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pCMV-EGFP-T2A-ACREB-WPRE

Plasmid

#40867

Depositor

Harold Gainer

Publication

Lubelski et al Brain Res. 2012 Jan 6;1429:18-28. Epub 2

Insert

ACREB

Use

AAV

Promoter

CMV

Available since

Nov. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pSF3-microID

Plasmid

#172880

Purpose

expresses microID in mammalian cells with the tet-on or tet-off system

Depositor

Julien Béthune

Publication

Kubitz et al Commun Biol. 2022 Jul 4;5(1):657. doi: 1

Insert

microID

Use

Luciferase and Retroviral; To be used in a tet-on…

Tags

myc

Expression

Mammalian

Mutation

fragment ([aa2-171]) of aquifex aeolicus BirA wit…

Promoter

TRE-cmv

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast POLA1

Plasmid

#160807

Purpose

Express POLA1

Depositor

Richard Possemato

Publication

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

POLA1 (POLA1 Human)

Use

Retroviral

Mutation

Codon Optimized

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMT-ADARcd-E488Q-V5

Plasmid

#139686

Purpose

Expresses only the hyperactive form of the ADAR catalytic domain - no RNA binding protein. This is a control plasmid to be used with pMT-Hrp48-ADARcd-E488Q-V5

Depositor

Michael Rosbash

Publication

Xu et al RNA. 2018 Feb;24(2):173-182. doi: 10.126

Insert

Adenosine deaminase acting on RNA (Adar Fly)

Tags

V5

Expression

Bacterial and Insect

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pD21-AI-hLDLR

Plasmid

#124964

Purpose

Express human LDLR under human APOAI promoter

Depositor

Kazuhiro Oka

Publication

Li et al J Genet Syndr Gene Ther. 2011 Sep 28;2:1

Insert

human LDLR (LDLR Human)

Use

Adenoviral

Promoter

human APOAI

Available since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEF1a-Ronin-FLAG-IRES-Neo

Plasmid

#28020

Depositor

Thomas Zwaka

Publication

Dejosez et al Genes Dev. 2010 Jul 15. 24(14):1479-84.

Insert

Ronin (THAP11 Human)

Tags

FLAG

Expression

Mammalian

Available since

May 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MSI2-ADA

Plasmid

#166967

Purpose

Express the fusion protein of human MSI2 and the hyperactive catalytic domain of Drosophila ADAR

Depositor

Michael Kharas

Publication

Nguyen et al Nat Commun. 2020 Apr 24;11(1):2026. doi:

Insert

MSI2-ADA

Use

Retroviral

Tags

MSI2-ADA

Expression

Mammalian

Promoter

MSCV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NES

Plasmid

#124703

Purpose

Expresses MS2_adRNA targeting the RAB7A locus along with MCP_ADAR1 DD (E1008Q)_NES

Depositor

Prashant Mali

Publication

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E1008Q)_NES

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

E1008Q hyperactive mutant

Promoter

CMV

Available since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag OSM

Plasmid

#12191

Depositor

Gary Johnson

Publication

Uhlik et al Nat Cell Biol. 2003 Dec . 5(12):1104-10.

Insert

OSM (Ccm2 Mouse)

Tags

Flag

Expression

Mammalian

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_ADAR2DD_E488Q_Ago2

Plasmid

#205599

Purpose

expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) fused with human Ago2 in mammalian cells

Depositor

Marc Friedlander

Publication

Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

Insert

hAgo2 (AGO2 Human)

Tags

ADAR2DD_E488Q (hyperactive mutant)

Expression

Mammalian

Available since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-hyPE2

Plasmid

#177173

Purpose

Expresses hyPE2 in mammalian cells

Depositor

Hyongbum Kim

Publication

Song et al Nat Commun. 2021 Sep 23;12(1):5617. doi:

Insert

hyperPE2

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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MYH11-NanoLuc-2A-tdTomato

Plasmid

#132569

Purpose

Donor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele

Depositor

James Thomson

Publication

Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

Insert

Human MYH11 knockin homology arms (MYH11 Human)

Expression

Mammalian

Available since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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LifeAct-mhYFP

Plasmid

#186524

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to actin

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

LifeAct

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pSG5 EDD C2768A

Plasmid

#37192

Depositor

Darren Saunders, Charles Watts

Publication

Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

Insert

EDD C2768A (UBR5 Human)

Tags

6x His Tag and FLAG

Expression

Mammalian

Mutation

Changed Cysteine 2768 to Alanine; K503R (please s…

Promoter

SV40

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pPP076

Plasmid

#158794

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged CasΦ-1 in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Publication

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-1

Expression

Bacterial

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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Pinducer20-dCas9-SunTag-2A-BFP

Plasmid

#115779

Purpose

targeted DNA methylation tool

Depositor

Margaret Goodell

Publication

Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

Insert

dCas9-SunTag-2A-BFP

Expression

Mammalian

Promoter

TRE

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSPL3-ABCC8-2

Plasmid

#135916

Purpose

Encodes ABCC8 exon 2 wild type for the analysis of splicing variants

Depositor

Diana Valverde Pérez

Publication

Lago-Docampo et al Sci Rep. 2020 Sep 15;10(1):15135. doi: 1

Insert

ATP-binding Cassette subfamily C member 8 (ABCC8 Human)

Use

Exon trapping

Expression

Mammalian

Promoter

SV40

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

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lenti-HA-mBec1 F121A

Plasmid

#99506

Purpose

Lentiviral expression of mutant Becn1 leading to hyperactive autophagy

Depositor

Congcong He

Publication

Rocchi et al PLoS Genet. 2017 Aug 14;13(8):e1006962.

Insert

Beclin 1 (Becn1 Mouse)

Use

Lentiviral

Tags

HA

Mutation

F121A

Promoter

CMV

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_CaMKII-TRPV1-flag

Plasmid

#238924

Purpose

Expression of the human TRPV1 channel with a Flag-tag in excitatory neurons

Depositor

Vsevolod Belousov

Publication

Maltsev et al Cell Mol Life Sci. 2024 Oct 25;81(1):437

Insert

CaMKII-TRPV1-flag

Use

AAV

Tags

flag

Expression

Mammalian

Promoter

CaMKIIalfa

Available since

Aug. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Mito-mhYFP

Plasmid

#186526

Purpose

Localization of monomeric hyperfolder YFP fluorescent protein to mitochondria

Depositor

Gregory Petsko

Publication

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Monomeric hyperfolder YFP

Tags

Human COX8a first 29 amino acids

Expression

Mammalian

Mutation

hfYFP-S147P/L195M/V206K

Promoter

CMV

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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