Addgene

We narrowed to 1,130 results for: SAM-2

Showing: 181 - 200 of 1130 results

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Mode #2 Library (NEDD4 WW2 domain)

Pooled Library

#111707

Purpose

Encodes NEDD4 WW2 domain and human phosphopeptides fused to split mCherry for Hi-P

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Expression

Bacterial

Species

Homo sapiens

Available since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only
SARS-CoV-2 spike G1 mutant

Plasmid

#231913

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G1 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G12345 mutant

Plasmid

#231920

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134, N1158, N1173, N1194 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G12345 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1134Q, N1158Q, N1173Q, N1194Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G13 mutant

Plasmid

#231915

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G13 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1158Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 spike G14 mutant

Plasmid

#231916

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G14 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1173Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SARS-CoV-2 spike G15 mutant

Plasmid

#231917

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1194 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G15 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1194Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SARS-CoV-2 spike G145 mutant

Plasmid

#231918

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173, N1194 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G145 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1173Q, N1194Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SARS-CoV-2 spike G1345 mutant

Plasmid

#231919

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158, N1173, N1194 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G1345 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1158Q, N1173Q, N1194Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
SARS-CoV-2 spike G12 mutant

Plasmid

#231914

Purpose

For expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134 by implementing Asn-to-Gln mutation

Depositor

Sriram Neelamegham

Article

Yang et al Npj Viruses. 2025 Jan 25;3(1):4. doi: 10

Insert

spike G12 (S SARS-CoV-2)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N1098Q, N1134Q

Promoter

CMV

Available since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry NEDD4-2 WW2

Plasmid

#111883

Purpose

Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Use

Tags

Split mCherry system: NEDD4-2 WW2 fused to C-term…

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 2 hU6 ACTB sgRNA

Plasmid

#206270

Purpose

ENTR Vector 2 for MultiSite Gateway assembly. Encodes hU6 hACTB sgRNA

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

ACTB sgRNA

Use

CRISPR; Multimate/gateway entr 2

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMMK ENTR 2 hU6 HEK3 PegRNA

Plasmid

#206277

Purpose

ENTR Vector 2 for MultiSite Gateway assembly. Encodes HEK3 PegRNA

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

HEK3 PegRNA

Use

CRISPR; Multimate/gateway entr 2

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 2 CMV mTFP1 Actin

Plasmid

#206255

Purpose

ENTR Vector 2 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes mTFP1 Actin under the control of CMV promoter.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mTFP1 Actin

Use

Multimate/gateway entr 2

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 2 CMV mKO2-hCdt1

Plasmid

#206282

Purpose

ENTR Vector 2 for MultiSite Gateway assembly. Encodes mKO2-hCdt1 under the control of CMV

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mKO2-hCdt1 (CDT1 Human)

Use

Multimate/gateway entr 2

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR207 SARS-CoV-2 NSP3 Cys857Ala_nostop

Plasmid

#154029

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP3

Use

Gateway-compatible entry vector, with mutagenized…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR207 SARS-CoV-2 NSP3 Cys857Ala

Plasmid

#154027

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP3

Use

Gateway-compatible entry vector, with mutagenized…

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

June 25, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221 SARS-CoV-2 S-frag5_nostop

Plasmid

#153177

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag5

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221 SARS-CoV-2 S-frag3_nostop

Plasmid

#153175

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag3

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221 SARS-CoV-2 S-frag4_nostop

Plasmid

#153176

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag4

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pDONR221 SARS-CoV-2 S-frag1_nostop

Plasmid

#153173

Purpose

Gateway-compatible Entry vector, with a fragmented insert of S gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth, Mikko Taipale

Article

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

S-frag1

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Many synonymous changes due to codon optimization

Promoter

Available since

May 27, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge