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Plasmid#103311PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-195-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-195-3p target (MIR195 Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK299
Plasmid#59568PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) Kozak L7Ae-P2A-EYFP 4xFF4DepositorInsertSGP(14) Kozak L7Ae-P2A-EYFP 4xFF4
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a(cd226)
Plasmid#177900PurposeBacterial expression of his10-tagged human cd226 Intracellular DomainDepositorInsertCD226 (aa 288-336 only) (CD226 Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceApril 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCGN-ELK-1 S383A
Plasmid#27160PurposeFor mammalian expression of human Elk-1 with an HA tag and a mutation in the MAPK phosphorylation site of the transcriptional activation domainDepositorInsertELK1 (ELK1 Human)
UseTagsHAExpressionMammalianMutationTAD mutant: S383APromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-29a-3p
Plasmid#103391PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29a-3p target (MIR29A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-181a-5p
Plasmid#103276PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-181a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-181a-5p target
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJB332
Plasmid#115949PurposeORF insert for replicon MoCloDepositorInsertLvl0 TetR-Gly9-DDX6 (contains SapI)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTW122
Plasmid#115962PurposeORF insert for replicon MoCloDepositorInsertLvl0 5'-2xTetApt-mVenus-PEST
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
hPDI1-18-508_C400S_C56S-pFLAG-CMV1
Plasmid#31383DepositorInsertPDI1A (P4HB Human)
UseTagsFLAG M2ExpressionMammalianMutationC-terminal cysteines of both active sites mutated…PromoterAvailable sinceMay 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac (GST-preSci site-CD45)
Plasmid#177889PurposeBaculo expression of GST tag fused to human CD45 ICDDepositorInsertCD45 (aa 598-1304 only) (PTPRC Human)
UseTagsTEV-GST-PreScission cut siteExpressionInsectMutationL652PPromoterPolyhedrinAvailable sinceAug. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTK332
Plasmid#59573PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5DepositorInsertSGP(14) 2xK-turn Kozak MS2-CNOT7 (SacI mutated)-P2A-EBFP2-4xFF5
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
DNA-CARtcr-IRESpuro
Plasmid#89346Purpose2nd generation lentiviral vector which expresses SNAP tagged TCRbeta subunit upstream of a IRESpuro cassetteDepositorInsertTCR beta gene (Jurkat allele) fused at the N terminus to the SNAP tag
UseLentiviralTagsSNAPf tagExpressionMammalianMutationPromoterSFFVAvailable sinceJune 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a(TIGIT)
Plasmid#177902PurposeBacterial expression of his10-tagged human TIGIT Intracellular DomainDepositorInsertTIGIT (aa 163-244 only) (TIGIT Human)
UseTags10xHis-Thrombin cut site-T7 tagExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-ZIP(WT)
Plasmid#27134DepositorUseLentiviralTagseGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1-MKL1/2 shRNA
Plasmid#27161PurposeLentiviral shRNA vector for knockdown of both mouse MKL1 and MKL2.DepositorInsertshRNA MKL1+2
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
hPDI1-18-508_C400S-pFLAG-CMV1
Plasmid#31384DepositorInserthPDI1 (P4HB Human)
UseTagsFLAG M1ExpressionMammalianMutationC400SPromoterAvailable sinceMay 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
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LSB-hsa-miR-29a-5p
Plasmid#103392PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-29a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-29a-5p target (MIR29A Human)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterEF-1aAvailable sinceDec. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTK105
Plasmid#59562PurposeSIN SP6 P1234 (nsP2 P726S) SGP(14) 2xK-turn EYFP-PESTDepositorInsertSGP(14) 2xK-turn EYFP-PEST
UseTagsExpressionMutationPromoterAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
MyD116.delC.pBABEpu
Plasmid#21835DepositorInsertGADD34 (Ppp1r15a Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2009AvailabilityAcademic Institutions and Nonprofits only