit Heng Chooi
    Publication
    Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Tags
    dLbCas12a crRNA scaffold
    Promoter
    PgpdA
    Available since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    140200-plasmid-map-sequence-id-310179

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  2. WN10151

    Plasmid
    #80441
    Purpose
    Expresses human codon-optimized dead (832A) LbCpf1
    Depositor
    Ervin Welker
    Publication
    Toth et al Biol Direct. 2016 Sep 14;11:46. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead (D832A) LbCpf1
    Use
    CRISPR
    Tags
    NLS-3xHA
    Expression
    Mammalian
    Mutation
    D832A
    Available since
    Aug. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    80441-plasmid-map-sequence-id-151446

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  3. pY31

    Plasmid
    #84746
    Purpose
    Expresses huLbCpf1-P2A-puro and crRNA guide
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    huLbCpf1
    puromycin resistance gene
    Use
    CRISPR
    Tags
    3xHA, NLS, and T2A
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    84746-plasmid-map-sequence-id-162433

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  5. pSLQ8547 PB-PGK-dLbCpf1-2XNLS-miniVPR-mCherry-pA

    Plasmid
    #140220
    Purpose
    Constitutive PGK driven expression of full length dCpf1 (Cas12a) -miniVPR activator
    Depositor
    Stanley Qi
    Publication
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1
    Use
    CRISPR
    Tags
    miniVPR-mCherry
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    PGK
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140220-plasmid-map-sequence-id-291993

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  6. YET1000: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X4)

    Plasmid
    #104571
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to four DmrA domains
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ‘dead’ Cpf1 fused to four DmrA domains
    Use
    CRISPR
    Tags
    NLS-3xHA-3xFLAG-4xDmrA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    104571-plasmid-map-sequence-id-201436

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  7. MMW1578: CAG-human dLbCpf1(D832A)-NLS-3xHA

    Plasmid
    #104563
    Purpose
    Mammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1)
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2017 Oct 30. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1 (D832A)
    Use
    CRISPR
    Tags
    NLS-3xHA
    Expression
    Mammalian
    Mutation
    D832A
    Promoter
    CAG
    Available since
    Jan. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    104563-plasmid-map-sequence-id-260085

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  13. pLCHKO_HPRT1_exon2_1_Lb

    Plasmid
    #155051
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155051-plasmid-map-sequence-id-308437

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  14. pYPQAT52

    Plasmid
    #223424
    Purpose
    T-DNA vector for dLbCas12a-D156R mediated A to G base editing for monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were driven by separate ZmUbi1 promoter; BASTA for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-dLbCas12a-D156R-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223424-plasmid-map-sequence-id-454179

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  15. pYPQAT53

    Plasmid
    #223425
    Purpose
    T-DNA vector for dLbCas12a-D156R mediated A to G base editing for monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were driven by separate ZmUbi1 promoter; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-dLbCas12a-D156R-ZmUbi-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223425-plasmid-map-sequence-id-454170

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  16. pYPQAT54

    Plasmid
    #223426
    Purpose
    T-DNA vector for dLbCas12a-D156R mediated A to G base editing for monocot plants; TTTV PAM; LbCas12a-D156R-ABE and the crRNA were driven by separate 2x35s promoter; Kanamycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-ecTadA8e-dLbCas12a-D156R-2x35s-crRNA scaffold
    Use
    CRISPR
    Tags
    NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223426-plasmid-map-sequence-id-452223

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  17. pLCHKO_HPRT1_exon3_2_Lb

    Plasmid
    #155054
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 3
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155054-plasmid-map-sequence-id-308516

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  18. pLCHKO_HPRT1_exon3_1_Lb

    Plasmid
    #155053
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 3 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 3
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155053-plasmid-map-sequence-id-308427

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  19. pLCHKO_HPRT1_exon2_2_Lb

    Plasmid
    #155052
    Purpose
    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of HPRT1 exon 2 using SpCas9 and LbCas12a nucleases (CHyMErA system)
    Depositor
    Benjamin Blencowe, Jason Moffat
    Publication
    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.
    Insert
    (hg)RNA for deletion of HPRT1 exon 2
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Aug. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    155052-plasmid-map-sequence-id-308434

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  20. pYPQ233-STU

    Plasmid
    #138111
    Purpose
    Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated LbCas12a-SRDX repressor without promoter and terminator
    Depositor
    Yiping Qi
    Publication
    Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:
    Insert
    dLbCas12a-SRDX
    Use
    CRISPR; Gateway compatible cas12a entry clone
    Tags
    NLS
    Expression
    Plant
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    138111-plasmid-map-sequence-id-272043

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Showing: 181 - 200 of 238 results