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Plasmid#160579PurposeStreptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)DepositorInsertRDF
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK0_030
Plasmid#123969PurposeEncodes the phiC31-hygromycin-mRuby2PEST landing pad as a type 0 part to be used in the MTK systemDepositorInsertphiC31-hygro-mRuby2PEST landing
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
MTK0_009
Plasmid#123964PurposeEncodes PhiC31 landing pad as a type 0 part to be used in the MTK systemDepositorInsertphiC31 landing
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_033
Plasmid#123971PurposeEncodes the cROSA26 PhiC31 landing pad as a type 0 part to be used in the MTK systemDepositorInsertcROSA26 PhiC31 LP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_034
Plasmid#123972PurposeEncodes the hROSA26 PhiC31 landing pad as a type 0 part to be used in the MTK systemDepositorInserthROSA26 PhiC31 LP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK6_011
Plasmid#123865PurposeEncodes the inverted attB phiC31 transposon recognition site cassette as a type 6 part to be used in the MTK systemDepositorInsertattB (phiC31) inverted
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK6_007
Plasmid#123861PurposeEncodes the attB phiC31 transposon recognition site cassette as a type 6 part to be used in the MTK systemDepositorInsertattB (phiC31)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pB PUb-integrase
Plasmid#183966PurposepiggyBac plasmid for insect transformation, expressing PhiC31 integrase under control of the Aedes aegypti PUb promoter, GFP fluorescent markerDepositorInsertPhiC31 integrase
UsePiggybac insect transgenesis destination vectorTagsExpressionMutationPromoterAedes aegypti PolyubiquitinAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRDB_051
Plasmid#216081Purposefor stable fly cell lines; attL and attR sequences for genome integration by phiC31, destination vectorDepositorHas ServiceCloning Grade DNAInsertattL and attR sequences for genome integration by phiC31
UseDestinationTagsExpressionInsectMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha2 P35S:RDF:T35S (GB1508)
Plasmid#160613PurposeTU for the constitutive expression of Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)DepositorInsertRDF
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
MTK0_011
Plasmid#123966PurposeEncodes the hROSA26 PhiC31 recognition site with Hygromycin resistance and mRuby2 landing pad with GFP dropout destination vector as a type 0 part to be used in the MTK systemDepositorInserthRosa26-PhiC31 landing pad
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_052
Plasmid#123978PurposeEncodes the PhiC31 landing pad on hThal5.10-2-hg18 with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK systemDepositorInsertPhiC31 LP on hThal5.10-2-hg18 (Hygro::mRuby)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_054
Plasmid#123980PurposeEncodes the PhiC31 landing pad on hCLYBL with hygromycin resistance cassette coexpressed with mRuby2 as a type 0 part to be used in the MTK systemDepositorInsertPhiC31 LP on hCLYBL (Hygro::mRuby)
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRDB_053
Plasmid#216083Purposefor transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vectorDepositorHas ServiceCloning Grade DNAInsertgypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
UseDestinationTagsExpressionInsectMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T10 - T2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
Plasmid#219687PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, pUBQ10 promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/pUBQ10 (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
attB Neo* attP
Plasmid#138412PurposeattB and attP-flanked pPgk-pEM7-Neo*-bpA positive selection cassetteDepositorInsertNeo*
UseMouse Targeting; Att/phic31TagsExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)
Plasmid#219688PurposeLevel 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2BP/p35S (BFP, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
Plasmid#219686PurposeLevel 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).DepositorInsertT2PB/p35S (mTagBFP2, mCherry, NeonGreen)
UseTagsExpressionPlantMutationPromoterAvailable sinceJuly 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1_CBS-att-OCS-BeYDVRep-RepA-tNos (GB4643)
Plasmid#225437PurposeTranscriptional unit for the copper and PhiC31 regulated expression of BeYDV Rep/RepA.DepositorInsertCBS-att-OCS-BeYDVRep/RepA-tNos
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRAV-2
Plasmid#26747DepositorTypeEmpty backboneUseCre/Lox; Flp/frt; phic31/attb-attpTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2011AvailabilityAcademic Institutions and Nonprofits only