We narrowed to 12,552 results for: NUC

1. pOTTC1617 pAAV SYN1 Nuc-EYFP

   Plasmid

   #135567

   Purpose

   An AAV vector expressing a neuronally expressed nuclear EYFP reporter

   Depositor

   Christopher Richie

   Article

   Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

   Inserts

   empty
   Nuc-EYFP
   

   Use

   AAV

   Tags

   3xNLS

   Expression

   Mammalian

   Mutation

   Promoter

   hSYN1

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLV-REPORT(EF1a no GFP) mnuctdTomato

   Plasmid

   #172342

   Purpose

   mnuctdTomato

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Inserts

   monomeric nuclear Tomato
   HygR
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKK-RNAi-nucEGFPmiR-mCherry-TEV

   Plasmid

   #105808

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal mCherry.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   mCherry-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKK-RNAi-nucEGFPmiR-FLAG-TEV

   Plasmid

   #105807

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear EGFP expression marker; your protein of interest with N-terminal FLAG tag.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   FLAG-TEV

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKK-RNAi-nucCHERRYmiR-TEV-FLAG

   Plasmid

   #105813

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal FLAG.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   TEV-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKK-RNAi-nucCHERRYmiR-TEV-EGFP

   Plasmid

   #105814

   Purpose

   Functional studies using RNAi; rescue experiments; substituting protein for its different form. miRNA cassette with nuclear mCherry expression marker; your protein of interest with C-terminal EGFP.

   Depositor

   Andrzej Dziembowski

   Article

   Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

   Type

   Empty backbone

   Use

   RNAi; Flp-in competent

   Tags

   TEV-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETcon-pGAL1-Aga2-α-Synuclein-c_myc

   Plasmid

   #225222

   Purpose

   Yeast optimized human alpha synuclein fused with aga2 protein gene under gal1 promoter for the expression of alpha synuclein in yeast surface display.

   Depositor

   Urartu Seker

   Article

   Ozcelik et al ACS Chem Neurosci. 2023 Oct 4;14(19):360

   Inserts

   α-Synuclein
   Aga2
   

   Use

   Tags

   GS linker, HA Tag, and c-myc Tag

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. SARS-CoV-2 Nucleocapsid-3xHA-IRES-mcherry

   Plasmid

   #173030

   Purpose

   To express SARS-CoV-2 Nucleocapsid protein tagged with 3xHA and in frame with mcherry (NCP-IRES-mcherry) in mammalian cells

   Depositor

   Mohamed Fareh

   Article

   Fareh et al Nat Commun. 2021 Jul 13;12(1):4270. doi:

   Insert

   SARS-CoV-2 Nucleocapsid phosphoprotein
   

   Use

   Lentiviral

   Tags

   3xHA and IRES-mcherry

   Expression

   Mammalian

   Mutation

   Promoter

   Gag (truncated)

   Available since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre

   Plasmid

   #112683

   Purpose

   An AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase

   Depositor

   Brandon Harvey

   Article

   Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

   Insert

   iRFP713
   

   Use

   AAV

   Tags

   2A-iCre and Nuclear localization signal

   Expression

   Mutation

   Promoter

   CMV-IE

   Available since

   March 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CMVenh synapsin-intron-aSYNUCLEIN A53T

    Plasmid

    #227004

    Purpose

    AAV transfer plasmid encoding the human A53T mutant α-SYN under the control of the CMVie enhanced synapsin1 promoter

    Depositor

    Veerle Baekelandt

    Article

    Van der Perren et al J Vis Exp. 2016 Feb 28;(108):53670. doi:

    Insert

    alpha synuclein A53T mutant (SNCA Human, Synthetic)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    A53T

    Promoter

    CMVenh synapsin

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOTTC1012 - pAAV EF1a DIO Nuc-eYFP

    Plasmid

    #75082

    Purpose

    An AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.

    Depositor

    Brandon Harvey

    Article

    Sharpe et al Curr Biol. 2017 Jul 24;27(14):2089-2100.

    Insert

    Nuc-eYFP
    

    Use

    AAV and Cre/Lox

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid

    #114089

    Purpose

    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized FnCas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)

    Plasmid

    #115142

    Purpose

    CAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver, Joseph Bondy-Denomy

    Article

    Marino et al Science. 2018 Sep 6. pii: science.aau517

    Insert

    human codon optimized Mb3Cas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo

    Plasmid

    #112684

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-Flpo and Nuclear localization signal

    Expression

    Mutation

    Promoter

    CMV-IE

    Available since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Myopathy Loop Mutant Nuclear Myosin I YFP

    Plasmid

    #60243

    Purpose

    Expresses a myopathy loop mutant (6 mutations) of the nuclear isoform of myosin IC attached to YFP and an NLS, which has impaired actin binding activity

    Depositor

    Primal de Lanerolle

    Article

    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

    Insert

    Nuclear Myosin 1 (Myo1c Mouse)
    

    Use

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Mutation

    Deleted I339, I340, G343, E344, E345,L346

    Promoter

    CMV

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

16. PEA1-Nuclease-Puro HEK3 CTT ins

    Plasmid

    #171995

    Purpose

    Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid

    Depositor

    Paul Thomas

    Article

    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

    Insert

    HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV for Cas9, U6 for gRNAs

    Available since

    Sept. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-hMbCas12a-NLS(nucleoplasmin)-3xHA (AAS2134)

    Plasmid

    #114090

    Purpose

    CAG promoter expression plasmid for human codon optimized MbCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized MbCas12a
    

    Use

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. PcDNA-DEST47-CypB-Nucl2 C-term GFP

    Plasmid

    #36126

    Depositor

    Patrick Van Oostveldt

    Article

    Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

    Insert

    cyclophilin B (PPIB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains CypB AA 34-211

    Promoter

    CMV

    Available since

    Nov. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

19. PcDNA-DEST47-CypB-Nucl C-term GFP

    Plasmid

    #36124

    Depositor

    Patrick Van Oostveldt

    Article

    Dieriks et al Int J Mol Med. 2012 Mar 13. doi: 10.3892

    Insert

    Cyclophilin B (PPIB Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains amino acids 34-216 of cyclophilin B

    Promoter

    CMV

    Available since

    Sept. 27, 2012

    Availability

    Academic Institutions and Nonprofits only

20. Nuc-Orange Nano-lantern(Ca2+)/pcDNA3

    Plasmid

    #71349

    Purpose

    Express Nuc-Orange Nano-lantern-based luminescent Ca2+ indicator in mammalian cel

    Depositor

    Takeharu Nagai

    Article

    Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

    Insert

    Orange Nano-lantern(Ca2+)_H2B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 8, 2015

    Availability

    Academic Institutions and Nonprofits only